Viability of black currant pollen before and after cryopreservation in liquid nitrogen, and its morphological features

Tikhonova, O. A.; Гаврилова, О. А.; Радченко, Е.; Вержук, В. Г.; Pavlov, A. V.

doi:10.30901/2227-8834-2020-3-110-119

Cited by 6 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most effective method of ex situ storage of fruit and berry crops with vegetative propagation is cryopreservation in liquid nitrogen (LN, −196 °C) or its vapor phase (−183–−185 °C) [ 8 , 9 , 10 , 11 ]. This method was successfully applied for the long-term storage of meristem and pollen of the blackcurrant, apple, sweet cherry, and plum [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. Cryopreservation using winter-dormant vegetative buds was implemented to back-up field collections of Malus in the USA [ 21 , 22 ], Canada [ 23 ], and Germany [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Conservation of the Bird Cherry (Padus Mill.) Germplasm by Cold Storage and Cryopreservation of Winter Cuttings

Verzhuk,

Murashev,

Novikova

et al. 2023

Biology

Self Cite

View full text Add to dashboard Cite

Conservation at cryogenic temperatures, usually in liquid nitrogen (LN) or in its vapor, is the only reliable method for the long-term ex situ conservation of fruit and berry crops with vegetative reproduction. In this study, five bird cherry (Padus Mill.) varieties of different genetic origin from the bird cherry genebank at the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR, Russia) were tested for their response to cryopreservation in LN vapor (−183–−185 °C). The response included viability under laboratory and field conditions, morphological assessment of the developed plants and biochemical analysis of fruits produced during three consecutive years by plants developed from cryopreserved cuttings. All parameters were compared to those recorded after cold storage of cuttings (−5 °C), a routine mid-term conservation method currently used at the VIR genebank. The initial viability of winter cuttings varied from 86.7% to 93.3%. Six-month cold storage and cryopreservation reduced viability to 53.3–86.7% and 43.3–60.0%, respectively, which was above the 40% viability threshold in all varieties tested. Cuttings after cold storage showed better viability when recovered in the laboratory (80% mean viability) than in the field (58% mean viability); viability of cryopreserved cuttings was not affected by recovery conditions. The results of a two-way analysis of covariance suggested that storage and recovery conditions had the most significant effect on viability (p < 0.0001), while the effects of genotype (p = 0.062) and factor interactions (p = 0.921) were minor. Cryopreservation had little or no influence on morphological parameters of the plants recovered in the field, including plant height, number of shoots, internodes and roots, and root length. Similarly, no effect of cryopreservation was recorded on dry matter content, total sugar content and ascorbic acid concentration in fruits produced by plants developed from the cryopreserved cuttings. These results suggest that cryopreservation in LN vapor is a reliable method for conservation of the bird cherry genetic collection and is worth testing with a broader variety of genotypes.

show abstract

Section: Introductionmentioning

confidence: 99%

Conservation of the Bird Cherry (Padus Mill.) Germplasm by Cold Storage and Cryopreservation of Winter Cuttings

Verzhuk,

Murashev,

Novikova

et al. 2023

Biology

Self Cite

View full text Add to dashboard Cite

show abstract

Initial and post-cryogenic assessment of viability of russian plum cultivars pollen (Prunus rossica Erem.) in the conditions of the North-West of Russia

et al. 2021

View full text Add to dashboard Cite

The viability of pollen of 5 collectible varieties of Russian plum of the Pushkinskiye and Pavlovskiye VIR Laboratories SPB before and after cryopreservation was studied. It was found that a significant factor for the level of pollen viability is “variety” (p=0.07), the factors “cryopreservation” (p=0.457), “year” (p=0.221), as well as the interaction of the factors “variety× cryopreservation” (p=0.172), were not significant at the p<0.05 level of significance. For long-term storage of Russian plum pollen in liquid nitrogen, the year of pollen collection is not a significant factor. For most genotypes, no negative impact of cryoсonservation on pollen viability was revealed. The varieties ‘Aureus’, 'Podarok Sankt-Peterburgu’ and ‘Exotica’ after cryopreservation for 1 year showed a tendency to increase the pollen viability indicators.

show abstract

Reproductive ability of black currant cultivars after pollen cryopreservation in liquid nitrogen

Tikhonova

Радченко

Pavlov

2021

Trudy po prikladnoy botanike, genetike i selektsii

Self Cite

View full text Add to dashboard Cite

Background. Studying the reproductive ability of black currant cultivars after long-term cryopreservation of their pollen in liquid nitrogen is very important for plant diversity conservation and for the effectiveness of breeding efforts.Materials and methods. Five black currant cultivars served as the material for the research. The viability of black currant pollen after cryopreservation in liquid nitrogen was analyzed. Pollen viability was tested on an artificial medium containing 10% of sucrose and 0.8% of agar. The viability of pollen was tested on the black currant collection at Pushkin and Pavlovsk Laboratories of VIR. The cultivar ‘Andreevskaya’ (k-15630) was pollinated with pollen stored for one year in liquid nitrogen (–196°C) as well as with fresh pollen (control test).Results and conclusion. After 12 months of conservation under ultralow temperatures there was a change in the level of pollen viability depending on the genotype: it varied from 10.4% (cv. ‘Pozdnyaya poslevoennaya’, k-7654) to 50.4% (cv. ‘Kriviai’, k-42517), while its mean value was 0.9% higher than the mean initial viability. The fruit setting rate in the control option was 81.3–94.2%. When pollinated with pollen kept in liquid nitrogen for one year, the fruit setting rate varied from 69.2% (‘Kacha’, k-44185) to 93.3% (‘Belorusochka’, k-41978); the remaining cultivars (‘Pozdnyaya poslevoennaya’; ‘Chereshneva’, k-42481; and ‘Kriviai’) demonstrated intermediate values of this indicator, i.e., the reproductive ability of pollen after cryopreservation was high even despite its low viability. As for the fruit weight, no differences were shown by cvs. ‘Kacha’, ‘Chereshneva’ and ‘Kriviai’ in both pollination options, while this indicator in cvs. ‘Belorusochka’ and ‘Pozdnyaya poslevoennaya’ was lower than the control by 0.31 and 0.24 g, respectively.

show abstract

Viability of black currant pollen before and after cryopreservation in liquid nitrogen, and its morphological features

Cited by 6 publications

References 8 publications

Conservation of the Bird Cherry (Padus Mill.) Germplasm by Cold Storage and Cryopreservation of Winter Cuttings

Conservation of the Bird Cherry (Padus Mill.) Germplasm by Cold Storage and Cryopreservation of Winter Cuttings

Initial and post-cryogenic assessment of viability of russian plum cultivars pollen (Prunus rossica Erem.) in the conditions of the North-West of Russia

Reproductive ability of black currant cultivars after pollen cryopreservation in liquid nitrogen

Contact Info

Product

Resources

About