Viability assessment of spermatozoa in large falcons (<i>Falco</i> spp.) using various staining protocols

Fischer, Dominik; Schneider, Helena; Failing, Klaus; Meinecke-Tillmann, S.; Wehrend, Axel; Lierz, Michael

doi:10.1111/rda.13785

Cited by 11 publications

(15 citation statements)

References 44 publications

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“…Eosin blue appeared as the most usable live/dead stain in falcons. In a recent study, the conventional stain eosin blue 2% enabled an efficient viability assessment, as well as SYBR ® 14/Green–propidium iodide (Merck KGaA, Darmstadt, Germany), showing significant and strong correlations with sperm motility [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although several authors have described the normal falcon sperm morphology [ 25 , 34 ], limited information is available on the percentages and types of sperm defects observed in raptors. In addition, no uniform system for classifying the sperm abnormalities has been proposed in avian species, which makes it difficult to perform comparisons.…”

Section: Discussionmentioning

confidence: 99%

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Morphological Characteristics of the Sperm of the Peregrine Falcon (Falco peregrinus) during the Reproductive Season

Oliveira

Martínez

Gil

et al. 2021

Veterinary Sciences

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The morphological characteristics of different sperm cells (normal, abnormal, and immature) in the peregrine falcon during the reproductive season were analysed. We also classified the main sperm defects found in semen. Semen samples were collected from mature peregrine falcons via cloacal massage and stained with Diff-Quik stain. The percentages of normal, abnormal, and immature sperm cells were determined by bright-field optical microscopy. The number of normal spermatozoa were greater at the initial stage and subsequently decreased during the middle and later stages of the reproductive season (p < 0.01). In contrast, the percentage of abnormal spermatozoa increased significantly in the middle and end stages of the reproductive season (p < 0.05), whereas the proportion of immature spermatozoa remained stable during the study. Head defects represented the greatest proportion of morphological abnormalities, followed by the defects in the tail and midpiece regions. A small percentage of multiple defects and cytoplasmic droplets were also observed in the falcon spermatozoa. The findings of this study might be important for the development of future conservation protocols for falcon sperm.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Morphological Characteristics of the Sperm of the Peregrine Falcon (Falco peregrinus) during the Reproductive Season

Oliveira

Martínez

Gil

et al. 2021

Veterinary Sciences

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“…Semen collected from falcon immediately tested in the slide one drop and stain sample by Eosin nigrosin stain and put cover slide , with subjected to microscopical examination (7) , at 40X.…”

Section: Dead and Live Spermmentioning

confidence: 99%

Comparative study of semen physical characteristics and DNA sperm integrity in different breeds of falcon in Iraq

Madhi

Hatif

2022

ijhs

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This study was conducted to evaluation of semen quality with the DNA integrity in the season of falcon in Iraq in the different breed (Saker falcon Falco peregrines, and Falco tinnunculus) due to selection of breeding falcons used in the reproduction .the objective of study was to characterize semen quality in the deferent species of falcon. A total of 24 semen sample were collected from males by massage method. Semen parameters include mass activity, individual motility, dead and alive, abnormality and DNA integrity. Mass activity varied between breeds mass activity observed significant (P≤0.05) in the middle period of season, individual motility shows significant between initial, middle and later of season (P≤0.05). The evaluation of viability show no significant between breeds. Some teratospermia recorded, micro-head, tailless, and immature sperms in the different breeds with no significant differences. DNA integrity revealed significant among the individual males (P≤0.05). In conclusion the season of reproduction in Iraq play a role of semen viability and quality between different falcon breeds in Iraq.

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“…Viability of sperms were analysed using EOSIN B 2% which stains dead cells in pink as described elsewhere (Fischer et al, 2020). In brief, staining solution and the diluted sample were mixed for 30 seconds on a slide and a thin smear was prepared at room temperature.…”

Section: Semen Analysis 1) Sperm Viabilitymentioning

confidence: 99%

Identification of sperm motility subpopulations in Gyr falcon (Falco rusticolus) ejaculate: a tool for investigating between subject variation

Seyedasgari¹,

Asadi²,

Sebastyen

et al. 2022

J Anim Reprod Biotechnol

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Artificial insemination technique (AI) forms the core of all falcon breeding programs which are intended to alleviate the pressure on wild populations since the adult birds of this species hardly copulate under captive situation (Bailey and Lierz, 2017). The outcome of falcon AI plans largely depends on semen characteristics in terms of quality and quantity (Parks and Hardaswick, 1987) which are often measured by routine laboratory techniques. Accurate assessment of avian semen achieved by Computer Assisted Sperm Analysis (CASA) as compared to conven-

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Viability assessment of spermatozoa in large falcons (Falco spp.) using various staining protocols

Cited by 11 publications

References 44 publications

Morphological Characteristics of the Sperm of the Peregrine Falcon (Falco peregrinus) during the Reproductive Season

Morphological Characteristics of the Sperm of the Peregrine Falcon (Falco peregrinus) during the Reproductive Season

Comparative study of semen physical characteristics and DNA sperm integrity in different breeds of falcon in Iraq

Identification of sperm motility subpopulations in Gyr falcon (Falco rusticolus) ejaculate: a tool for investigating between subject variation

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