Viability and morphological evaluation of alginate-encapsulated Lactobacillus rhamnosus GG under simulated tilapia gastrointestinal conditions and its effect on growth performance, intestinal morphology and protection against Streptococcus agalactiae

Pirarat, Nopadon; Pinpimai, Komkiew; Rodkhum, Channarong; Chansue, Nantarika; Ooi, Ei Lin; Katagiri, Takayuki; Maita, Masashi

doi:10.1016/j.anifeedsci.2015.03.002

Cited by 39 publications

(17 citation statements)

References 44 publications

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“…In addition to vaccines, many functional feed additives have been proved to protect fish and shellfish against S. agalactiae such as Ku shen ( Sophora flavescens ) root extract (Wu et al, 2013 ), liposome-encapsulated cinnamaldehyde (Faikoh et al, 2014 ), B. subtilis and B. pumilus Ng et al, 2014 ; Liu H. et al, 2017 ; Srisapoome and Areechon, 2017 , yeast ( Saccharomyces cerevisiae ) (Pinpimai et al, 2015 ), Lb. rhamnosus (Pirarat et al, 2015 ), essential oils (Brum et al, 2017 ), buta–buta ( Excoecaria agallocha ) leaf extracts (Laith et al, 2017 ), β-glucan (Pilarski et al, 2017 ), kefir, low molecular weight sodium alginate, and Lb. plantarum Van Doan et al, 2016a , b , 2017b , and scarlet caterpillar ( Cordyceps militaris ) spent mushroom substrate and Lb.…”

Section: Pathogenic Labmentioning

confidence: 99%

Lactic Acid Bacteria in Finfish—An Update

et al. 2018

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A complex and dynamic community of microorganisms, play important roles within the fish gastrointestinal (GI) tract. Of the bacteria colonizing the GI tract, are lactic acid bacteria (LAB) generally considered as favorable microorganism due to their abilities to stimulating host GI development, digestive function, mucosal tolerance, stimulating immune response, and improved disease resistance. In early finfish studies, were culture-dependent methods used to enumerate bacterial population levels within the GI tract. However, due to limitations by using culture methods, culture-independent techniques have been used during the last decade. These investigations have revealed the presence of Lactobacillus, Lactococcus, Leuconostoc, Enterococcus, Streptococcus, Carnobacterium, Weissella, and Pediococcus as indigenous species. Numerous strains of LAB isolated from finfish are able to produce antibacterial substances toward different potential fish pathogenic bacteria as well as human pathogens. LAB are revealed be the most promising bacterial genera as probiotic in aquaculture. During the decade numerous investigations are performed on evaluation of probiotic properties of different genus and species of LAB. Except limited contradictory reports, most of administered strains displayed beneficial effects on both, growth—and reproductive performance, immune responses and disease resistance of finfish. This eventually led to industrial scale up and introduction LAB-based commercial probiotics. Pathogenic LAB belonging to the genera Streptococcus, Enterococcus, Lactobacillus, Carnobacterium, and Lactococcus have been detected from ascites, kidney, liver, heart, and spleen of several finfish species. These pathogenic bacteria will be addressed in present review which includes their impacts on finfish aquaculture, possible routes for treatment. Finfish share many common structures and functions of the immune system with warm-blooded animals, although apparent differences exist. This similarity in the immune system may result in many shared LAB effects between finfish and land animals. LAB-fed fish show an increase in innate immune activities leading to disease resistances: neutrophil activity, lysozyme secretion, phagocytosis, and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α). However, some LAB strains preferentially induces IL-10 instead, a potent anti-inflammatory cytokine. These results indicate that LAB may vary in their immunological effects depending on the species and hosts. So far, the immunological studies using LAB have been focused on their effects on innate immunity. However, these studies need to be further extended by investigating their involvement in the modulation of adaptive immunity. The present review paper focuses on recent findings in the field of isolation and detection of LAB, their administration as probiotic in aquaculture and their interaction with fish immune responses. Furthermore, the mode of action of probiotics on finfish are discussed.

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Section: Pathogenic Labmentioning

confidence: 99%

Lactic Acid Bacteria in Finfish—An Update

et al. 2018

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“…In addition, Lactobacillus can inhibit the growth of Enterobacteriaceae [ 51 ] and Streptococcus spp. [ 52 ]. Bifidobacterium is often detected in water as well as in the digestive tracts of fish [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Differences in the digestive enzyme activity, intestinal mucosa and microbial community in loach cultivated in two separate environments

