Viability of allergy (IgE) detection using an alternative aptamer receptor and electrochemical means

Papamichael, Katerina I.; Kreuzer, Mark P.; Guilbault, George G.

doi:10.1016/j.snb.2006.09.024

Cited by 58 publications

(37 citation statements)

References 35 publications

(38 reference statements)

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“…The sandwich assay for detection IgE using electrochemical aptasensor was proposed in detailed study of Papamichael et al [78]. In this work the IgE specific antibody was immobilized on a surface of screen-printed electrode.…”

Section: Electrochemical Aptasensors Based On Aptamers Conjugated Witmentioning

confidence: 99%

Electrochemical Aptasensors – Recent Achievements and Perspectives

2009

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This article reviews recent achievements in developing aptamer-based electrochemical biosensors (electrochemical aptasensors). Aptamers are single stranded DNA or RNA molecules with high specificity to various ligands. Their specificity is comparable and in certain cases even higher than those of antibodies. In contrast to antibodies, aptamers are prepared by an in vitro selection procedure developed simultaneously in the early 1990s by L. Gold and A. Ellington. Due to their stability and the possibility of chemical modification aptamers can be immobilized on various supports and serve as artificial receptors in biosensors. The first aptasensors developed in the second half of 1990s were based on optical detection. However, in early 2000 substantial interest arose to the development of electrochemical aptasensors. It has been shown that due to their simplicity and fast response they represent an excellent tool in practical applications. The main focus of this review is to discuss the configuration of aptamers and electrochemical methods for detecting aptamers -analyte interactions. We will also provide a brief history of aptamer development, along with molecular structure and methods of aptamer engineering. Methods for immobilizing aptamers onto a solid support are also discussed.

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Section: Electrochemical Aptasensors Based On Aptamers Conjugated Witmentioning

confidence: 99%

Electrochemical Aptasensors – Recent Achievements and Perspectives

2009

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“…A sandwich format has been designed. Thiolated aptamers A disposable electrochemical aptasensor using differential pulse voltammetry (DPV) as the transduction mode and based on a competitive instead of a sandwich format has also been reported for IgE detection [19]. This system used alkaline phosphatase as the enzyme label.…”

Section: Enzyme Labelsmentioning

confidence: 99%

Electrochemical Aptasensors

2009

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This review presents electrochemical aptasensors reported in the literature. Their development has been growing as evidenced by the increasing number of scientific publications on this subject since 2004. Aptasensors are presented according to their transduction mode, including the most described amperometric and impedimetric devices as well as FETs and the first very recently reported potentiometric aptasensor. Signal-on (positive readout signal) and less sensitive signal-off (negative readout signal) aptasensors are reviewed based on target binding-induced conformational change of aptamers or on target binding-induced strand displacement or even on both processes. Aptamerlabeled and aptamer label-free devices are presented separately.

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“…Aptamers bind proteins with high affinity representing thus interesting alternatives to antibodies [135]. Aptamers are small (usually 40 to 100 nucleotides) synthetic oligonucleotides that can specifically recognize and bind to virtually any kind of target, including whole cells, low-molecularweight ligands, peptides, and proteins [136 -143].…”

Section: Aptamers and Dna-binding Proteinsmentioning

confidence: 99%

Electroactivity of Nonconjugated Proteins and Peptides. Towards Electroanalysis of All Proteins

2007

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Present proteomics and biomedicine require sensitive analytical methods for all proteins. Recent progress in electrochemical analysis of peptides and proteins based on their intrinsic electroactivity is reviewed. Tyrosine and/or tryptophan-containing proteins are oxidizable at carbon electrodes. At mercury electrodes all peptides and proteins (about 13 peptides and > 25 proteins were tested) produce chronopotentiometric peak H at nanomolar concentrations. This peak is sensitive to changes in protein structure. Microliter sample volumes are sufficient for the analysis. Electrochemical methods can be used in studies of nucleic acid-protein interactions and can be applied in biomedicine. Examples of such applications in neurogenerative diseases and cancer are presented.

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Viability of allergy (IgE) detection using an alternative aptamer receptor and electrochemical means

Cited by 58 publications

References 35 publications

Electrochemical Aptasensors – Recent Achievements and Perspectives

Electrochemical Aptasensors – Recent Achievements and Perspectives

Electrochemical Aptasensors

Electroactivity of Nonconjugated Proteins and Peptides. Towards Electroanalysis of All Proteins

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