Viability of cattle sperm under different storage conditions in Cameroon

Abstract

A study was carried out to evaluate the viability of extended cattle semen, without freezing, under different storage conditions. The semen was collected from Holstein Friesian bulls using artificial vaginas. The semen was extended and stored in a 3-by-4 factorial design (storage system × ice change). The storage media were ice boxes, buckets, and refrigerator. The ice in these media was either replaced daily, on the first and third day, first day only, or no ice at all after the semen collection. Results showed an overwhelming evidence of the effect of storage medium and ice change on sperm viability (P<0.0001). Individual motility before processing was highest in the refrigerator with averages of 44.5%, 39.5% in ice boxes, and 10% in buckets during the 8-day experiment. There was no significant difference (P>0.05) in progressive motility after processing in the refrigerator (34%) and in ice boxes (33%) but significantly higher (P<0.01) to the 10% obtained in buckets. It was shown that spermatozoa in the ice box retained 45% individual motility up to the sixth day after semen was collected on the condition that the ice was changed on the third day. Progressive motility after processing in the ice box was 40% up to the sixth day with the ice changed on the third day while the spermatozoa were well preserved up to the fourth day in the same medium if the ice put on the first day was not changed. This study shows that, if farmers plan to inseminate cows within the first day after semen collection, they can use buckets with ice for the transportation of the extended semen ampoules from the artificial insemination center. Otherwise, the semen needs to be kept in ice boxes and the ice changed on the third day after collection and this semen could be used within a week.

Kinetic Motilities of Cryopreserved Bull Spermatozoa: Owing to the Effect of <i>Eurycoma longifolia</i> Jack Aqueous Extract

“…On the other hand, we evaluated the kinetic motilities of frozenthawed semen into three different times: results showed that time of evaluation did not differ between periods of evaluation i.e., the storage semen in frozen form at (-196°C) using liquid nitrogen will not change the kinetic values of sperm even stored for long period. This is in contrast with semen stored in chilled form even in different storage conditions (Bayemi et al, 2010).…”

Pengaruh Albumin Telur dari Berbagai Jenis Unggas Sebagai Pengganti BSA (Bovine Serum Albumin) dalam Pengencer CEP Terhadap Kualitas Spermatozoa Kambing Peranakan Etawa (PE) Pada Suhu Penyimpanan 4-5°C

“…Pengamatan motilitas mengikuti metode Garner dan Hafez (2008) dengan mengestimasi persentase pergerakan spermatozoa maju ke depan (progresif) yang dinilai oleh 2 orang lalu diambil rataratanya. Menurut Bayemi et al (2010) pengujian motilitas dilakukan dengan cara semen diteteskan pada gelas objek lalu ditutup menggunakan cover penutup selanjutnya diamati dengan mikroskop perbesaran 400x.…”

Effect of Weaning Age on the Survival Rate and Growth Performance of Rabbits Kept in the Western Highlands of Cameroon

“…The average maximum and minimum temperatures are respectively 25°C and 14°C. The average relative humidity is 52% in the dry seasons and 70% in the wet seasons [8].…”