Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors

Cutarelli, Alessandro; Ghio, Simone; Zasso, Jacopo; Speccher, Alessandra; Scarduelli, Giorgina; Roccuzzo, Michela; Crivellari, M.; Pugno, Nicola; Casarosa, Simona; Boscardin, M.; Conti, Luciano

doi:10.3390/cells9010088

Cited by 25 publications

(26 citation statements)

References 57 publications

(68 reference statements)

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“…This Special Issue includes articles highlighting the effective use of induced pluripotent stem cells (iPSCs) to develop cardiac microtissue [ 1 ], neurospheres [ 2 ], and cortical progenitors [ 3 ]. In addition, this Special Issue contains work on scaffolds with mesenchymal stem cells (MSCs) [ 4 ] and embryonic stem cells (ESCs) [ 5 ].…”

mentioning

confidence: 99%

“…The work by Cutarelli et al employed iPSC-derived cortical progenitors and scaffolds to mimic the radially oriented cortical radial glia fibers that play an important role in the development of the cerebral cortex [ 3 ]. The authors used silicon vertical micropillar arrays to promote the expansion and preservation of stemness of the cortical progenitors.…”

mentioning

confidence: 99%

“…The authors used silicon vertical micropillar arrays to promote the expansion and preservation of stemness of the cortical progenitors. Furthermore, the described model of iPSC-derived cortical progenitors and silicon micropillars could be used in cortical tissue engineering [ 3 ].…”

mentioning

confidence: 99%

“…This Special Issue includes articles highlighting recent discoveries in 3D stem cell cultures using MSCs [ 4 ], ESCs [ 5 , 8 ], iPSCs [ 1 , 2 , 3 ], and intestinal stem cells [ 6 , 7 ]. This Special Issue also includes a review article by Ribeiro-Filho et al that discusses the 2D and 3D culture of hematopoietic stem cells (HSCs) as a relevant model to study both normal and abnormal hematopoiesis [ 9 ].…”

mentioning

confidence: 99%

“…This Special Issue also includes a review article by Ribeiro-Filho et al that discusses the 2D and 3D culture of hematopoietic stem cells (HSCs) as a relevant model to study both normal and abnormal hematopoiesis [ 9 ]. Furthermore, this Special Issue includes articles that utilize various scaffolds together with stem cells from various sources to study 3D cultures [ 3 , 4 , 5 ].…”

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confidence: 99%

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3D Stem Cell Culture

Ylöstalo

2020

Cells

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Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seeking optimal stem cell preparations for basic research and clinical applications, and these might be attainable through 3D culture of stem cells. The 3D cultures recapitulate the in vivo cell-to-cell and cell-to-matrix interactions more effectively, and the cells in 3D cultures exhibit many unique and desirable characteristics. The culture of stem cells in 3D may employ various matrices or scaffolds, in addition to the cells, to support the complex structures. The goal of this Special Issue is to bring together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cells and culture techniques, and also highlight stem cell preparations for possible novel therapeutic applications.

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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3D Stem Cell Culture

Ylöstalo

2020

Cells

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Emerging Brain‐Pathophysiology‐Mimetic Platforms for Studying Neurodegenerative Diseases: Brain Organoids and Brains‐on‐a‐Chip

Bang

Lee

Choi

et al. 2021

Adv Healthcare Materials

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Neurodegenerative diseases are a group of disorders characterized by progressive degeneration of the structural and functional integrity of the central and peripheral nervous systems. Millions of people suffer from degenerative brain diseases worldwide, and the mortality continues to increase every year, causing a growing demand for knowledge of the underlying mechanisms and development of therapeutic targets. Conventional 2D‐based cell culture platforms and animal models cannot fully recapitulate the pathophysiology, and this has limited the capability for estimating drug efficacy. Recently, engineered platforms, including brain organoids and brain‐on‐a‐chip, have emerged. They mimic the physiology of brain tissue and reflect the fundamental pathophysiological signatures of neurodegenerative diseases, such as the accumulation of neurotoxic proteins, structural abnormalities, and functional loss. In this paper, recent advances in brain‐mimetic platforms and their potential for modeling features of neurodegenerative diseases in vitro are reviewed. The development of a physiologically relevant model should help overcome unresolved neurodegenerative diseases.

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Neuromechanobiology: An Expanding Field Driven by the Force of Greater Focus

Motz

Kabat

Saxena

et al. 2021

Adv Healthcare Materials

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The brain processes information by transmitting signals through highly connected and dynamic networks of neurons. Neurons use specific cellular structures, including axons, dendrites and synapses, and specific molecules, including cell adhesion molecules, ion channels and chemical receptors to form, maintain and communicate among cells in the networks. These cellular and molecular processes take place in environments rich of mechanical cues, thus offering ample opportunities for mechanical regulation of neural development and function. Recent studies have suggested the importance of mechanical cues and their potential regulatory roles in the development and maintenance of these neuronal structures. Also suggested are the importance of mechanical cues and their potential regulatory roles in the interaction and function of molecules mediating the interneuronal communications. In this review, the current understanding is integrated and promising future directions of neuromechanobiology are suggested at the cellular and molecular levels. Several neuronal processes where mechanics likely plays a role are examined and how forces affect ligand binding, conformational change, and signal induction of molecules key to these neuronal processes are indicated, especially at the synapse. The disease relevance of neuromechanobiology as well as therapies and engineering solutions to neurological disorders stemmed from this emergent field of study are also discussed.

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Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors

Cited by 25 publications

References 57 publications

3D Stem Cell Culture

3D Stem Cell Culture

Emerging Brain‐Pathophysiology‐Mimetic Platforms for Studying Neurodegenerative Diseases: Brain Organoids and Brains‐on‐a‐Chip

Neuromechanobiology: An Expanding Field Driven by the Force of Greater Focus

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