Vesicle-Mediated Gene Transfer in Acinetobacter baumannii

Fulsundar, Shweta; Domingues, Sara; Nielsen, Kaare Magne

doi:10.1007/978-1-4939-9118-1_9

Cited by 6 publications

(11 citation statements)

References 24 publications

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“…Accordingly, the plasmid sequence analysis failed to identify any plasmid transfer operons. Other mechanisms, including natural transformation, transduction, and/or outer membrane vesicle (OMV)mediated transfer, may contribute to the HGT of this plasmid (17)(18)(19), which deserves further study. Nevertheless, our study clearly demonstrated that these antibioticresistant plasmids or strains are spreading in waterfowls and their neighboring environments in China.…”

Section: Discussionmentioning

confidence: 99%

Co-occurrence of Plasmid-Mediated Tigecycline and Carbapenem Resistance in Acinetobacter spp. from Waterfowls and Their Neighboring Environment

Cui

Chen

Liu

et al. 2020

Antimicrob Agents Chemother

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Tigecycline serves as one of the antibiotics of last resort to treat multidrug-resistant (including carbapenem-resistant) pathogens. However, the recently emerged plasmid-mediated tigecycline resistance mechanism, Tet(X), challenges the clinical efficacy of this class of antibiotics. In this study, we detected 180 tet(X)-harboring Acinetobacter isolates (8.9%, n = 180) from 2,018 samples collected from avian farms and adjacent environments in China. Eighteen tet(X)-harboring isolates (10.0%) were found to cocarry the carbapenemase gene blaNDM-1, mostly from waterfowl samples (94.4%, 17/18). Interestingly, among six Acinetobacter strains, tet(X) and blaNDM-1 were found to colocalize on the same plasmids. Moreover, whole-genome sequencing (WGS) revealed a novel orthologue of tet(X) in the six isolates coharboring tet(X) and blaNDM-1. Inverse PCR suggested that the two tet(X) genes form a single transposable unit and may be cotransferred. Sequence comparison between six tet(X)- and blaNDM-1-coharboring plasmids showed that they shared a highly homologous plasmid backbone even though they were isolated from different Acinetobacter species (three from Acinetobacter indicus, two from Acinetobacter schindleri, and one from Acinetobacter lwoffii) from various sources and from different geological regions, suggesting the horizontal genetic transfer of a common tet(X)- and blaNDM-1-coharboring plasmid among Acinetobacter species in China. Emergence and spread of such plasmids and strains are of great clinical concern, and measures must be implemented to avoid their dissemination.

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Section: Discussionmentioning

confidence: 99%

Co-occurrence of Plasmid-Mediated Tigecycline and Carbapenem Resistance in Acinetobacter spp. from Waterfowls and Their Neighboring Environment

Cui

Chen

Liu

et al. 2020

Antimicrob Agents Chemother

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“…Transformation experiment was performed according to a method described previously by Fulsundar et al [12] (Fig 1)…”

Section: Omv Mediated Gene Transfermentioning

confidence: 99%

“…The lumen of the vesicles contains periplasmic proteins, cytosolic components, and nucleic acids [11]. OMVs also carry DNA and RNA on their surface, which can be removed by OMV treatment with DNase and RNase, whereas luminal DNA and RNA are nor affected by the treatment [12].…”

Section: Ntroductionmentioning

confidence: 99%

Outer Membrane Vesicle Mediated Multidrug Resistance Gene Transfer in Avibacterium Paragallinarum

Chen

Zhi

et al. 2022

Preprint

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Infectious coryza is an acute upper respiratory tract infectious disease caused by Avibacterium paragallinarum, which can cause growth retardation and egg production decline of bred chickens, and bring great economic losses to poultry industry. A. Paragallinarum is a Gram-negative bacterium andcan release outer membrane vesicles (OMVs).In this study, a comparative genomic analysis of A. Paragallinarumisolate P4chr1 and its OMVs were carried out, and the ability to transfer antibiotic resistance genes via the OMVswas studied. The sequencing and data analysis showed that genome size of A. paragallinarum P4chr1 is about 2.77 Mb and it has a 25 kb tolerance island covering 6 types of antibiotics and 11 resistance genes. The genome size of its OMVs is about 2.69 Mb, covering 97% genome length and almost all gene sequences of P4chr1. When purified and DNase treated A. paragallinarum P4chr1 OMVs were co-cultured with antibiotic sensitive A. paragallinarum Modesto strain on an antibiotic containing plate, the colonies grown on the plate were detected corresponding antibiotic resistance gene (ARG).However, antimicrobial susceptibility test exposed that drug resistance genes delivered by OMVs were not persistent, they only existed temporarily on the antibiotic plates. The antibiotic resistance and ARGs disappeared at second bacterial passage. Overall, this study is the first report to compare genomic characteristics of OMVs with its parent A. paragallinarum strain, and to study A. paragallinarum ARG transfer via OMVs. This work has provided useful data for further study on the issue of non-plasmid ARG transfer mediated by A. paragallinarum OMVs.

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“…OMVs carry DNA and RNA on their surface or in the vesicular lumen. A clear difference can be observed by OMV treatment with DNase and RNase: luminal DNA and RNA are preserved even after the enzymatic treatment [ 40 , 42 ]. Several studies showed the presence of DNA in the OMV lumen of Escherichia coli [ 43 ], Neisseria gonorrhoeae [ 44 ], P. aeruginosa [ 45 ], Acinetobacter baumannii [ 46 ], etc.…”

Section: Omvs: An Overview From Structure To Functionmentioning

confidence: 99%

Gene Transfer Potential of Outer Membrane Vesicles of Gram-Negative Bacteria

Dell’Annunziata

Folliero

Giugliano

et al. 2021

IJMS

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The increasing spread of multidrug-resistant pathogenic bacteria is one of the major threats to public health worldwide. Bacteria can acquire antibiotic resistance and virulence genes through horizontal gene transfer (HGT). A novel horizontal gene transfer mechanism mediated by outer membrane vesicles (OMVs) has been recently identified. OMVs are rounded nanostructures released during their growth by Gram-negative bacteria. Biologically active toxins and virulence factors are often entrapped within these vesicles that behave as molecular carriers. Recently, OMVs have been reported to contain DNA molecules, but little is known about the vesicle packaging, release, and transfer mechanisms. The present review highlights the role of OMVs in HGT processes in Gram-negative bacteria.

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Vesicle-Mediated Gene Transfer in Acinetobacter baumannii

Cited by 6 publications

References 24 publications

Co-occurrence of Plasmid-Mediated Tigecycline and Carbapenem Resistance in Acinetobacter spp. from Waterfowls and Their Neighboring Environment

Co-occurrence of Plasmid-Mediated Tigecycline and Carbapenem Resistance in Acinetobacter spp. from Waterfowls and Their Neighboring Environment

Outer Membrane Vesicle Mediated Multidrug Resistance Gene Transfer in Avibacterium Paragallinarum

Gene Transfer Potential of Outer Membrane Vesicles of Gram-Negative Bacteria

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