Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

Grieshaber, Dorothee; Lange, Victoria de; Hirt, Thomas; Lu, Zhihua; Vörös, János

doi:10.3390/s8127894

Cited by 18 publications

(9 citation statements)

References 23 publications

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“…Fractions 4 and 5 exhibited the most pronounced dissipation of 14.7 ± 1.9 and 13.5 ± 1.6, on average, respectively ( Figure 3B). This phenomenon has been previously reported by groups sensing synthetic vesicles, 60,61 where the viscoelastic structures resulted in energy storage (elastic) and loss (viscous) during oscillation. This is of interest, as it provides another discriminating factor to determine whether bound adsorbates are exosomal (vesicular and dissipative) or artifacts (nonvesicular and rigid).…”

Section: Analytical Chemistrysupporting

confidence: 76%

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

Suthar¹,

Parsons²,

Hoogenboom³

et al. 2019

Preprint

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Exosomes are endocytic lipid-membrane bound bodies with potential to be used as biomarkers in cancer and neurodegenerative disease. The limitations and scarcity of current exosome characterisation approaches has led to a growing demand for translational techniques, capable of determining their molecular composition and physical properties in physiological fluids. Here, we investigate label-free immunosensing, using a quartz crystal microbalance with dissipation (QCM-D), to detect exosomes by exploiting their surface protein profile. Exosomes expressing the transmembrane protein CD63 were isolated by size-exclusion chromatography from cell culture media. QCM-D sensors functionalised with anti-CD63 antibodies formed a direct immunoassay towards CD63-positive exosomes, exhibiting a limit-of-detection of 1.7x10^8 and 1.1x10^8 exosome sized particles (ESPs) ml^-1 for frequency and dissipation response respectively, i.e., clinically relevant concentrations. Our proof-of-concept findings support the adoption of dual-mode acoustic analysis of exosomes, leveraging both frequency and dissipation monitoring for use in diagnostic assays.

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Section: Analytical Chemistrysupporting

confidence: 76%

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

Suthar¹,

Parsons²,

Hoogenboom³

et al. 2019

Preprint

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show abstract

“…The interaction of the biological detection element and the analyte is determined by the transducer unit. This unit can use optical [ 44 ], electrochemical [ 45 ], calorimetrical [ 46 ], or piezoelectrical principles [ 47 ]. The integration of biological elements such as enzymes, microorganisms, and antibodies as sensing materials makes the transducer selective and sensitive.…”

Section: State Of the Artmentioning

confidence: 99%

Towards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologies for bio-based production processes

Gargalo

Udugama

Pontius

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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The biomanufacturing industry has now the opportunity to upgrade its production processes to be in harmony with the latest industrial revolution. Technology creates capabilities that enable smart manufacturing while still complying with unfolding regulations. However, many biomanufacturing companies, especially in the biopharma sector, still have a long way to go to fully benefit from smart manufacturing as they first need to transition their current operations to an information-driven future. One of the most significant obstacles towards the implementation of smart biomanufacturing is the collection of large sets of relevant data. Therefore, in this work, we both summarize the advances that have been made to date with regards to the monitoring and control of bioprocesses, and highlight some of the key technologies that have the potential to contribute to gathering big data. Empowering the current biomanufacturing industry to transition to Industry 4.0 operations allows for improved productivity through information-driven automation, not only by developing infrastructure, but also by introducing more advanced monitoring and control strategies.

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“…QCM-D system can be used to characterize the formation of thin films from proteins, polymers and cells on surfaces (Höök and Kasemo, 2007). There have been relatively few efforts made to adapt QCM-D to biosensor development (Grieshaber et al, 2008;Poitras and Tufenkji, 2009). To the best of our knowledge, the amplification of Apt-GNPs with QCM-D detection has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles

Chen

Tang

Wang

et al. 2010

Biosensors and Bioelectronics

112

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Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

Cited by 18 publications

References 23 publications

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

Towards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologies for bio-based production processes

Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles

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