Vg1 RNA binding protein mediates the association of Vg1 RNA with microtubules in Xenopus oocytes.

Elisha, Zichrini; Havin, Leora; Ringel, Israel; Yisraeli, Joel K.

doi:10.1002/j.1460-2075.1995.tb00193.x

Cited by 94 publications

(61 citation statements)

References 35 publications

(43 reference statements)

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“…In Xenopus laevis, there is a single member of this family, termed Vg1 RBP (Havin et al 1998) or Vera (Deshler et al 1998), that was initially identified based on its ability to bind Vg1 RNA with high affinity. Vg1 RBP, or xVICKZ3 (according to the terminology suggested in Yaniv and Yisraeli 2002), is part of a complex of several RNA binding and motor proteins that help localize Vg1 RNA to the vegetal cortex of Xenopus oocytes in a mechanism requiring both intact microtubules and microfilaments (Yisraeli et al 1990;Elisha et al 1995;Cote et al 1999;Zhao et al 2001;Kroll et al 2002;Allison et al 2004;Betley et al 2004;Lewis et al 2004;Yoon and Mowry 2004;Czaplinski et al 2005;Czaplinski and Mattaj 2006). In Xenopus embryos, xVICKZ3 plays a role in single cell movements.…”

Section: Introductionmentioning

confidence: 99%

VICKZ proteins mediate cell migration via their RNA binding activity

Oberman¹,

Rand²,

Maizels³

et al. 2007

RNA

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The highly conserved, RNA binding VICKZ proteins help regulate RNA localization, stability, and translation in many eukaryotes. These proteins are also required for cell migration in embryos and cultured cells. In adults, many tumors overexpress VICKZ homologs, and it has been hypothesized that the proteins can mediate cell motility and invasion. How these proteins facilitate cell movement and, in particular, whether their ability to bind RNA plays a role in their function remain unclear. Using HPLC and mass spectrometry to identify a region of Xenopus Vg1 RBP (xVICKZ3) that binds the vegetal localization element of Vg1 RNA, we generated a deletion construct that functions in a dominant-negative manner. The construct associates with full-length xVICKZ3 and severely reduces binding to target RNAs. This dominant-negative construct phenocopies the effect of down-regulating xVICKZ3 in Xenopus embryos. A corresponding deletion in the human homolog hVICKZ1 similarly functions in a dominant-negative fashion to reduce the ability of full-length hVICKZ protein to bind RNA. Expression of the dominant-negative construct in human carcinoma cells inhibits cell movement by several criteria. We conclude that the ability of VICKZ proteins to mediate cell migration, in vitro and in vivo, requires their RNA binding activity.

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Section: Introductionmentioning

confidence: 99%

VICKZ proteins mediate cell migration via their RNA binding activity

Oberman¹,

Rand²,

Maizels³

et al. 2007

RNA

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“…Vg1 RBP (also known as Vera) is one such RNA-binding protein that has been implicated in the intracellular localization of Vg1 RNA to the vegetal cortex of Xenopus oocytes (Havin et al, 1998;Deshler et al, 1998). Vg1 RBP recognizes cis-acting elements in the 3′ UTR of Vg1 RNA (Bubunenko et al, 2002;Deshler et al, 1998;Deshler et al, 1997;Havin et al, 1998;Kwon et al, 2002;Mowry, 1996;Schwartz et al, 1992) and is likely to facilitate localization by mediating its association with microtubules in oocytes (Elisha et al, 1995). Vg1 RBP mRNA is also expressed in a number of cell types during embryogenesis, although the role of the protein in these different tissues has not been examined (Mueller-Pillasch et al, 1999;Zhang et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

The RNA-binding protein Vg1 RBP is required for cell migration during early neural development

et al. 2003

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After mid-blastula transition, populations of cells within the Xenopus embryo become motile. Using antisense morpholino oligonucleotides, we find that Vg1 RBP, an RNA-binding protein implicated in RNA localization in oocytes, is required for the migration of cells forming the roof plate of the neural tube and, subsequently, for neural crest migration. These cells are properly determined but remain at their site of origin. Consistent with a possible role in cell movement, Vg1 RBP asymmetrically localizes to extended processes in migrating neural crest cells. Given that Vg1 RBP is a member of the conserved VICKZ family of proteins, expressed in embryonic and neoplastic cells, these data shed light on the likely role of these RNA-binding proteins in regulating cell movements during both development and metastasis.

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“…The first, alternatively called Vg1 RBP or Vera, is a Xenopus homolog of chicken zipcodebinding protein, which binds to and determines localization of β-actin mRNA in fibroblasts (Deshler et al, 1998;Havin et al, 1998). Vg1 RBP/Vera appears to be necessary for the association of Vg1 mRNA with microtubules (Elisha et al, 1995) and is itself associated with a subcompartment of the endoplasmic reticulum (Deshler et al, 1997). A second factor, which is a homolog of hnRNP I, binds to and colocalizes with Vg1 mRNA (Cote et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

A homolog of FBP2/KSRP binds to localized mRNAs inXenopusoocytes

et al. 2002

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A Xenopus oocyte expression library was screened for proteins that bind to the 340-nucleotide localization element of Vg1 mRNA. Four different isolates encoded a Xenopus homolog of the human transcription factor, FUSE-binding protein 2 (FBP2). This protein has been independently identified as the splicing regulatory factor KSRP. The only significant difference between the Xenopus protein, designated VgRBP71, and KSRP is the absence of a 58 amino acid segment near the N-terminal of the former. In vivo binding assays show that VgRBP71 is associated with mRNAs localized to either the vegetal or animal hemispheres, but was not found with control mRNAs.Unlike other factors that bind to the localization element of Vg1 mRNA, VgRBP71 does not accumulate at the vegetal cortex with the mRNA; rather, it is present in the nucleus and throughout the cytoplasm at all stages of oogenesis. Cytoplasmic VgRBP71 appears to be most concentrated at the cell cortex. VgRBP71 interacts with Prrp, another protein that binds to the Vg1 localization element; this association does not require the presence of Vg1 mRNA.

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Vg1 RNA binding protein mediates the association of Vg1 RNA with microtubules in Xenopus oocytes.

Cited by 94 publications

References 35 publications

VICKZ proteins mediate cell migration via their RNA binding activity

VICKZ proteins mediate cell migration via their RNA binding activity

The RNA-binding protein Vg1 RBP is required for cell migration during early neural development

A homolog of FBP2/KSRP binds to localized mRNAs inXenopusoocytes

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