Vesicle formation mechanisms: an overview

Has, Chandra; Pan, Sharadwata

doi:10.1080/08982104.2020.1730401

Cited by 47 publications

(27 citation statements)

References 202 publications

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“…Moreover, the measure of ζ potential confirmed the presence of a net negative surface charge for those liposomal dispersions carrying AS (Table 2), evidencing that the extrusion process slightly affected the net charge of anionic liposomes in agreement with the literature data [40]. Furthermore, each sample displays a ζ potential greater than 30 mV in absolute value, thus, the dispersion is particularly stable in view of the electrostatic repulsion developed between the charges of the same sign avoiding the agglomeration of the liposomal vesicles [41]. The comparison between the values of the ζ potential (Table 2), highlights the different influence of the AS addition on the liposomes.…”

Section: Liposome Production and Characterizationsupporting

confidence: 88%

Manganese in Diagnostics: A Preformulatory Study

et al. 2022

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This investigation aims to find lipid-based nanosystems to be used as tools to deliver manganese for diagnostic purposes in multimodal imaging techniques. In particular, the study describes the production and characterization of aqueous dispersions of anionic liposomes as delivery systems for two model manganese-based compounds, namely manganese chloride and manganese acetylacetonate. Negatively charged liposomes were obtained using four different anionic surfactants, namely sodium docusate (SD), N-lauroylsarcosine (NLS), Protelan AG8 (PAG) and sodium lauroyl lactylate (SLL). Liposomes were produced by the direct hydration method followed by extrusion and characterized in terms of size, polydispersity, surface charge and stability over time. After extrusion, liposomes are homogeneous and monodispersed with an average diameter not exceeding 200 nm and a negative surface charge as confirmed by ζ potential measurement. Moreover, as indicated by atomic absorption spectroscopy analyses, the loading of manganese-based compounds was almost quantitative. Liposomes containing NLS or SLL were the most stable over time and the presence of manganese-based compounds did not affect their size distribution. Liposomes containing PAG and SD were instable and therefore discarded. The in vitro cytotoxicity of the selected anionic liposomes was evaluated by MTT assay on human keratinocyte. The obtained results highlighted that the toxicity of the formulations is dose dependent.

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Section: Liposome Production and Characterizationsupporting

confidence: 88%

Manganese in Diagnostics: A Preformulatory Study

et al. 2022

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“…The formation of vesicles by the generally applied hydration method from stacked DOPG bilayers, as proposed in Figure 14, was also investigated by the combined SHG, TPF and Rayleigh scattering approaches. This formation process in Figure 14 was generally accepted in previous reports [72][73][74]. The combined multiple spectral methods were demonstrated to be capable of revealing the detailed structural evolution of the lipids during this process [47].…”

Section: Investigating the Vesicle Formation Process And Dynamics Of ...supporting

confidence: 78%

Combined Second Harmonic Generation and Fluorescence Analyses of the Structures and Dynamics of Molecules on Lipids Using Dual-Probes: A Review

Hou

et al. 2022

Molecules

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Revealing the structures and dynamic behaviors of molecules on lipids is crucial for understanding the mechanism behind the biophysical processes, such as the preparation and application of drug delivery vesicles. Second harmonic generation (SHG) has been developed as a powerful tool to investigate the molecules on various lipid membranes, benefiting from its natural property of interface selectivity, which comes from the principle of even order nonlinear optics. Fluorescence emission, which is in principle not interface selective but varies with the chemical environment where the chromophores locate, can reveal the dynamics of molecules on lipids. In this contribution, we review some examples, which are mainly from our recent works focusing on the application of combined spectroscopic methods, i.e., SHG and two-photon fluorescence (TPF), in studying the dynamic behaviors of several dyes or drugs on lipids and surfactants. This review demonstrates that molecules with both SHG and TPF efficiencies may be used as intrinsic dual-probes in plotting a clear physical picture of their own behaviors, as well as the dynamics of other molecules, on lipid membranes.

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“…The large-scale Current Good Manufacturing Practice (cGMP) manufacturing processes and corresponding CPPs are mapped in Figure 2, while relevant methodology and details behind individual CQA-CPP relationships are displayed in Tables S4-S11 in the supplemental information online. Despite the scarcity of large-scale and RNA-specific data, numerous in-process risks can still be identified due to the repurposing of multiple unit operations and our increased mechanistic understanding of RNA and LNPs as biophysical systems [56][57][58]. Although in-process stability is not yet precisely characterized, there are well-defined degradation pathways described in the literature for nucleic acid-and lipidbased delivery systems.…”

Section: Knowledge Assessment Of Product-process Interactionsmentioning

confidence: 99%