Viability monitoring intervals for genebank samples of Oryza sativa

Hay, Fiona R.; Guzman, F. de; Hamilton, N. Ruaraidh Sackville

doi:10.15258/sst.2015.43.2.17

Cited by 14 publications

(13 citation statements)

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“…At the T.T. Chang Genetic Resources Center in International Rice Research Institute, 183 rice accessions stored up to 30 years showed more than 70% germination5, and more than 93% of seed lots produced in 1980 still maintained 85% germination after 33 years in storage6. Owing to the reduction in seed viability, the regeneration of genetic resources is considered crucial for maintaining genetic integrity.…”

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confidence: 99%

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa

Yin

Xia

et al. 2017

Sci Rep

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The critical node (CN), which is the transition from the plateau phase to the rapid decreasing phase of seed ageing, is extremely important for seed conservation. Although numerous studies have investigated the oxidative stress during seed ageing, information on the changes in protein abundance at the CN is limited. In this study, we aimed to investigate the abundance and carbonylation patterns of proteins at the CN of seed ageing in rice. The results showed that the germination rate of seeds decreased by less than 20% at the CN; however, the abundance of 112 proteins and the carbonylation levels of 68 proteins markedly changed, indicating oxidative damage. The abundance and activity of mitochondrial, glycolytic, and pentose phosphate pathway proteins were reduced; consequently, this negatively affected energy production and germination. Proteins related to defense, including antioxidant system and heat shock proteins, also reduced in abundance. Overall, energy metabolism was reduced at the CN, leading to a decrease in the antioxidant capacity, whereas seed storage proteins were up-regulated and carbonylated, indicating that the seed had a lower ability to utilize seed storage proteins for germination. Thus, the significant decrease in metabolic activities at the CN might accelerate the loss of seed viability.A notable characteristic of seed viability is the reverse S-shaped survival curve during ageing, which includes a plateau phase (Phase I; P-I), followed by a rapid decreasing phase (Phase II; P-II) and a slow decreasing phase (Phase III; P-III). The transformation from P-I to P-II is defined as the critical node (CN), which is highly important for seed conservation 1 . The average germination of approximately 42,000 diverse accessions stored for 16 to 81 years at the National Center for Genetic Resources Preservation, USA has been decreased by 42% 2 . The average germination rate of peanut (stored for 34 years), soybean (stored for 36 years), wheat (stored for 43.6 years), and barley (stored for 44.4 years) is 6%, 21%, 73%, and 86%, respectively 3 . Similar results have been also reported by the Genebank of the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany 4 . Rice is extremely important food crop. One of main aims in genebanks is maintaining the rice seed safe conservation. At the T.T. Chang Genetic Resources Center in International Rice Research Institute, 183 rice accessions stored up to 30 years showed more than 70% germination 5 , and more than 93% of seed lots produced in 1980 still maintained 85% germination after 33 years in storage 6 . Owing to the reduction in seed viability, the regeneration of genetic resources is considered crucial for maintaining genetic integrity. Previous studies have shown that seed regeneration needs to be carried out prior to the CN in order to prevent a large decrease in viability, which can lead to changes in genetic composition 7,8 . Previously, we showed that the mitochondrial ultrastructure of seed at the CN is abnormal owing to the decre...

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confidence: 99%

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa

Yin

Xia

et al. 2017

Sci Rep

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“…For a given seed lot in hermetic storage at one moisture content, reducing storage temperature from 8 to -18°C would be estimated to increase longevity five-fold (precise value, 5.38). Note, however, that Hay et al (2015) provided examples in rice (Oryza sativa L.) where survival in the base was superior to that in the active collection, but in a few accessions, surprisingly, survival in the active was greater than in the base collection.…”

Section: Discussionmentioning

confidence: 99%

“…Frequent germination tests to monitor accessions are also costly in terms of labour and deplete valuable seed, and low seed numbers requires premature regeneration. Hence, monitoring tests should be as infrequent as prudent management allows, and so estimates of longevity in long-and medium-term stores are required for the effective management of a genebank (Hay et al 2015), particularly in wild species where the risk of losing the genotype may be high (Probert et al 2009). Seed dormancy may also be high in wild species and advice on breaking dormancy and promoting the germination of diverse species in genebank monitoring tests has been provided (Ellis et al 1985a, b).…”

