Viability and genetic stability of pineapple germplasm after 10 years of in vitro conservation

Silva, Ronilze Leite da; Ferreira, Cláudia Fortes; Lêdo, Carlos Alberto da Silva; Souza, Everton Hilo de; Silva, Paulo Henrique da; Costa, Mirco; Souza, Fernanda Vidigal Duarte

doi:10.1007/s11240-016-1035-0

Cited by 45 publications

(40 citation statements)

References 35 publications

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“…A successful in vitro conservation program requires the development of regeneration strategies such as micropropagation (Bonilla et al, 2015;da Silva et al, 2016). TDZ is a phenylurea used as growth regulator for a rapid and effective in vitro regeneration of plant species (Ali et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…In vitro systems for midterm conservation offer the advantage of saving resources such as materials, space, and labor, in addition to preserving the plant material for an undetermined period of time through subculturing (da Silva et al, 2016;Pacheco et al, 2016). An option to reduce the in vitro development rate consists in reducing the concentrations of MS salts to minimize the source of nutrients (Mora et al, 2011;Pacheco et al, 2016), the use of growth retardants such as ancymidol (El-Dawayati et al, 2012;Sarkar et al, 2001), and osmoregulators as polyethylene glycol (PEG-800) (Seesangboon et al, 2018).…”

mentioning

confidence: 99%

“…However, there is no a study of in vitro preservation in the medium term to ensure regeneration of preserved material. However, establishing the conservation strategy for a particular species requires the development of an in vitro regeneration system (Bonilla et al, 2015;da Silva et al, 2016).…”

mentioning

confidence: 99%

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In Vitro Techniques to the Conservation and Plant Regeneration of Malanga (Colocasia esculenta L. Schott)

Mancilla-Álvarez¹,

Ramírez-Mosqueda²,

Arano-Avalos³

et al. 2019

horts

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Malanga (Colocasia esculenta) is a plant genetic resource that requires biotechnological strategies for conservation and propagation. One time-, labor-, and space-saving option is in vitro conservation and regeneration. The objective of this study was to develop a protocol for in vitro regeneration and conservation of germplasm of C. esculenta var. criolla. For conservation through minimal growth, we assessed several concentrations of Murashige and Skoog (MS) medium (one-third, one-half, and three-quarter strength), the growth retardant ancymidol (0, 1, 2, and 3 mg·L−1), and the osmoregulator polyethylene glycol (PEG-8000 mw) at different concentrations (0, 10, 20, and 30 g·L−1). For in vitro conservation, the percent survival, shoot number and length, and number of leaves and roots per explant were evaluated after 24 weeks. For in vitro regeneration, different concentrations of thidiazuron (TDZ: 0, 0.5, 1, 1.5, and 2 mg·L−1) and 6-benzylaminopurine (BAP; 0, 1, 2, 3, and 4 mg·L−1) were evaluated. After 4 weeks of cultivation, the percent response, shoot number, and number of leaves per explant were recorded. During in vitro conservation, it was noted that the treatment including 2 mg·L−1 ancymidol resulted in a retarded development, without affecting the survival of the C. esculenta germplasm. With regard to shoot regeneration, 7.60 shoots per explant were obtained using 2 mg·L−1 TDZ. Finally, 98% survival was achieved during the acclimatization process. This study will contribute to the establishment of genetic improvement programs through in vitro conservation and propagation of this valuable plant genetic resource.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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In Vitro Techniques to the Conservation and Plant Regeneration of Malanga (Colocasia esculenta L. Schott)

