Viability of Metarhizium anisopliae conidia (metsch.) Sorok preserved in packages containing silica gel

Freitas, Aline de Araújo; Araújo, Maria Gabriela Bispo Almeida; Hendges, Eleci Adriano; Azevedo, A. G. C. de; Lima, Danielle Marques de Oliveira; Cruz, Ivan; Mendonça, Marcelo da Costa; Diniz, Leandro Eugênio Cardamone

doi:10.1186/1753-6561-8-s4-p128

Cited by 4 publications

(1 citation statement)

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“…Formulation and application methodology may also be used to increase larval mortality following field application with the fungus. Dry spore applications would be more susceptible to external environmental factors including heat and UV which are known to negatively influence fungal stability ( Braga et al 2007 , de Freitas et al 2014 ). In these instances, oil formulations may offer some protection from these abiotic factors; neem-oil formulation, for instance, has been shown to protect the spores from UV exposure for up to two hours ( Paula et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Optimizing the Application Timing and Dosage ofMetarhizium brunneum(Hypocreales: Clavicipitaceae) as a Biological Control Agent ofAedes aegypti(Diptera: Culicidae) Larvae

Alkhaibari

Wood

Yavaşoğlu

et al. 2022

Journal of Medical Entomology

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Aedes aegypti (Diptera: Culicidae) is the principal vector of dengue and other viruses that cause disease among 100 to 400 million people each year. The recent development of widespread insecticidal resistance has led to the rapid development of biological control solutions aimed at larval control. While the efficacy of Metarhizium brunneum has been shown against Aedes larvae, the impact of larval population dynamics will need to be determined to formulate effective control strategies. In this study, larvae were subjected to four concentrations of M. brunneum (105, 106, 107, 108 conidia ml−1). Larvae were found to be susceptible to M. brunneum with dose-dependent efficacy. When constant larval immigration was added as a parameter, peak mortality was consistently found to occur on the fourth day, before a significant reduction in control efficacy linked to a decline in conidial availability within the water column. This suggests that M. brunneum treatments should be applied at a concentration 1 × 107 conidia ml−1 every four days to effectively control mosquito larvae in the field, regardless of the fungal formulation, water volume, or larval density. Understanding fungal-mosquito dynamics is critical in developing appropriate control programs as it helps optimize the fungal control agent’s dose and frequency of application.

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Section: Discussionmentioning

confidence: 99%