Yang

Duan

et al. 2018

BMC Microbiol

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BackgroundFish culture in rice paddies can contribute to increasing yields of rice and surplus fish products. Environmental impacts and food-safety issues have become important topics in aquaculture, and organic foods currently were paid attention by researchers and industry practitioners. But the mechanism of differences in quality of Loach (Paramisgurnus dabryanus) reared in rice fields and ponds remains largely uncharacterized. In this study,digestive enzyme activity, intestinal mucosa cells and the gut microbial community of loach were determined under the two separate cultivation modes.ResultsThe levels of intestinal digestive enzyme activity of fish reared in the paddy-cultivated mode (PACM) were higher (P < 0.05) than those in the pond-cultivated mode (POCM). It was extremely significant (P < 0.01) for the activity of lipase in the liver, foregut and midgut, and for the activities of amylase and trypsin in the hindgut. Acid mucous cells in the loach foregut in PACM were fewer than in POCM (P < 0.01). In summer, the abundance of the Firmicutes, Lactobacillus spp., Aeromonas hydrophila, Enterobacteriaceae and Streptococcus spp. in loach intestinal mucosa in PACM was higher than in POCM. In fall, the abundance of total bacteria, the Bacteroidetes, Bifidobacterium and Enterobacteriaceae in the intestinal mucosa in PACM was likewise higher than in POCM. These differences were significant (P < 0.05 or P < 0.01) between loach in the two separate culture modes for all microorganisms except for A. hydrophila and Streptococcus spp. In addition, quantitative PCR assays showed that some microorganisms presented consistently similar abundances in the gut as in the culture water.ConclusionsThese results showed some enzymatic activities involved in digestion in liver and intestine of loach in PACM were higher than those in POCM, as using digestive enzyme analysis and histological observation of intestinal sections. These findings suggest most of the microorganisms examined in the gut mucosa of loach in the two culture modes significantly differed in abundance between summer and fall. However, some pathogenic bacteria in the gut, particularly A. hydrophila, presented lower abundance in PACM in fall, yet did not differ in abundance between loach in the two cultivation modes.

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“…However, a human‐derived probiotic has also shown to be suitable for fish (Pirarat et al . ). Many studies have reported that probiotics effectively increase the growth rate and immunity and improve the general health and survival rate of aquatic species (Irianto & Austin ; Hadi et al .…”

Section: Applications Of Encapsulation Technology In Aquaculture – Camentioning

confidence: 99%

“…An improvement in growth performance and protection against bacterial challenge was observed in tilapia fed with both alginate and skim milk–alginate encapsulates (Pirarat et al . ). However, skim milk–alginate capsules were reported to provide a significantly better protection after freeze drying and storage at room temperature compared to alginate alone (Pinpimai et al .…”

Section: Applications Of Encapsulation Technology In Aquaculture – Camentioning

confidence: 99%

“…; Pirarat et al . ) have been used successfully to deliver plasmid DNA, bacterin and probiotics to aquatic species, such as fish. Research by Winkel and Davids () has shown that the particle size selection by the mantle cavity and stomach was within the range of 15–50 μm with smaller and bigger sized particles being rejected and excreted in zebra mussel pseudo‐faeces.…”

Section: Bioactives Delivery In Aquaculture: Practical Considerationsmentioning

confidence: 99%

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Encapsulation for delivering bioactives in aquaculture

Dezfooli

Gutierrez‐Maddox

Seyfoddin

2018

Reviews in Aquaculture

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The exploitation of wild fisheries has driven the seafood industry to develop more sustainable resources and high-intensity aquaculture. However, high-density aquaculture is prone to outbreaks of diseases. The use of chemicals and antimicrobial drugs in aquaculture is common practice, but such chemicals can have serious detrimental environmental impacts and antibiotics may result in bacterial resistance in many species. The development of non-antibiotic and green strategies, such as using probiotics and immunostimulants, aims for a more sustainable practice for health maintenance in aquaculture. Although the efficacy of such strategies has been demonstrated in cultivations settings, the effective delivery of bioactives remains a challenge. Conventional delivery methods are often ineffective in terms of dosage, stability and species specificity, and can possibly cause widespread environmental contamination. However, recently developed drug delivery systems may provide innovative ways to improve delivery with minimal waste and improved environmental protection. Indeed, such cutting-edge drug delivery systems have already provided measurable benefits in human medicine over the past two decades. New technologies, such as encapsulation and controlled release systems, can be used readily in scaled-up operations to improve the delivery of bioactives and ultimately increase production and profitability in aquaculture. This review critically analyses the use of encapsulation technology with a focus on microparticles for delivery of bioactive agents to farmed aquatic animals. This review discusses various potential encapsulation materials, their properties and a range of methods that can be applied and scaled-up for aquaculture. Finally, practical considerations for designing an efficient delivery system for aquaculture are discussed.

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Viability and morphological evaluation of alginate-encapsulated Lactobacillus rhamnosus GG under simulated tilapia gastrointestinal conditions and its effect on growth performance, intestinal morphology and protection against Streptococcus agalactiae

Cited by 39 publications

References 44 publications

Lactic Acid Bacteria in Finfish—An Update

Lactic Acid Bacteria in Finfish—An Update

Differences in the digestive enzyme activity, intestinal mucosa and microbial community in loach cultivated in two separate environments

Encapsulation for delivering bioactives in aquaculture

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