Vertical ridge augmentation using guided bone regeneration procedure and dental pulp derived mesenchymal stem cells with simultaneous dental implant placement: A histologic study in a sheep model

Çolpak, Halis Ali; Gönen, Zeynep Burçin; Özdamar, Saim; Alkan, Alper; Kütük, Nükhet

doi:10.1016/j.jormas.2018.12.011

Cited by 19 publications

(21 citation statements)

References 29 publications

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“…The histological evaluation was performed for a total of 28 of the selected papers (93.3%) and histomorphometry was conducted in 25 of the studies' experiments [45][46][47][48][49][50][51][52][53][54][55][56][57][58]60,[62][63][64][65][66][67][68][69][70][71] (83.3%). For a total of 18 studies (60%) micro-CT evaluation was performed [44][45][46][47][48][49][50][54][55][56][57][58][59]63,65,68,70,71] and immunohistochemistry was performed for 26 articles (86.7%) [45][46][47][48][49][50]…”

Section: General Parametersmentioning

confidence: 99%

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Synthetic Scaffold/Dental Pulp Stem Cell (DPSC) Tissue Engineering Constructs for Bone Defect Treatment: An Animal Studies Literature Review

Lorusso

Inchingolo

Dipalma

et al. 2020

IJMS

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Background: Recently a greater interest in tissue engineering for the treatment of large bone defect has been reported. The aim of the present systematic review and meta-analysis was to investigate the effectiveness of dental pulp stem cells and synthetic block complexes for bone defect treatment in preclinical in vivo articles. Methods: The electronic database and manual search was conducted on Pubmed, Scopus, and EMBASE. The papers identified were submitted for risk-of-bias assessment and classified according to new bone formation, bone graft characteristics, dental pulp stem cells (DPSCs) culture passages and amount of experimental data. The meta-analysis assessment was conducted to assess new bone formation in test sites with DPSCs/synthetic blocks vs. synthetic block alone. Results: The database search identified a total of 348 papers. After the initial screening, 30 studies were included, according to the different animal models: 19 papers on rats, 3 articles on rabbits, 2 manuscripts on sheep and 4 papers on swine. The meta-analysis evaluation showed a significantly increase in new bone formation in favor of DPSCs/synthetic scaffold complexes, if compared to the control at 4 weeks (Mean Diff: 17.09%, 95% CI: 15.16–18.91%, p < 0.01) and at 8 weeks (Mean Diff: 14.86%, 95% CI: 1.82–27.91%, p < 0.01) in rats calvaria bone defects. Conclusion: The synthetic scaffolds in association of DPSCs used for the treatment of bone defects showed encouraging results of early new bone formation in preclinical animal studies and could represent a useful resource for regenerative bone augmentation procedures

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Section: General Parametersmentioning

confidence: 99%

“…A total of three studies were performed on rabbit models and the DPSC culture passage ranged from three to seven [65][66][67]. One article was conducted on femur diaphysis [66], one article on [67], and one study on calvaria/critical size defect [65].…”

Section: Rabbit Study Modelmentioning

confidence: 99%

Synthetic Scaffold/Dental Pulp Stem Cell (DPSC) Tissue Engineering Constructs for Bone Defect Treatment: An Animal Studies Literature Review

Lorusso

Inchingolo

Dipalma

et al. 2020

IJMS

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“…Regarding dental regeneration, both sheep and goat are reasonable candidates as animal models, although not widely used for this field. Nonetheless, various studies have used bone grafting techniques associated with dental stem cell therapy, such as dental pulp stem cells [139,145,146].…”

Section: Osteoarticular Systemmentioning

confidence: 99%

Small Ruminants and Its Use in Regenerative Medicine: Recent Works and Future Perspectives

