Veterinary Biomarker Discovery: Proteomic Analysis of Acute Phase Proteins

Boehmer, Jamie L.; Olumee-Shabon, Zohra

doi:10.5772/19263

Cited by 1 publication

(1 citation statement)

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“…Currently, the methods used for APP quantification are diverse and often based on techniques that use antibodies that are not necessarily pig specific. In addition, current protocols do not usually support the distinction between isoforms or the evaluation of post-translational modifications . Immunoenzymatic analysis methods may have specificity problems and can hardly be multiplexed because the antibodies' cross-reactivity cannot always be overcome, limiting assay performances and resulting in inaccurate results .…”

Section: Introductionmentioning

confidence: 99%

Multiplex Assay to Determine Acute Phase Proteins in Modified Live PRRSV Vaccinated Pigs

Tor,

Fraile,

Vilaró

et al. 2024

J. Proteome Res.

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Acute phase protein (APP) response to vaccine challenges is an attractive alternative to natural infection for identifying pigs with increased disease resilience and monitoring the productive performance. Currently, the methods used for APP quantification are diverse and often based on techniques that use antibodies that are not necessarily pig specific. The objective of this work is the development of a method based on a UPLC-SRM/ MS system for simultaneous determination of haptoglobin, apolipoprotein A1, C-reactive protein, pig-major acute protein, and serum amyloid A and its application in pigs to monitor the effect of a vaccine administered against porcine reproductive and respiratory syndrome virus (PRRSV). With the aim of tracing the complete analytical process for each proteotypic peptide, a synthetic QconCat polypeptide construct was designed. It was possible to develop an SRM method including haptoglobin, apolipoprotein A1, pig-MAP, and serum amyloid A1. The PRRSV vaccine only affected haptoglobin. The pigs with positive viremia tended to show higher values than negative pigs, reaching significant differences in the three haptoglobin SRMdetected peptides but not with the data acquired by immunoenzymatic and spectrophotometric assays. These results open the door to the use of SRM to accurately monitor APP changes in experimental pigs.

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Section: Introductionmentioning

confidence: 99%