Viability markers for determination of desiccation tolerance and critical stages during dehydration in <i>Selaginella</i> species

Alejo-Jacuinde, Gerardo; Kean-Galeno, Tania; Martínez‐Gallardo, Norma; Tejero-Díez, J. Daniel; Mehltreter, Klaus; Délano‐Frier, John Paul; Oliver, Melvin J.; Simpson, June; Herrera-Estrella, Luis

doi:10.1093/jxb/erac121

Cited by 3 publications

(1 citation statement)

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“…Controlled conditions, here used as a collective name for greenhouse and growth chamber experiments, allow higher throughput, increased speed of innovation and reduced cost compared with field trials ( Simmons et al, 2021 ). In addition, more detailed mechanistic insights and mode of action studies often require controlled conditions, where responses to changes in the environment can be monitored with respect to time, space, amplitude, and other factors related to both the stress applied and the response output ( Verslues et al, 2006 ; Bao et al, 2014 ; Alejo-Jacuinde et al, 2022 ; Hu et al, 2022 ). On the other hand, field trials provide valuable information about the potential of a stress-tolerant product for marketable yield stability without yield drag.…”

Section: How Can Laboratory Stress Research Be Applied To Continuousl...mentioning

confidence: 99%

Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress

et al. 2022

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We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these questions include the need to better understand how plants detect water availability, temperature, salinity, and rising CO2 levels; how environmental signals interface with endogenous signaling and development (e.g. circadian clock, flowering time); and how this integrated signaling controls downstream responses (e.g. stomatal regulation, proline metabolism, growth versus defense balance). The plasma membrane comes up frequently as a site of key signaling and transport events (e.g. mechanosensing and lipid-derived signaling, aquaporins). Adaptation to water extremes and rising CO2 affects hydraulic architecture and transpiration, as well as root and shoot growth and morphology, in ways not fully understood. Environmental adaptation involves tradeoffs that limit ecological distribution and crop resilience in the face of changing and increasingly unpredictable environments. Exploration of plant diversity within and among species can help us know which of these tradeoffs represent fundamental limits and which ones can be circumvented by bringing new trait combinations together. Better defining what constitutes beneficial stress resistance in different contexts and making connections between genes and phenotypes, and between laboratory and field observations, are overarching challenges.

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Section: How Can Laboratory Stress Research Be Applied To Continuousl...mentioning

confidence: 99%

Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress

et al. 2022

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Tetrazolium viability test method for micro-seeds of the endangered plant Deinanthe caerulea

Xu,

Xiao,

et al. 2024

Seed Science and Technology

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Deinanthe caerulea is a rare perennial herb endemic to China that has high medicinal and ornamental value. Seeds of D. caerulea are tiny with a thousand seed weight of only 200 mg. Thus, classical germination tests are difficult to apply in this species and a rapid and accurate method for determining seed viability becomes an important prerequisite for species conservation, introduction and domestication. When using the tetrazolium (TTC) topographical staining method, removing the seed coat for TTC penetration is not practical. Therefore, in this experiment, we tried a hypochlorite seed treatment before the TTC test for discolouring and improving permeability of the seed coat. For three different seed samples, we found that treatment with 1% sodium chlorite solution for 30 minutes was the best pre-treatment, followed by staining with a 0.5% TTC solution for five hours. Staining patterns of embryos can then be observed under a microscope without any further preparation of the seeds. Only completely stained embryos were classified as viable. Simultaneously, germination tests were performed at different temperature and light regimes. The best regime was alternating 25/15°C and constant light at 3000 lx. Average germination percentage of the three samples was 79.75%, whereas viability was 86.77%. The results showed that a hypochlorite treatment before TTC staining is a versatile method for improving practicability of viability testing in Deinanthe caerulea.

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Exploring the High Variability of Vegetative Desiccation Tolerance in Pteridophytes

Alejo-Jacuinde

Herrera-Estrella

2022

Plants

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In the context of plant evolution, pteridophytes, which is comprised of lycophytes and ferns, occupy an intermediate position between bryophytes and seed plants, sharing characteristics with both groups. Pteridophytes is a highly diverse group of plant species that occupy a wide range of habitats including ecosystems with extreme climatic conditions. There is a significant number of pteridophytes that can tolerate desiccation by temporarily arresting their metabolism in the dry state and reactivating it upon rehydration. Desiccation-tolerant pteridophytes exhibit a strategy that appears to be intermediate between the constitutive and inducible desiccation tolerance (DT) mechanisms observed in bryophytes and angiosperms, respectively. In this review, we first describe the incidence and anatomical diversity of desiccation-tolerant pteridophytes and discuss recent advances on the origin of DT in vascular plants. Then, we summarize the highly diverse adaptations and mechanisms exhibited by this group and describe how some of these plants could exhibit tolerance to multiple types of abiotic stress. Research on the evolution and regulation of DT in different lineages is crucial to understand how plants have adapted to extreme environments. Thus, in the current scenario of climate change, the knowledge of the whole landscape of DT strategies is of vital importance as a potential basis to improve plant abiotic stress tolerance.

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Viability markers for determination of desiccation tolerance and critical stages during dehydration in Selaginella species

Cited by 3 publications

References 45 publications

Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress

Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress

Tetrazolium viability test method for micro-seeds of the endangered plant Deinanthe caerulea

Exploring the High Variability of Vegetative Desiccation Tolerance in Pteridophytes

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