Very small size proteoliposomes derived from Neisseria meningitidis: An effective adjuvant for generation of CTL responses to peptide and protein antigens

Induction of leukocyte infiltration at metastatic site mediates the protective effect of NGcGM3-based vaccine

“…32 In the spontaneous tumor model that we used, Katz et al 33 reported a complete protection against spontaneous metastases after combining human recombinant IL-6 and irradiated 3LL-D122 tumor cells. Our results are in accordance also with the ones published by Mesa et al, 34 who used the acetylated variant of GM3 ganglioside integrated into VSSP evidencing a potent activation and cytokine secretion of cDC, suggesting that the bacterial component of the vaccine is relevant for APC activation and function.…”

Inhibition of Tumor-Induced Myeloid-Derived Suppressor Cell Function by a Nanoparticulated Adjuvant

“…Previous in vivo experiments using similar dosage and schedule of VSSP suggested that VSSP-MDSCs could display a reduced functional activity because VSSP potentiated Ag-specific CTL responses (27). These results suggest that MDSCs recruited during acute exposure to VSSP might exert a less suppressive function than that of counterparts induced during a chronic inflammation and tumor growth.…”

“…Transgenic mice expressing a TCR specific for aa 512-520 from influenza hemagglutinin (HA) presented by H-2K d (CL4 mice) were a gift from L. Sherman (The Scripps Research Institute, La Jolla, CA). Thy1.1 + congenic mice on BALB/c background and pmel-1 mice expressing transgenic TCRs that recognize gp100 [25][26][27][28][29][30][31][32][33] peptide in an H-2D b -restricted manner were provided by N. Restifo (National Cancer Institute, Bethesda, MD). OT-I TCR transgenic mice and CD45.1 + congenic mice on C57BL/6 genetic background were purchased from The Jackson Laboratory (Bar Harbor, ME).…”

“…This product is currently under investigation as part of the formulation of several cancer vaccine candidates at different development stages (4,(24)(25)(26). VSSP promotes dendritic cell (DC) maturation, Ag crosspresentation to CD8 + T cells, Th1 polarization, and enhances CTL response (27,28). However, the capacity of VSSP to influence regulatory cells has not previously been addressed.…”

Selection of Adjuvants for Enhanced Vaccine Potency

“…Proteoliposomes in different sizes contain bacterial membrane components (e.g. the outer membrane of N. meningitidis B), including LPS, phospholipids, and traces of bacterial DNA to improve immunogenicity [190,191]. Cochleate structures (AFCo1) are made through interaction of divalent cations with anionic lipids in proteoliposomes.…”