Alvites

Branquinho

Sousa

et al. 2021

Biology

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Medical and translational scientific research requires the use of animal models as an initial approach to the study of new therapies and treatments, but when the objective is an exploration of translational potentialities, classical models fail to adequately mimic problems in humans. Among the larger animal models that have been explored more intensely in recent decades, small ruminants, namely sheep and goats, have emerged as excellent options. The main advantages associated to the use of these animals in research works are related to their anatomy and dimensions, larger than conventional laboratory animals, but very similar to those of humans in most physiological systems, in addition to their low maintenance and feeding costs, tendency to be docile, long life expectancies and few ethical complications raised in society. The most obvious disadvantages are the significant differences in some systems such as the gastrointestinal, and the reduced amount of data that limits the comparison between works and the validation of the characterization essays. Despite everything, recently these species have been increasingly used as animal models for diseases in different systems, and the results obtained open doors for their more frequent and advantageous use in the future. The purpose of this review is to summarize the general principles related to the use of small ruminants as animal models, with a focus on regenerative medicine, to group the most relevant works and results published recently and to highlight the potentials for the near future in medical research.

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“…Studies focus on MSCs immunomodulatory effects to induce regeneration of dental and periodontal tissues, and differentiation potential of MSCs to improve implant strength and bone tissue repair in the alveolar defects. In addition to usual sources of stem cells such as bone marrow and adipose tissue, cells derived from local tissues such as dental pulp stem cells (DPSC) (200)(201)(202) or periodontal ligament stem cells (PDLSC) (203,204) are studied as a therapeutic option in orodental diseases. In experimental dog models, autologous BMMSCs or xenogeneic periodontal ligament MSCs have proven beneficial in periodontal ligament reconstruction, when combined with the growth factors (205), fibrin glue, PRP (206), ephrinB2-a membrane protein regulating bone homeostasis (204) or with a construct of porous biphasic calcium phosphate (203).…”

Section: Orodental Diseasesmentioning

confidence: 99%

Stem Cells in Veterinary Medicine—Current State and Treatment Options

et al. 2020

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Regenerative medicine is a branch of medicine that develops methods to grow, repair, or replace damaged or diseased cells, organs or tissues. It has gained significant momentum in recent years. Stem cells are undifferentiated cells with the capability to self-renew and differentiate into tissue cells with specialized functions. Stem cell therapies are therefore used to overcome the body's inability to regenerate damaged tissues and metabolic processes after acute or chronic insult. The concept of stem cell therapy was first introduced in 1991 by Caplan, who proposed that massive differentiation of cells into the desired tissue could be achieved by isolation, cultivation, and expansion of stem cells in in vitro conditions. Among different stem cell types, mesenchymal stem cells (MSC) currently seem to be the most suitable for therapeutic purposes, based on their simple isolation and culturing techniques, and lack of ethical issues regarding their usage. Because of their remarkable immunomodulatory abilities, MSCs are increasingly gaining recognition in veterinary medicine. Developments are primarily driven by the limitations of current treatment options for various medical problems in different animal species. MSCs represent a possible therapeutic option for many animal diseases, such as orthopedic, orodental and digestive tract diseases, liver, renal, cardiac, respiratory, neuromuscular, dermal, olfactory, and reproductive system diseases. Although we are progressively gaining an understanding of MSC behavior and their mechanisms of action, some of the issues considering their use for therapy are yet to be resolved. The aim of this review is first to summarize the current knowledge and stress out major issues in stem cell based therapies in veterinary medicine and, secondly, to present results of clinical usage of stem cells in veterinary patients.

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Vertical ridge augmentation using guided bone regeneration procedure and dental pulp derived mesenchymal stem cells with simultaneous dental implant placement: A histologic study in a sheep model

Cited by 19 publications

References 29 publications

Synthetic Scaffold/Dental Pulp Stem Cell (DPSC) Tissue Engineering Constructs for Bone Defect Treatment: An Animal Studies Literature Review

Synthetic Scaffold/Dental Pulp Stem Cell (DPSC) Tissue Engineering Constructs for Bone Defect Treatment: An Animal Studies Literature Review

Small Ruminants and Its Use in Regenerative Medicine: Recent Works and Future Perspectives

Stem Cells in Veterinary Medicine—Current State and Treatment Options

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