Viability of metacercariae of Clonorchis sinensis in frozen or salted freshwater fish

Fan, P. C.

doi:10.1016/s0020-7519(97)00215-4

Cited by 45 publications

(31 citation statements)

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“…This infection is also becoming increasingly common in non-endemic regions and in developed countries due to growing international markets, improved transportation systems, and demographic changes such as population movements [2,3]. Epidemiological data suggested that clonorchiasis has an increasing human-health impact resulted from the greater consumption of raw, frozen, dried, or pickled freshwater fish imported from endemic areas [1,4]. …”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of microRNAs in Clonorchis sinensis of human health significance

Liu²,

Nisbet

et al. 2010

BMC Genomics

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BackgroundClonorchis sinensis is a zoonotic parasite causing clonorchiasis-associated human disease such as biliary calculi, cholecystitis, liver cirrhosis, and it is currently classified as carcinogenic to humans for cholangiocarcinoma. MicroRNAs (miRNAs) are non-coding, regulating small RNA molecules which are essential for the complex life cycles of parasites and are involved in parasitic infections. To identify and characterize miRNAs expressed in adult C. sinensis residing chronically in the biliary tract, we developed an integrative approach combining deep sequencing and bioinformatic predictions with stem-loop real-time PCR analysis.ResultsHere we report the use of this approach to identify and clone 6 new and 62,512 conserved C. sinensis miRNAs which belonged to 284 families. There was strong bias on families, family members and sequence nucleotides in C. sinensis. Uracil was the dominant nucleotide, particularly at positions 1, 14 and 22, which were located approximately at the beginning, middle and end of conserved miRNAs. There was no significant "seed region" at the first and ninth positions which were commonly found in human, animals and plants. Categorization of conserved miRNAs indicated that miRNAs of C. sinensis were still innovated and concentrated along three branches of the phylogenetic tree leading to bilaterians, insects and coelomates. There were two miRNA strategies in C. sinensis for its parasitic life: keeping a large category of miRNA families of different animals and keeping stringent conserved seed regions with high active innovation in other places of miRNAs mainly in the middle and the end, which were perfect for the parasite to perform its complex life style and for host changes.ConclusionsThe present study represented the first large scale characterization of C. sinensis miRNAs, which have implications for understanding the complex biology of this zoonotic parasite, as well as miRNA studies of other related species such as Opisthorchis viverrini and Opisthorchis felineus of human and animal health significance.

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Section: Introductionmentioning

confidence: 99%

Identification and characterization of microRNAs in Clonorchis sinensis of human health significance

Liu²,

Nisbet

et al. 2010

BMC Genomics

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“…Fish muscle was completely removed and digested in pepsin solution for an hour at 37 • C as by Fan [22]. The digested materials were filtered and precipitated with saline.…”

Section: Challenge Infections and Parasite Burdensmentioning

confidence: 99%

Oral administration of a Bacillus subtilis spore-based vaccine expressing Clonorchis sinensis tegumental protein 22.3kDa confers protection against Clonorchis sinensis

Zhou

Xia

et al. 2008

Vaccine

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“…It is currently estimated that more than 200 million people are at risk of infection and ~20 million are infected globally 3, 4 . Humans are mainly infected via consumption of undercooked (including dried, salted, smoked, or pickled) or raw infected fish 2, 5–8 . The symptoms of human clonorchiasis include indigestion, fullness of the abdomen, loss of appetite, epigastric distress unrelated to meals, diarrhea, edema, hepatomegaly, and toxemia from liver impairment.…”

Section: Introductionmentioning

confidence: 99%

Structural insights into a 20.8-kDa tegumental-allergen-like (TAL) protein from Clonorchis sinensis

Son

Kim

et al. 2017

Sci Rep

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Survival of Clonorchis sinensis, a cause of human clonorchiasis, requires tegument proteins, which are localized to the tegumental outer surface membrane. These proteins play an important role in a host response and parasite survival. Thus, these proteins are interesting molecular targets for vaccine and drug development. Here, we have determined two crystal structures of the calmodulin like domain (amino acid [aa] positions 1–81) and dynein light chain (DLC)-like domain (aa 83–177) of a 20.8-kDa tegumental-allergen-like protein from Clonorchis sinensis (CsTAL3). The calmodulin like domain has two Ca2+-binding sites (named CB1 and CB2), but Ca2+ binds to only one site, CB1. The DLC-like domain has a dimeric conformation; the interface is formed mainly by hydrogen bonds between the main chain atoms. In addition, we have determined full-length structure of CsTAL3 in solution and showed the conformational change of CsTAL3 induced by Ca2+ ion binding using small-angle X-ray scattering analysis and molecular dynamics simulations. The Ca2+-bound form has a more extended conformation than the Ca2+-free from does. These structural and biochemical analyses will advance the understanding of the biology of this liver fluke and may contribute to our understanding of the molecular mechanism of calcium-responsive and tegumental-allergen-like proteins.

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Viability of metacercariae of Clonorchis sinensis in frozen or salted freshwater fish

Cited by 45 publications

References 1 publication

Identification and characterization of microRNAs in Clonorchis sinensis of human health significance

Identification and characterization of microRNAs in Clonorchis sinensis of human health significance

Oral administration of a Bacillus subtilis spore-based vaccine expressing Clonorchis sinensis tegumental protein 22.3kDa confers protection against Clonorchis sinensis

Structural insights into a 20.8-kDa tegumental-allergen-like (TAL) protein from Clonorchis sinensis

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