Mancilla-Álvarez¹,

Ramírez-Mosqueda²,

Arano-Avalos³

et al. 2019

horts

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“…Micropropagation protocols of pineapple have been developed and adjusted since the work of Aghion & Beauchesne (1960). From then many studies have demonstrated the applicability of this technique to the programs of pineapple genetic improvement, as well as for commercial production of plantlets (BE & DEBERGH, 2006;SOUZA et al, 2009;BARBOZA et al, 2009;AL-SAIF et al, 2011;ZURAIDA et al, 2011; SCHERER USMAN et al, 2013;FARAHANI, 2014;NELSON et al, 2015;SCHERER et al, 2015ab;OLIVEIRA-CAUDURO et al, 2016;SILVA et al, 2016;MENDONÇA, et al, 2017). C u r r e n t l y, c o n v e n t i o n a l t e c h n i q u e s o f micropropagation have been routinely used in the process of multiplication of new hybrids by research institutions, even though the protocols must be adjusted for each new variety.…”

Section: By Micropropagationmentioning

confidence: 99%

“…In micropropagation of pineapple, the genotype has a strong influence. Silva et al (2016) evaluated the viability and propagation potential of 66 genotypes of four botanical varieties: 44 genotypes of A. comosus var.…”

Section: By Micropropagationmentioning

confidence: 99%

Advances in pineapple plant propagation

et al. 2018

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Pineapple is one of the most important fruits, with large production in tropical and subtropical regions and great appreciation by consumers all over the world. The pineapple plant has many specific morphological, anatomical and physiological characteristics that determine crucial aspects of pineapple crop management, such as flower induction, water use and vegetative methods of propagation. The use of sexual reproduction of pineapple is restricted to breeding purposes carried out by research institutes looking for new hybrids with improved agronomic characteristics. Seeds are only produced if cross pollination among varieties occurs. Commercially pineapple has to be propagated by vegetative material, an asexual reproduction, without new combinations of genes. Some types of propagules are naturally produced by the plants and called conventional planting material. Its availability and quality depend on many factors, especially cultivar and environment. Management techniques of this material have been continuously developed and will be addressed. In addition to the conventional planting material, which in many situations is not sufficient to assure expansion or at least maintenance of the cultivated area, several other methods of vegetative propagation of pineapple have been studied and made available along the last decades and will also be discussed, involving techniques of stem sectioning, apical growing point gouging and chemical treatment for transformation of flowers into plantlets. Stem sectioning has been especially interesting, as it is mostly done using plant residues available at low cost, and is a rather simple method suited for multiplication and production of disease-free planting material in nurseries. Gouging and chemical treatment are less practiced, but can be applied in ratoon crops, thereby avoiding the loss of the first cycle fruit. Chemical treatment usually results in rather small plantlets, that must be further grown in nurseries before planting them in the field. And finally micropropagation will also be focused, as in vitro production of plantlets is a very important method of multiplication of new pineapple varieties, but this method yet has not been transformed into a common commercial way of pineapple propagation due to the final high cost and to the still high risks of incidence of somaclonal variations among the plantlets produced.

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Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique

Souza

Kaya

et al. 2018

Methods in Molecular Biology

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Cryopreservation is a technique that allows the conservation of many species for long periods. Among the protocols used for cryopreservation, droplet vitrification has shown efficient results in preserving shoot tips of various wild and cultivated pineapple genotypes. The method consists of extraction of shoot tips from plants grown in vitro, dehydration for a period of 48 h in a preculture medium supplemented with a high concentration of sucrose, treatment in a plant vitrification solution (PVS2), and immersion in liquid nitrogen. The method described in this chapter has produced survival and regeneration indices of around 70%, depending on the genotype and physiological conditions of the initial explants. The objective of this chapter is to describe in detail a droplet vitrification protocol for shoot tips that is easy to perform for cryopreservation of pineapple germplasm.

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Viability and genetic stability of pineapple germplasm after 10 years of in vitro conservation

Cited by 45 publications

References 35 publications

In Vitro Techniques to the Conservation and Plant Regeneration of Malanga (Colocasia esculenta L. Schott)

In Vitro Techniques to the Conservation and Plant Regeneration of Malanga (Colocasia esculenta L. Schott)

Advances in pineapple plant propagation

Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique

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