Vertical Hydrodynamic Focusing and Continuous Acoustofluidic Separation of Particles via Upward Migration

Ahmed, Husnain; Destgeer, Ghulam; Park, Jinsoo; Jung, Jin Ho; Sung, Hyung Jin

doi:10.1002/advs.201700285

Cited by 40 publications

(24 citation statements)

References 52 publications

(55 reference statements)

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“…The small dimensions characteristic of microfluidic devices has enabled selective manipulation of similarly small objects such as cells and microparticles (Lee et al 2017;Di Carlo 2009;Tayebi et al 2020), including for tissue engineering (Choi et al 2007; Andersson and van den Berg 2004; Khademhosseini et al 2006;Novak et al 2020;Bhatia and Ingber 2014), cell-cell interaction and signalling studies (Regehr et al 2009;Faley et al 2008;Zervantonakis et al 2011), sample concentration and sorting (Ding et al 2012;Gascoyne and Vykoukal 2002;Ahmed et al 2018). This accurate and versatile manipulation of biological matter is essential for many lab-on-a-chip platforms, especially those designed for diagnostic purposes.…”

Section: Introductionmentioning

confidence: 99%

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

Devendran

Collins

Neild

2022

Microfluid Nanofluid

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Surface acoustic wave (SAW) micromanipulation offers modularity, easy integration into microfluidic devices and a high degree of flexibility. A major challenge for acoustic manipulation, however, is the existence of a lower limit on the minimum particle size that can be manipulated. As particle size reduces, the drag force resulting from acoustic streaming dominates over acoustic radiation forces; reducing this threshold is key to manipulating smaller specimens. To address this, we investigate a novel excitation configuration based on diffractive-acoustic SAW (DASAW) actuation and demonstrate a reduction in the critical minimum particle size which can be manipulated. DASAW exploits the inherent diffractive effects arising from a limited transducer area in a microchannel, requiring only a travelling SAW (TSAW) to generate time-averaged pressure gradients. We show that these acoustic fields focus particles at the channel walls, and further compare this excitation mode with more typical standing SAW (SSAW) actuation. Compared to SSAW, DASAW reduces acoustic streaming effects whilst generating a comparable pressure field. The result of these factors is a critical particle size with DASAW (1 $$\upmu$$ μ m) that is significantly smaller than that for SSAW actuation (1.85 $$\upmu$$ μ m), for polystyrene particles and a given $$\lambda _{\text {SAW}}$$ λ SAW = 200 $$\upmu$$ μ m. We further find that streaming magnitude can be tuned in a DASAW system by changing the channel height, noting optimum channel heights for particle collection as a function of the fluid wavelength at which streaming velocities are minimised in both DASAW and SSAW devices.

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Section: Introductionmentioning

confidence: 99%

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

Devendran

Collins

Neild

2022

Microfluid Nanofluid

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“…Nano/micromotors are nano/microscale devices that can convert environmental energy, e.g., chemical, magnetic, light, thermal, electric, and acoustic energies into mechanical energy. Wondrous biomimetic behaviors, including cargo transportation, chemotaxis, phototaxis, swarming, and rheotaxis, have been disclosed in the past decade.…”

Section: Introductionmentioning

confidence: 99%

Light‐Ultrasound Driven Collective “Firework” Behavior of Nanomotors

et al. 2018

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It is of great interest and big challenge to control the collective behaviors of nanomotors to mimic the aggregation/separation behavior of biological systems. Here, a light‐acoustic combined method is proposed to control the aggregation/separation of artificial nanomotors. It is shown that nanomotors aggregate at the pressure node in acoustic field and afterward present a collective “firework” separation behavior induced by light irradiation. The collective behavior is found to be applicable for metallic materials and polymers even different light wavelengths are used. Physical insights on the collective firework behavior resulting from the change of acoustic streaming caused by optical force are provided. It is found that diffusion velocity and diffusion region of cluster can be controlled by adjusting light intensity and acoustic excitation voltage, and irradiation direction, respectively. This harmless, controllable, and widely applicable method provides new possibilities for groups of nanomachines, drug release, and cargo transport in nanomedicine and nanosensors.

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“…The benefits of operating at a reduced size scale have enabled highly efficient techniques for selective patterning and manipulating cells . Such approaches have been used for a wide range of tasks, including single cell analysis, tissue engineering, studying cell–cell interaction and signaling, sorting, and drug screening . As the applications of this technology are focused within the clinical and life sciences, a thorough understanding of the associated biological impact imposed by these manipulation techniques is necessary.…”

Section: Introductionmentioning

confidence: 99%

Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems

et al. 2019

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As the applications of this technology are focused within the clinical and life sciences, a thorough understanding of the associated biological impact imposed by these manipulation techniques is necessary.The desire to manipulate suspended matter within microfluidic systems has inspired a range of techniques both passive [5,19,20] and active. [21,22] Passive approaches rely heavily on the geometry of the microfluidic channels and inertial forces. Flow profiles are altered by the introduction of sudden expansions and contractions, weirs and pillars to impede and divert the flow into desired streamlines. This reliance on the resultant flow profile restricts the flexibility of the chip, being single task specific. In contrast, active methods are significantly more robust, capable of on-demand actuation and offer the ability to change functionality ad-hoc, leading to an increased selectivity. To this end, a range of active methods have been developed using magnetic, [23,24] optical, [25,26] electrical [16,27] and acoustic [28][29][30] excitation.Acoustofluidics is the use of acoustic forces to manipulate suspended matter within microfluidics, [31,32] and has the advantage of uniquely combining ease of on-chip integration and simple, yet dextrous establishment of force fields in a noncontact manner. [29,33,34] As a direct result, it has been extensively used to capture, [33,35] pattern, [36][37][38] and sort particles, [15,34,39] cell sonoporation, [40] synthesize nanomaterials, [41] as well as to mix fluids. [42,43] Although acoustofluidics has been widely accepted as a biocompatible technique, substantiated with cell viability studies; [44][45][46][47][48] there have been no extensive viability studies at elevated frequencies (30-600 MHz). Indeed, typically studies are corroborated with a single viability method, most commonly live/dead staining, [6,[49][50][51] or trypan blue exclusion [52,53] and in some instances proliferation studies (MTT). [36] In contrast to these singular approaches, in passive microfluidic systems in which cells are predominantly subjected to shear forces arising from the flow field, a wide range of multifaceted cell viability studies [54] have been conducted showing, for example, sheardependent regulation of the von Willebrand factor of human umbilical vein endothelial cells [55] and the potential for circulating tumor cell apoptosis at high shear levels. [56] This lack of biological knowledge may result in potential unrecognized adverse effects (i.e., "false positives"), or Acoustic fields are capable of manipulating biological samples contained within the enclosed and highly controlled environment of a microfluidic chip in a versatile manner. The use of acoustic streaming to alter fluid flows and radiation forces to control cell locations has important clinical and life science applications. While there have been significant advances in the fundamental implementation of these acoustic mechanisms, there is a considerable lack of understanding of the associated biological effects on cells. ...

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Vertical Hydrodynamic Focusing and Continuous Acoustofluidic Separation of Particles via Upward Migration

Cited by 40 publications

References 52 publications

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

Light‐Ultrasound Driven Collective “Firework” Behavior of Nanomotors

Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems

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