Vertical Mobility of Soil Contaminants: Preliminary Results of a Survey on the Herbicide Atrazine

Muccio, Alfonso Di; Chirico, Mattea; Dommarco, Roberto; Funari, Enzo; Musmeci, Loredana; Santilio, Angela; Vergori, Fabio; Zapponi, G.; Giuliano, G.; Sparacino, A. C.

doi:10.1080/03067319008026928

Cited by 10 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This biomass represents a diversity of species that is a dynamic population able to degrade a wide variety of chemically complex compounds, including herbicides [5-71. Atrazine biodegradation was reviewed by Kaufman and Kearney [8] in 1970. Since then, studies have focused on water contamination and on dissipation, persistence, and leaching from soil [9,10]. Little information is available concerning the microbial catabolism of atrazine [ll-131, and its potential effects on natural ecosystems remain controversial.…”

Section: Introductionmentioning

confidence: 99%

Degradation of atrazine in laboratory microcosms: Isolation and identification of the biodegrading bacteria

Mirgain

Green

Monteil

1993

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Several natural ecosystems, including soil and water microcosms, have been explored for their potential to biodegrade atrazine (2‐chloro‐4‐[ethylamino]‐6‐[isopropylamino]‐1,3,5‐triazine). Subculturing soil or water samples with several applications of the herbicide leads to an enhancement of atrazine catabolism correlating with a decrease in the number of bacterial species. A combination of enrichment culture techniques, the imposition of carbon limitation, and plating on selective atrazine‐agar plates have permitted the isolation of several bacteria able to biodegrade atrazine from different polluted or unpolluted ecosystems. One of the selected bacteria, originating from an agricultural soil, has been identified as Acinetobacter calcoaceticus, which alone in pure culture can degrade atrazine. From another agricultural soil Pseudomonas alcaligenes associated with an Agrobactenum sp. was selected. A garden soil treated only once with the herbicide was found to contain an association of five different bacteria that together degrade atrazine. Finally, a Pseudomonas putida/Xanthomonas maltophilia pair has been isolated from an aquatic ecosystem. In consortium, in coculture, or in pure culture with well‐defined experimental parameters, the induced bacteria degrade added atrazine in 18 h.

show abstract

Section: Introductionmentioning

confidence: 99%

Degradation of atrazine in laboratory microcosms: Isolation and identification of the biodegrading bacteria

Mirgain

Green

Monteil

1993

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

Development of an Enzyme Immunoassay for the Analysis of the Atrazine Metabolite Hydroxyatrazine Entwicklung eines Enzymimmunoassays zur Analyse des Atrazin‐Metaboliten Hydroxyatrazin

Wittmann¹,

Hock²

1994

Acta hydrochim. hydrobiol.

View full text Add to dashboard Cite

Summary: A highly sensitive and specific enzyme immunoassay (EIA) is described for the detection of the atrazine metabolite hydroxyatrazine. Polyclonal antibodies were raised in rabbits by immunization with a hapten-bovine serum albumin (BSA) conjugate containing 8 hapten residues per molecule of BSA. An EIA with a horseradish peroxidase (HRP) hapten tracer was optimized in microtitre plates. A concentration of 50% B/Bo was found at 0.10 pg/L for hydroxyatrazine. A limit of determination for hydroxyatrazine was reached at approximately 0.01 pg/L, i. e. well below the maximum concentration permitted by the EU guidelines for drinking water and the drinking water ordinance of the FRG. The assay did not require concentration or clean-up steps for drinking water or ground water samples. Validation experiments confirmed a good accuracy and precision. Hydroyatrazine is reported to be the main atrazine metabolite found in soil samples. As organic solvents are usually employed for soil extraction, the influence of methanol as representative organic solvent on the assay was examined. Up to a concentration of 5% (vh) methanol, the organic solvent did not affect the assay. Schlagworter

show abstract

Pollution potential of selected pesticides in soils

Raju¹,

Millette²,

Khan³

1993

Chemosphere

View full text Add to dashboard Cite

Vertical Mobility of Soil Contaminants: Preliminary Results of a Survey on the Herbicide Atrazine

Cited by 10 publications

References 10 publications

Degradation of atrazine in laboratory microcosms: Isolation and identification of the biodegrading bacteria

Degradation of atrazine in laboratory microcosms: Isolation and identification of the biodegrading bacteria

Development of an Enzyme Immunoassay for the Analysis of the Atrazine Metabolite Hydroxyatrazine Entwicklung eines Enzymimmunoassays zur Analyse des Atrazin‐Metaboliten Hydroxyatrazin

Pollution potential of selected pesticides in soils

Contact Info

Product

Resources

About