Gotu Kola, scientifically known as Centella asiatica, has a long history of use as traditional medicine due to its numerous health benefits, which include anti-inflammatory, antioxidant effects, and wound healing. In this study, the researchers aimed to produce silver nanoparticles (AgNPs) using a green synthesis method that utilized C. asiatica extract. This method is a more eco-friendly alternative to conventional chemical synthesis methods. The AgNPs produced were analyzed using various techniques such as UV-visible spectrophotometry, XRD, FT-IR, SEM, and TEM to determine their size, shape, and structure. We observed that the AgNPs had a size of 390 nm, were crystalline in nature, had a FT-IR peak at 3437cm -1 , and had a size of 37±0.75 nm and 34±0.64 nm based on SEM and TEM analysis, respectively. Furthermore, the liquid-liquid extraction (LLE) method was used to extract various compounds from C. asiatica, including saponin, phenolic compounds, flavonoids, terpenoids, steroids, glycoside, and alkaloids. The extraction process resulted in different layers that contained phenolic compounds. The researchers found that the green synthesized AgNPs had better radical scavenging activity at their highest concentration, with an IC50 value of 385.364 µg/ml. Moreover, the minimum inhibitory concentration (MIC) of C. asiatica AgNPs on A549 lung cancer cells was found to be 216.972 µg/ml, indicating their potential as natural anticancer agents. Overall, this study highlights the potential of C. asiatica extract and its green synthesized AgNPs as natural remedies for cancer treatment. The green synthesis method used to produce AgNPs is also a promising approach to developing environmentally friendly methods for nanoparticle synthesis.