Viabilidad de espermatozoides ovinos mantenidos a 5º y 15ºC en diferentes sistemas de refrigeración

Moscardini, Murillo Mansano; Scott, Caroline; Moura, Diego Souza; Lourenço, Tarcísio Torre; Aristizábal, Viviana Helena Vallejo; Souza, Fabiana Ferreira de

doi:10.4322/rbcv.2014.035

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Another practical system for the transport of diluted semen that is also used in the field is Botutainer® (Botupharma, Botucatu, Brazil). According to the manufacturer, this system maintains the semen temperature at 5°C for up to 24 h. This transport box for cooled semen have been reported to be efficient in maintaining semen viable after cooling (Monteiro et al, 2013;Crespilho et al, 2014;Moscardini et al, 2014;Borges-Silva et al, 2016). Recently, programmable units for semen cryopreservation have been developed, which permit the selection of the desired cooling and freezing curve.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cooling and freezing systems of bovine semen

Dias

Campanholi

Rossi

et al. 2018

Animal Reproduction Science

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Semen cryopreservation comprises different steps, among them are the cooling and freezing rates which significantly influence the quality of thawed sperm. Different systems with variable freezing rates are used for freezing bull semen in the field, with a consequence of variable success rates. The objective of this study was to compare different systems for freezing bull semen in the field. Five cooling methods of semen and two methods for the subsequent freezing phase (5 × 2 factorial scheme) were used. Two to four ejaculates were collected from 12 bulls with an electroejaculator. The ejaculates were diluted in BotuBov® to a concentration of 50 × 10 6 spermatozoa/mL in 0.5-mL straws. After dilution, the straws were cooled to 5°C in five cooling systems: TK 4000® at a cooling rate of −0.25°C/min (R1); TK 4000® at a rate of −0.5°C/min (R2); Minitube® refrigerator at a rate of −2.8°C/min (R3); Botutainer® at a rate of −0.65°C (R4), and domestic refrigerator at a rate of −2.0°C/min (R5). After stabilization at 5°C for 4 h, these straws were then submitted to two freezing systems: TK 4000® at a freezing rate of −15°C/min (C1) and Styrofoam box with liquid nitrogen at a rate of −19°C/min (C2). Sperm kinetics were evaluated by computer-assisted sperm analysis at four time points: in fresh semen, after cooling, post-thawing, and after the rapid thermal resistance test (TRT). In addition, plasma and acrosomal membrane integrity, mitochondrial potential and intracellular H 2 O 2 were analyzed after thawing by flow cytometry. The R1, R2 and R4 cooling systems were the most efficient in preserving sperm viability, membrane integrity and intracellular H 2 O 2. Samples frozen in the C1 system exhibited better post-thaw and post-TRT kinetics than C2 samples. In conclusion, slower cooling curves in conjunction with a constant freezing rate obtained with the programmable unit were more efficient for freezing bull semen in the field.

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Section: Introductionmentioning

confidence: 99%

Evaluation of cooling and freezing systems of bovine semen

Dias

Campanholi

Rossi

et al. 2018

Animal Reproduction Science

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“…The Botutainer ® [ 12 ] is a commonly used transportation device in Brazil that has an initial cooling curve of approximately −0.3 °C/min, guaranteeing a final storage temperature of around 5 °C and a maximum storage time of 48 h [ 12 ]. The application of transport devices aims to ensure a slow decline in temperature to minimize cold damage to spermatozoa [ 13 , 14 ]. The ability to transport cooling sperm from free-living species allows genetic material to be exchanged between institutions (zoos, reserves, breeding grounds, etc.)…”

Section: Introductionmentioning

confidence: 99%

Effect of Diluents and Storage Time on the Cryopreservation of Collared Peccary (Pecari tajacu) Semen after Cooling Storage in a Transport Container at 5 °C

Santos,

Silva,

Pereira

et al. 2024

Animals

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We verified the possibility of cooling peccary semen for 4, 24, and 48 h before cryopreservation, using different dilution media (TRIS + egg yolk (20%) and PRIMXcell Ultra). Ten ejaculates were divided equally into six aliquots and then diluted. Two aliquots were stored in a biological incubator (4 h), and the remaining aliquots were stored in a commercial container, the Botutainer® (24 and 48 h), both at 5 °C. The samples were cryopreserved and then evaluated for kinetic parameters, functionality, integrity, mitochondrial activity, morphology, and sperm binding capacity. After thawing, samples diluted in TRIS showed total motility of 43.4 ± 6.8%, 48.4 ± 6.2%, and 38.6 ± 5.0% after cooling for 4, 24, and 48 h before cryopreservation, respectively. Such results are significantly greater than those achieved with the use of PRIMXcell diluent for 4 (8.3 ± 2.8%), 24 (4.7 ± 1.4%), and 48 h (4.8 ± 2.9%) storage (p < 0.05). Furthermore, TRIS provided better preservation of sperm membrane integrity when samples were cooled for 24 h (44.5 ± 4.7%) before cryopreservation compared to those samples diluted in PRIMXcell Ultra stored for 24 (25.7 ± 4.0%) and 48 h (25.2 ± 4.0%) before freezing (p < 0.05). In summary, we suggest TRIS diluent + egg yolk (20%) as an effective option to allow semen to cool for 24 or 48 h in a transport container before cryopreservation.

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Viabilidad de espermatozoides ovinos mantenidos a 5º y 15ºC en diferentes sistemas de refrigeración

Cited by 2 publications

References 12 publications

Evaluation of cooling and freezing systems of bovine semen

Evaluation of cooling and freezing systems of bovine semen

Effect of Diluents and Storage Time on the Cryopreservation of Collared Peccary (Pecari tajacu) Semen after Cooling Storage in a Transport Container at 5 °C

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