VHSV G glycoprotein major determinants implicated in triggering the host type I IFN antiviral response as DNA vaccine molecular adjuvants

Martínez‐López, Alicia; García-Valtanen, Pablo; Ortega-Villaizán, M.; Chico, V.; Gómez-Casado, Eduardo; Coll, Julio; Estepa, A.

doi:10.1016/j.vaccine.2014.07.111

Cited by 29 publications

(24 citation statements)

References 51 publications

(59 reference statements)

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“…For intracellular staining, mouse polyclonal antibodies against rainbow trout IL1β (RRID: AB_2716269) 21 , 22 , IL8 (RRID: AB_2716272) 23 and TNF-α (RRID: AB_2716270) 24 were produced at the laboratory of Dr. Luis Mercado. Rabbit polyclonal antibody against rainbow trout Mx3 (RRID: ABA_2716267) 25 , 26 was produced at the laboratory of Dr. Amparo Estepa. Anti-IPNV-VP3 monoclonal antibody 2F12 (RRID: AB_2716296) was used for IPNV labelling 27 .…”

Section: Methodsmentioning

confidence: 99%

Infectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective

et al. 2017

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Background: Some fish viruses, such as piscine orthoreovirus and infectious salmon anemia virus, target red blood cells (RBCs), replicate inside them and induce an immune response. However, the roles of RBCs in the context of infectious pancreatic necrosis virus (IPNV) infection have not been studied yet. Methods: Ex vivo rainbow trout RBCs were obtained from peripheral blood, Ficoll purified and exposed to IPNV in order to analyze infectivity and immune response using RT-qPCR, immune fluorescence imaging, flow cytometry and western-blotting techniques. Results: IPNV could not infect RBCs; however, IPNV increased the expression of the INF1-related genes ifn-1, pkr and mx genes. Moreover, conditioned media from IPNV-exposed RBCs conferred protection against IPNV infection in CHSE-214 fish cell line. Conclusions: Despite not being infected, rainbow trout RBCs could respond to IPNV with increased expression of antiviral genes. Fish RBCs could be considered as mediators of the antiviral response and therefore targets of new strategies against fish viral infections. Further research is ongoing to completely understand the molecular mechanism that triggers this antiviral response in rainbow trout RBCs.

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Section: Methodsmentioning

confidence: 99%

Infectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective

et al. 2017

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“…On the other hand, cell were fixed with a solution of 4% formaldehyde (Sigma, F1635) for 15 min, washed with PBS and further fixed with cold methanol (−20°C) for 15 min. Fixed cells were stained with a monoclonal antibody to SVCV (Teknokroma Analítica S.A. monoclonal antibody anti-SVCV) at 4°C for 24h [ 45 ]. After washing with PBS and cell monolayers were incubated with a FITC-labelled rabbit anti-mouse antibody (SIGMA) diluted 1/500 and incubation was continued for 30 min.…”

Section: Methodsmentioning

confidence: 99%

TNFα Impairs Rhabdoviral Clearance by Inhibiting the Host Autophagic Antiviral Response

et al. 2016

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TNFα is a pleiotropic pro-inflammatory cytokine with a key role in the activation of the immune system to fight viral infections. Despite its antiviral role, a few viruses might utilize the host produced TNFα to their benefit. Some recent reports have shown that anti-TNFα therapies could be utilized to treat certain viral infections. However, the underlying mechanisms by which TNFα can favor virus replication have not been identified. Here, a rhabdoviral infection model in zebrafish allowed us to identify the mechanism of action by which Tnfa has a deleterious role for the host to combat certain viral infections. Our results demonstrate that Tnfa signals through its receptor Tnfr2 to enhance viral replication. Mechanistically, Tnfa does not affect viral adhesion and delivery from endosomes to the cytosol. In addition, the host interferon response was also unaffected by Tnfa levels. However, Tnfa blocks the host autophagic response, which is required for viral clearance. This mechanism of action provides new therapeutic targets for the treatment of SVCV-infected fish, and advances our understanding of the previously enigmatic deleterious role of TNFα in certain viral infections.

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“…Adjuvants elicit immune responses through different signaling pathways, and can improve vaccine formulations for better protection (Antúnez et al, 2016, Czajka et al, 2012, Aghasadeghi et al, 2011. However, as alum or oil adjuvants may cause local postvaccination reactions in animals, these widely used chemical adjuvants are often associated with several disadvantages (Martinez-Lopez et al, 2014, Liu et al, 2013. Therefore, it is crucial to develop novel adjuvants for poultry vaccines.…”

Section: Introductionmentioning

confidence: 99%

Adjuvant Effects of Platycodin D on Immune Responses to Infectious Bronchitis Vaccine in Chickens

Zhou

Mao

2020

J. Poult. Sci.

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Adjuvants are common vaccine components. Novel adjuvants may improve the protective immunity conferred by vaccines against poultry diseases. Here, a lesshemolytic saponin, platycodin D (PD), isolated from the root of Platycodon grandiflorum was investigated as a potential alternative adjuvant. PD was tested as an adjuvant in the infectious bronchitis (IB) vaccine, because the existing IB vaccine has often failed to induce effective immune responses. The adjuvant activity of PD in conjunction with IB vaccine was evaluated in this study. Compared to control treatment, PD treatment significantly increased the proliferation of chicken peripheral blood mononuclear cells, concentration of interferon-γ in culture supernatants, and anti-IB antibody titer. In chickens pre-challenged with the Mass 41 infectious bronchitis virus (IBV), PD administration resulted in fewer and less severe clinical signs, lower mortality rate, and higher protection compared to control treatment. Histopathological examination showed that the lungs and kidneys of PD-treated chickens displayed fewer pathological lesions than those of control chickens. Our results also demonstrated that this new vaccine adjuvant improved chicken humoral and cellular immune responses without any side effects. Hence, our findings suggest that PD might serve as an effective adjuvant in IBV vaccines.

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VHSV G glycoprotein major determinants implicated in triggering the host type I IFN antiviral response as DNA vaccine molecular adjuvants

Cited by 29 publications

References 51 publications

Infectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective

Infectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective

TNFα Impairs Rhabdoviral Clearance by Inhibiting the Host Autophagic Antiviral Response

Adjuvant Effects of Platycodin D on Immune Responses to Infectious Bronchitis Vaccine in Chickens

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