Vertical distribution of Fe and Fe(III)-reducing bacteria in the sediments of Lake Donghu, China

Tian, Cuicui; Wang, Chunbo; Tian, Yingying; Wu, Xingqiang; Xiao, Baihua

doi:10.1139/cjm-2015-0129

Cited by 12 publications

(6 citation statements)

References 59 publications

(77 reference statements)

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“…6 However, so far, information about their roles in fresh lake sediments has been very limited. 25 A recent work revealed that EEB were largely involved in the biogeochemical Fe cycling in hypersaline sediments of a salt lake, 12 but their abundance in hypersaline sediments was much lower than that in fresh lake sediments obtained in this study. Such a difference implies that EEB might be involved in the degradation of organic matter and possibly biogeochemical cycling of other elements in fresh environments.…”

Section: Environmental Science and Technology Letterscontrasting

confidence: 58%

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Rapid Detection and Enumeration of Exoelectrogenic Bacteria in Lake Sediments and a Wastewater Treatment Plant Using a Coupled WO₃ Nanoclusters and Most Probable Number Method

Yang

Cheng

Zhang

et al. 2016

Environ. Sci. Technol. Lett.

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Exoelectrogenic bacteria (EEB) play important roles in biogeochemical cycling, environmental remediation, wastewater treatment, and bioenergy recovery. Methods for effectively and rapidly probing the abundance of EEB in environments are highly desirable. In this work, a novel approach that couples WO3 nanoclusters and the most probable number (MPN) method for rapid detection and enumeration of EEB was developed. This WO3–MPN approach allowed rapid and reliable estimation of the population size of two typical EEB, Shewanella oneidensis MR-1 and Geobacter sulfurreducens DL-1. In addition, it was successfully applied to detect and count EEB in environmental samples from the sediments of a freshwater lake (9.9 × 104 to 4.1 × 106 cells/g of dry sediment) and engineered samples of a municipal wastewater treatment plant (1.0 × 103 to 7.5 × 105 cells/mL). This work may facilitate better identification and practical applications of EEB in natural and engineered environments.

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Section: Environmental Science and Technology Letterscontrasting

confidence: 58%

“…was also strongly affected by the phosphorus level. 25 This result implies that abundant EEB might inhabit in eutrophic freshwater lakes.…”

Section: ■ Results and Discussionmentioning

confidence: 93%

Rapid Detection and Enumeration of Exoelectrogenic Bacteria in Lake Sediments and a Wastewater Treatment Plant Using a Coupled WO₃ Nanoclusters and Most Probable Number Method

Yang

Cheng

Zhang

et al. 2016

Environ. Sci. Technol. Lett.

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“…Surface sediments were collected in October 2018 from Lake Dianchi, a eutrophic lake located in Kunming, P. R. China (24°40′–25°02′N, 102°36′–103°40′E), using the method described by Tian et al (2015). Surface sediments were sealed in sterile plastic bags, transported to the laboratory, homogenized and then used for experiments.…”

Section: Methodsmentioning

confidence: 99%

Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes

Hong

Shu

et al. 2019

AMB Expr

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The responses of denitrifiers and denitrification ability to dissolved oxygen (DO) concent in different layers of surface lake sediments are still poorly understood. Here, the optimal denitrification condition was constructed based on response surface methodology (RSM) to analyze the denitrification characteristics of surface sediments. The aerobic zone (AEZ), hypoxic zone (HYZ), up-anoxic zone (ANZ-1) and sub-anoxic zone (ANZ-2) were partitioned based on the oxygen contents, and sediments were collected using a customized-designed sub-millimeter scale sampling device. Integrated real-time quantitative PCR, Illumina Miseq-based sequencing and denitrifying enzyme activities analysis revealed that denitrification characteristics varied among different DO layers. Among the four layers, the DNA abundance and RNA expression levels of norB , nirS and nosZ were the highest at the aerobic layer, hypoxic layer and up-axoic layer, respectively. The hypoxia and up-anaerobic layer were the active nitrogen removal layers, since these two layers displayed the highest DNA abundance, RNA expression level and enzyme activities of denitrification functional genes. The abundance of major denitrifying bacteria showed significant differences among layers, with Azoarcus , Pseudogulbenkiania and Rhizobium identified as the main nirS , nirK and nosZ -based denitrifiers. Pearson’s correlation revealed that the response of denitrifiers to environmental factors differed greatly among DO layers. Furthermore, napA showed higher DNA abundance and RNA expression level in the aerobic and hypoxic layers than anaerobic layers, indicating that aerobic denitrifiers might play important roles at these layers. Electronic supplementary material The online version of this article (10.1186/s13568-019-0855-9) contains supplementary material, which is available to authorized users.

