Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins

Regente, Mariana; Corti-Monzón, Georgina; Maldonado, Antonio; Pinedo, Marcela; Jorrı́n, Jesús; Canal, Laura de la

doi:10.1016/j.febslet.2009.09.041

Cited by 181 publications

(169 citation statements)

References 32 publications

Supporting

Mentioning

169

Contrasting

Order By: Relevance

“…In fact, we have already detected the presence of exosome-like structures in apoplastic fluids of sunflower. 12 These particles, of 20-200 nm, have been shown to contain a Rab11A, typical proteins implicated in the production of vesicles from donor membranes and their fusion with acceptor ones. More important, we found that these vesicles were enriched in Helja, The existence of apoplastic vesicles in plants has also been reported in other experimental systems but no function has still been assigned to these structures.…”

Section: Apoplastic Vesicles and Proteins Without Predicted Signal Pementioning

confidence: 99%

See 1 more Smart Citation

Apoplastic exosome-like vesicles: A new way of protein secretion in plants?

Regente

Pinedo

Elizalde

et al. 2012

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

These authors contributed equally to this work.Keywords: apoplast, protein secretion, signal sequence, non-classical secretionThe presence of apoplastic proteins without predicted signal peptide in the gene sequence suggests the existence of protein secretion independent of the ER/Golgi classical route. In animals, one of the pathways proposed for alternative protein secretion involves the release of exosomes to the extracellular space. Although this pathway has not been dissected in plants some indirect evidence is emerging. We have reported that apoplastic fractions of sunflower seeds contain exosome-like vesicles. Besides, these vesicles are enriched in the lectin Helja, which is immunolocalized in the extracellular space even if it the protein has no predicted signal peptide. Here we show that Helja is not glycosylated and its secretion is insensitive to brefeldin A, two of the major characteristics to discard ER/Golgi-mediated protein transport. Moreover, the levels of Helja in sunflower extracellular vesicles are not affected by brefeldin A treatment. Our results suggest that Helja could be exported through an exosome-mediated pathway and point out that this mechanism may be responsible for the secretion of at least part of the leaderless proteins detected in the extracellular compartment of plants.

show abstract

Section: Apoplastic Vesicles and Proteins Without Predicted Signal Pementioning

confidence: 99%

“…Sunflower seeds were imbibed in water (Control) or 50 mg ml 21 brefeldin A (BFA) for 2 h and subjected to extracellular fluid (EF) extraction or extracellular vesicles preparation as previously described. 12 The EF …”

Section: An Extracellular Lectin Detected In Exosome-like Vesicles Fumentioning

confidence: 99%

Apoplastic exosome-like vesicles: A new way of protein secretion in plants?

Regente

Pinedo

Elizalde

et al. 2012

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

show abstract

“…17 The presence of exosome-like vesicles has been also reported in apoplastic fluids of sunflower seeds. 18 Exosomes contain a number of proteins, many of which are typically intracellular. 17,19 Interestingly, the olive stigma exudate shows a manifest vesicular aspect at anthesis, and contains numerous vesicle-like particles in the size range (40-100 nm) expected for exosomes.…”

Section: The Plant Stigma Exudatementioning

confidence: 99%

The plant stigma exudate

Rejón

Delalande

Schaeffer-Reiss

et al. 2014

Plant Signaling & Behavior

View full text Add to dashboard Cite

D uring sexual reproduction, pollenperformance is greatly influenced by the female tissues. The stigma exudate, i.e., the extracellular secretion that covers the stigma outermost surface, has been usually regarded as a reservoir of water, secondary metabolites, cell wall precursors and compounds that serve as energy supply for rapid pollen tube growth. In an attempt to identify the proteins present in the stigma secretome, we performed a large-scale analysis in two species (Lilium longiflorum and Olea europaea) following a proteomicbased approach. The resulting data strongly suggest that the stigma exudate is not a mere storage site but also a biochemically active environment with a markedly catabolic nature. Thus, this secretion may modulate early pollen tube growth and contribute to the senescence of stigma after pollination. In addition, a putative cross-talk between genetic programs that regulate stress/defense and pollination responses in the stigma is also suggested. The stigma exudate might also functionally diverge between species on the basis on their ecology and the biochemical, morphological and anatomical features of their stigmas. Unexpectedly, we identified in both exudates some intracellular proteins, suggesting that a mechanism other than the canonical ER-Golgi exocytic pathway may exist in the stigma and contribute to exudate secretion.During sexual reproduction, pollen performance is greatly influenced by the biochemical, morphological and anatomical features of the female counterpart (i.e., the pistil). In some plants, the stigma outermost surface is coated by a secretion that is released by specialized cells called papillae. This extracellular fluid, the so-called stigma exudate, mostly contains water, sugars, lipids and proteins, 1 but other compounds such as phenols, amino acids, ROS/ RNS and Ca 2+ ions are also present in measurable amounts. [2][3][4][5] The picture of the stigma secretome remains fragmentary and only a few proteins have been identified and characterized so far. [6][7][8] In a recent publication, we performed a largescale protein profiling study of the stigma exudate in two plant species, namely Eastern lily (Lilium longiflorum) and olive (Olea europaea), that substantially differ in their taxonomical position, pistil morphology and anatomy, ecology and cultivation purposes. 9 Following a proteomic-based strategy, we identified 51 and 57 unique proteins in lily and olive, respectively, most of which are first described in this secretion. The resulting data shows that the complexity of the stigma exudate, in terms of protein diversity, is higher than initially expected. 10 Under a functional perspective, this secretion has often been regarded as a mere source of water for pollen hydration, and storage compounds for sustaining

show abstract

“…Already exist publications that describe the release of exosomes from tubers like carrot or ginger [62], from nuts and seeds sunflower [63] or from fruits like grape, watermelon or lemon. It is known that grapefruit nanovesicles, isolated from grape juice, act as immune modulators in the intestine and are able to attenuate inflammatory response in human disease [64]; furthermore there are clinical trials focus on their possible application to prevent cancer progression.…”

Section: Extracellular Vesicles In Plantsmentioning

confidence: 99%

Natural Compounds: Molecular Weapons against Leukemia’s

Taverna¹,

Corrado

2017

J Leuk

View full text Add to dashboard Cite

Nowadays cancer is one of the main reasons of death all over the world and it is estimated that deaths caused by cancer will grow dramatically in the next decades. Even if chemotherapy is the election therapy for solid tumors, as well as leukemias and lymphomas, cancer treatments are in continuous evolution trying to solve the problem of resistance mainly due to low accumulation of the drug in tumor cells (MDR). Natural compounds represent a valid alternative to treat several disease and recently the scientific community focus on these natural compounds and plant metabolites with therapeutic activities and low toxicities compared with synthetic ones.A combination therapy, that join conventional chemotherapy with natural plant metabolites, is now considered a new promising strategy to overcome MDR and reduce cellular toxicity; in particular, in leukemia due to its very complex origin and development of leukemogenesis.Here, we want to summarize and update the recent applications of natural compounds in the treatment of leukemia.

show abstract

Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins

Cited by 181 publications

References 32 publications

Apoplastic exosome-like vesicles: A new way of protein secretion in plants?

Apoplastic exosome-like vesicles: A new way of protein secretion in plants?

The plant stigma exudate

Natural Compounds: Molecular Weapons against Leukemia’s

Contact Info

Product

Resources

About