Viability of lyophilized microorganisms after storage

Antheunisse, J.

doi:10.1007/bf02578856

Cited by 38 publications

(12 citation statements)

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“…Bacterial recombinants are currently preserved by freezing [27][28][29] and techniques for the long-term preservation of bacterial cultures have been devised, 27,30-33 including stab culture 34 and lyophilization. 35,36 However, viability may decrease with prolonged storage. 37,38 It was found that cryopreservation with glycerol even appeared to diminish viability, 29 although this method is widely used.…”

Section: Discussionmentioning

confidence: 98%

DNA Shuttling Between Plasmid Vectors and a Genome Vector: Systematic Conversion and Preservation of DNA Libraries Using the Bacillus subtilis Genome (BGM) Vector

Kaneko

Akioka

Tsuge

et al. 2005

Journal of Molecular Biology

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Section: Discussionmentioning

confidence: 98%

DNA Shuttling Between Plasmid Vectors and a Genome Vector: Systematic Conversion and Preservation of DNA Libraries Using the Bacillus subtilis Genome (BGM) Vector

Kaneko

Akioka

Tsuge

et al. 2005

Journal of Molecular Biology

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“…The ampoules in these previous studies were sealed under a high vacuum. We described how our freeze-dried bacteria sealed in ampoules at 1 Pa lived longer than those sealed at approximately 7 Pa (Antheunisse, 1973); survival duration was positively correlated with vacuum pressure (Antheunisse, 1973;Miyamoto-Shinohara et al, 2006). In this study, we investigated the effect of vacuum pressure and storage temperature on the survival rates of various yeast strains.…”

Section: Introductionmentioning

confidence: 99%

Survival of yeasts stored after freeze-drying or liquid-drying

Miyamoto‐Shinohara¹,

Nozawa²,

Sukenobe³

et al. 2010

J. Gen. Appl. Microbiol.

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IntroductionFreeze-drying and L-drying are popular methods of preserving microorganisms, because long-term viability is excellent in most cases and the storage and distribution requirements are simple. By plotting the survival curves of freeze-dried species sealed under high vacuum ( 1 Pa) and stored at 5 C in the dark, we previously found that the survival rates of Gram-positive bacteria immediately after freeze-drying and during storage were higher than those of Gram-negative bacteria (Miyamoto-Shinohara et al., 2008). In addition, the survival rate of the yeast Saccharomyces cerevisiae immediately after freeze-drying was lower than those of bacterial species, although S. cerevisiae survival rates were stable during storage (Miyamoto-Shinohara et al., 2000.To increase the survival rates of S. cerevisiae strains, we tested the applicability of L-drying, in which cells are desiccated by vacuum evaporation at room temperature. In contrast, during freeze-drying, cells are desiccated by sublimation. L-drying is effective for the long-term preservation of cultures sensitive to freezedrying (Malik, 1990(Malik, , 1999. In this research, yeast strains were L-dried according to the method of the Institute for Fermentation, Osaka (Banno et al., 1979), in which the cell suspension was dispensed into a glass ampoule (in the same way as for freeze-drying) and a cotton wool plug was inserted to prevent the temperature in the ampoule from falling to the freezing point (Iijima and Sakane, 1973).To clarify the mechanisms underlying tolerance to L-drying and freeze-drying, we investigated the survival of type strains of various yeast species. The survival J. Gen. Appl. Microbiol., 56, 107 119 (2010) We investigated

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“…We have tried to establish the survival rates of different microbes in an effort to improve our methods of freeze-drying. At IPOD, freeze-dried microorganisms are sealed in ampoules under a vacuum (Ͻ1 Pa) and stored in the dark at 5°C; such conditions allow the microorganisms to survive for a longer time during storage than does sealing under approximately 7 Pa (Antheunisse, 1973;Miyamoto-Shinohara et al, 2006). Because the deposited strains represent a broad range of taxa, we have been evaluating the survival rates after freezedrying and the survival rates during storage for each species in the collection (Miyamoto-Shinohara et al, 2000, 2006.…”

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confidence: 99%