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“…Surface sediment was collected in May 2018 from Dianchi Lake, located in Kunming, China (102°38ʹ0.84ʹʹE, 24°58ʹ41.61ʹʹN) using specifi c sampling methods described previously by Tian et al (2015). Surface sediment cores were sampled to a depth of 20 mm and carefully transferred to PVC cylinders in situ (30 mm diameter × 110 mm high) before being subject to laboratory microcosm incubations.…”

Section: Experimental Designmentioning

confidence: 99%

Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface

et al. 2019

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At present, the understanding of the dynamics of denitrifi ers at diff erent dissolved oxygen (DO) layers under organic carbon consumption within the surface sediments remains inadequate. In this study, high-throughput sequencing and quantitative PCR targeting nirS gene were used to analyze the denitrifi er abundance dynamics, community composition, and structure for aerobic (DO 0.5-6.9 mg/L), hypoxic-anoxic (DO 0-0.5 mg/L), and anoxic (DO 0 mg/L) layers in surface sediments under organic carbon consumption. Based on the analysis of nirS gene abundance, the values of denitrifying bacteria decreased with organic carbon consumption at diff erent DO layers. When the bacterial species abundance at the genus level were compared between the high-carbon and low-carbon sediments, there was signifi cant increase in 6 out of 36, 7 out of 36 and 6 out of 36 genera respectively for the aerobic, hypoxic-anoxic and anoxic layers. On the other hand, 14 out of 36, 9 out of 36 and 15 out of 36 genera showed signifi cant decrease in bacterial species abundance respectively for the aerobic, hypoxic-anoxic and anoxic layers. Additionally, 14 out of 36, 20 out of 36, and 15 out of 36 genera had no change in bacterial species abundance respectively for the aerobic, hypoxic-anoxic, and anoxic layers. This indicates that the carbon utilization ability of diff erent denitrifi ers on each DO layers was generally diff erent from each other. Diversity of denitrifying bacteria also presented signifi cant diff erences in diff erent DO layers between the high-and low-carbon content sediment layers. Moreover, under the high-carbon and low-carbon content, the abundance of nirS gene showed a high peak within the hypoxic-anoxic regions, suggesting that this region might be the main distribution area for the denitrifying bacteria within the surface sediments. Furthermore, community of unique denitrifi ers occurred in diff erent DO layers and the adaptive changes of the denitrifi er community followed the organic carbon consumption.

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Vertical distribution of Fe and Fe(III)-reducing bacteria in the sediments of Lake Donghu, China

Cited by 12 publications

References 59 publications

Rapid Detection and Enumeration of Exoelectrogenic Bacteria in Lake Sediments and a Wastewater Treatment Plant Using a Coupled WO₃ Nanoclusters and Most Probable Number Method

Rapid Detection and Enumeration of Exoelectrogenic Bacteria in Lake Sediments and a Wastewater Treatment Plant Using a Coupled WO₃ Nanoclusters and Most Probable Number Method

Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes

Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface

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Vertical distribution of Fe and Fe(III)-reducing bacteria in the sediments of Lake Donghu, China

Cited by 12 publications

References 59 publications

Rapid Detection and Enumeration of Exoelectrogenic Bacteria in Lake Sediments and a Wastewater Treatment Plant Using a Coupled WO3 Nanoclusters and Most Probable Number Method

Rapid Detection and Enumeration of Exoelectrogenic Bacteria in Lake Sediments and a Wastewater Treatment Plant Using a Coupled WO3 Nanoclusters and Most Probable Number Method

Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes

Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface

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Product

Resources

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Rapid Detection and Enumeration of Exoelectrogenic Bacteria in Lake Sediments and a Wastewater Treatment Plant Using a Coupled WO₃ Nanoclusters and Most Probable Number Method

Rapid Detection and Enumeration of Exoelectrogenic Bacteria in Lake Sediments and a Wastewater Treatment Plant Using a Coupled WO₃ Nanoclusters and Most Probable Number Method