Section: Introductionmentioning

confidence: 99%

“…Several have reported results from several decades monitoring accessions in medium-and/or long-term stores. Those reports have shown evidence of: considerable variation in seed longevity amongst contrasting species in medium-long-term stores (Walters et al 2005); variation amongst species with considerable loss in viability for some in medium-term stores (Lee et al 2013); benefit to seed survival in long-compared with medium-term stores (Agacka et al 2014), or shown this often but not consistently so (Hay et al 2013(Hay et al , 2015; considerable variation in seed survival amongst accessions within a species (Hay et al 2013(Hay et al , 2015; excellent seed survival after 40 years at the recommended long-term seed store conditions (Pérez-García et al 2007) and hence survival periods could not be quantified.…”

Section: Introductionmentioning

confidence: 99%

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Medium-term seed storage of 50 genera of forage legumes and evidence-based genebank monitoring intervals

Ellis

Nasehzadeh

Hanson³

et al. 2017

Genet Resour Crop Evol

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Genebanks maintaining seeds for longterm genetic resources conservation monitor seed lots to detect early loss in viability. Monitoring is costly and depletes valuable seed. Three decades of genebank seed germination test results of diverse forage species from 50 legume genera in the International Livestock Research Institute's medium-term store (circa 8°C with 5% moisture content) were analysed to determine whether advice on seed monitoring intervals could be derived. Cumulative normal distributions were fitted by probit analysis for each seed lot and compared within each genus. Six patterns of within-genus variation were identified: no detectable trend in germination test results during storage (4 genera); detectable trends, but variable (positive to negative) amongst lots (5); consistent slope of loss in viability amongst lots (17); consistent slope of increase in ability to germinate amongst lots (21); common loss in viability amongst lots (2); common increase in ability to germinate amongst lots (1). Seed lot monitoring intervals for the medium-term store were derived for each of 19 genera with consistent loss in viability across seed lots: three genera provided comparatively rapid deterioration, five met the general expectations for a medium-term store (2-10 years' maintenance of high viability), whilst 11 provided much better survival. Moreover, 26 further genera provided no evidence as yet of seed deterioration; of these, 22 improved in ability to germinate during storage indicating confounding of hardseededness with viability in germination tests.

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“…A number of genebanks have now published historical viability monitoring data for seeds in medium-and/or long-term storage, including the United States Department of Agriculture [36]; the National Agrobiodiversity Center of South Korea [37]; the Centre for Genetic Resources, the Netherlands (CGN [38,39]); the International Rice Research Institute (IRRI [40]); and the International Livestock Research Institute (ILRI [41,42]). Some of these publications highlight the problems of analysing such data, including changes in protocols and/or storage conditions, failure to overcome dormancy, and censoring.…”

Section: Historical Viability Monitoring Datamentioning

confidence: 99%

Why Seed Physiology Is Important for Genebanking

Whitehouse

Hay

Lusty³

2020

Plants

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Genebank management is a field in its own right; it is multifaceted, requiring a diverse set of skills and knowledge. Seed physiology is one area that is critical to the successful operation of seed genebanks, requiring understanding of seed quality during development and maturation, seed dormancy and germination, and seed longevity in storage of the target species. Careful management of the workflow between these activities, as seeds move from harvest to storage, and the recording and management of all relevant associated data, is key to ensuring the effective conservation of plant genetic resources. This review will discuss various aspects of seed physiology that genebank managers should be aware of, to ensure appropriate decisions are made about the handling and management of their seed collections.

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Viability monitoring intervals for genebank samples of Oryza sativa

Cited by 14 publications

References 0 publications

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa

Proteomic and Carbonylation Profile Analysis at the Critical Node of Seed Ageing in Oryza sativa

Medium-term seed storage of 50 genera of forage legumes and evidence-based genebank monitoring intervals

Why Seed Physiology Is Important for Genebanking

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