Viability of Oryza sativa L. seeds stored under genebank conditions for up to 30 years

Hay, Fiona R.; Guzman, F. de; Ellis, David; Makahiya, H.A. F.; Borromeo, Teresita H.; Hamilton, N. Ruaraidh Sackville

doi:10.1007/s10722-012-9833-7

Cited by 30 publications

(25 citation statements)

References 43 publications

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“…Research has shown that seed quality is influenced by genotype, environment during seed development, and seed storage conditions (Clerkx et al, 2004;Fessel et al, 2006;Açıkgöz et al, 2013;Hampton et al, 2013;Hay et al, 2013;Uddin et al, 2013). Our results suggest significant differences in longevity could not be found between either different species of the same genus (Diplotaxis tenuifolia and D. erucoides), or different seed lots of the same species (D. tenuifolia).…”

Section: Discussionmentioning

confidence: 99%

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Lazar¹,

Mira

Pamfil³

et al. 2014

Turk J Agric For

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The aim of this study was to determine whether the loss of germinability and vigour in wall-rocket seed lots was related to electrolyte leakage. Ten seed lots of 2 wall-rocket species (9 of Diplotaxis tenuifolia and one of D. erucoides (Brassicaceae)) were stored at 45 °C and 70% relative humidity and electrolyte leakage was measured using the conductivity test. Small differences in longevity were found among species or seed lots, suggesting that a similar relative longevity might be expected within the genus Diplotaxis after artificial ageing, and that it will be above the average of that found in other species of the same family at similar storage conditions. Electrolyte loss was strongly related to loss of seed viability. There was a negative linear relationship between conductivity and seed germination (R 2 = 0.85), and a positive relationship with T 50 (R 2 = 0.77). Small differences were found among species or seed lots regarding the correlation between electrolyte leakage and seed germination and vigour. Our results indicate that the conductivity method is able to predict the viability of wall-rocket seeds stored at 45 °C and 70% RH, with the same regression equation of conductivity and germination (-33.97x + 135.59) being able to predict the viability of different seed lots and species. A conductivity level ranging from 1.4 to 2.2 mS g -1 DW would indicate that the potential of a Diplotaxis seed lot to germinate has decreased to 80%. The correlation between electrolyte leakage and seed germination provides a valuable means for early detection of seed viability in wall-rocket seeds.

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Section: Discussionmentioning

confidence: 99%

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Lazar¹,

Mira

Pamfil³

et al. 2014

Turk J Agric For

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“…duration and environment of drying and prestorage environment; Hay & Probert 2011). Several studies describe the relative longevity of seeds in medium-and long-term gene bank storage (Walters et al 2005;Hay et al 2013;Ellis et al 2018). However, these studies relate to crop species with little information available for how long the seeds of native species can be stored without declines in viability and seedling vigor.…”

Section: Seed Longevitymentioning

confidence: 99%

Seed storage: maintaining seed viability and vigor for restoration use

Vitis

Hay

Dickie

et al. 2020

Restoration Ecology

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115

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Effective seed storage after sourcing (harvesting or purchasing) is critical to restoration practitioners and native seed producers, as it is key to maintaining seed viability. Inadequate seed storage can lead to a waste of both natural and economic resources when seeds of poor quality are sown. When working with native species with unknown storage behavior, general assumptions can be made based on studies on related species, and standard practices may be applied with caution; however, an investigation should be conducted to understand if specific storage requirements are needed and for how long seeds can be stored before they lose significant viability. In this paper of the Special Issue Standards for Native Seeds in Ecological Restoration, we provide an overview of the key concepts in seed storage and the steps to take for effective storage of native seeds for restoration use.

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“…At the T.T. Chang Genetic Resources Center in International Rice Research Institute, 183 rice accessions stored up to 30 years showed more than 70% germination5, and more than 93% of seed lots produced in 1980 still maintained 85% germination after 33 years in storage6. Owing to the reduction in seed viability, the regeneration of genetic resources is considered crucial for maintaining genetic integrity.…”

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confidence: 99%

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa

Yin

Xia

et al. 2017

Sci Rep

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The critical node (CN), which is the transition from the plateau phase to the rapid decreasing phase of seed ageing, is extremely important for seed conservation. Although numerous studies have investigated the oxidative stress during seed ageing, information on the changes in protein abundance at the CN is limited. In this study, we aimed to investigate the abundance and carbonylation patterns of proteins at the CN of seed ageing in rice. The results showed that the germination rate of seeds decreased by less than 20% at the CN; however, the abundance of 112 proteins and the carbonylation levels of 68 proteins markedly changed, indicating oxidative damage. The abundance and activity of mitochondrial, glycolytic, and pentose phosphate pathway proteins were reduced; consequently, this negatively affected energy production and germination. Proteins related to defense, including antioxidant system and heat shock proteins, also reduced in abundance. Overall, energy metabolism was reduced at the CN, leading to a decrease in the antioxidant capacity, whereas seed storage proteins were up-regulated and carbonylated, indicating that the seed had a lower ability to utilize seed storage proteins for germination. Thus, the significant decrease in metabolic activities at the CN might accelerate the loss of seed viability.A notable characteristic of seed viability is the reverse S-shaped survival curve during ageing, which includes a plateau phase (Phase I; P-I), followed by a rapid decreasing phase (Phase II; P-II) and a slow decreasing phase (Phase III; P-III). The transformation from P-I to P-II is defined as the critical node (CN), which is highly important for seed conservation 1 . The average germination of approximately 42,000 diverse accessions stored for 16 to 81 years at the National Center for Genetic Resources Preservation, USA has been decreased by 42% 2 . The average germination rate of peanut (stored for 34 years), soybean (stored for 36 years), wheat (stored for 43.6 years), and barley (stored for 44.4 years) is 6%, 21%, 73%, and 86%, respectively 3 . Similar results have been also reported by the Genebank of the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany 4 . Rice is extremely important food crop. One of main aims in genebanks is maintaining the rice seed safe conservation. At the T.T. Chang Genetic Resources Center in International Rice Research Institute, 183 rice accessions stored up to 30 years showed more than 70% germination 5 , and more than 93% of seed lots produced in 1980 still maintained 85% germination after 33 years in storage 6 . Owing to the reduction in seed viability, the regeneration of genetic resources is considered crucial for maintaining genetic integrity. Previous studies have shown that seed regeneration needs to be carried out prior to the CN in order to prevent a large decrease in viability, which can lead to changes in genetic composition 7,8 . Previously, we showed that the mitochondrial ultrastructure of seed at the CN is abnormal owing to the decre...

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Viability of Oryza sativa L. seeds stored under genebank conditions for up to 30 years

Cited by 30 publications

References 43 publications

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species

Seed storage: maintaining seed viability and vigor for restoration use

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa

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