Vertical distribution of denitrification potential, denitrifying bacteria, and benzoate utilization in intertidal microbial mat communities

Golet, D. S.; Ward, Bettie

doi:10.1007/s002489900184

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…To our knowledge, a study combining electrode measurements and 15 N isotope techniques with new molecular approaches for determining denitri¢cation in aquatic habitats has not yet been thoroughly performed. Results for marine sediments, in which nitrate supplementation was required to detect potential denitri¢cation activity as assayed by the acetylene inhibition technique [158], are consistent with observations in soils (above). High abundances of nirS were detected by DNA hybridization in these sediments, but individual strains of denitrifying bacteria, enumerated by immuno-£uorescence, were present at very low abundance.…”

Section: Denitri¢cation In Aquatic Habitatssupporting

confidence: 83%

“…These results of DNAD NA hybridization experiments (dot-blot analyses) with these gene probes were interpreted as indicating that culturable denitrifying bacteria are enriched in the upper soil layer and in the vicinity of plant roots. Using a similar hybridization approach [137,158], high abundances of putative denitrifying bacteria were detected in marine water columns and sediment environments. Since these initial reports, many further sequences of denitri¢cation genes have been published (Table 1).…”

Section: Detection Of Denitrifying Bacteria In Soilsmentioning

confidence: 99%

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Molecular analysis of ammonia oxidation and denitrification in natural environments

Bothe¹,

Jost²,

Schloter³

et al. 2000

FEMS Microbiol Rev

248

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This review summarizes aspects of the current knowledge about the ecology of ammonia-oxidizing and denitrifying bacteria. The development of molecular techniques has contributed enormously to the rapid recent progress in the field. Different techniques for doing so are discussed. The characterization of ammonia-oxidizing and -denitrifying bacteria by sequencing the genes encoding 16S rRNA and functional proteins opened the possibility of constructing specific probes. It is now possible to monitor the occurrence of a particular species of these bacteria in any habitat and to get an estimate of the relative abundance of different types, even if they are not culturable as yet. These data indicate that the composition of nitrifying and denitrifying communities is complex and apparently subject to large fluctuations, both in time and in space. More attempts are needed to enrich and isolate those bacteria which dominate the processes, and to characterize them by a combination of physiological, biochemical and molecular techniques. While PCR and probing with nucleotides or antibodies are primarily used to study the structure of nitrifying and denitrifying communities, studies of their function in natural habitats, which require quantification at the transcriptional level, are currently not possible.

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Section: Denitri¢cation In Aquatic Habitatssupporting

confidence: 83%

Section: Detection Of Denitrifying Bacteria In Soilsmentioning

confidence: 99%

Molecular analysis of ammonia oxidation and denitrification in natural environments

Bothe¹,

Jost²,

Schloter³

et al. 2000

FEMS Microbiol Rev

248

View full text Add to dashboard Cite

show abstract

“…Central research thus remains to be performed on the molecular biology, physiology, and ecology of anammox. The highly expansive field of molecular technologies is, however, largely outside the scope of the present contribution, and for further information we refer the interested reader to current publications on the subject (e.g., Allen et al, 2001;Caffrey et al, 2003;Golet and Ward, 2001).…”

Section: Outlook For Future Researchmentioning

confidence: 99%

Nitrogen removal in marine environments: recent findings and future research challenges

et al. 2005

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International audienceRespiratory reduction of nitrate (denitrification) is recognized as the most important process converting biologically available (fixed) nitrogen to N2. In current N cycle models, a major proportion of global marine denitrification (50â70%) is assumed to take place on the sea floor, particularly in organic rich continental margin sediments. Recent observations indicate that present conceptual views of denitrification and pathways of nitrate reduction and N2 formation are incomplete. Alternative N cycle pathways, particularly in sediments, include anaerobic ammonium oxidation to nitrite, nitrate and N2 by Mn-oxides, and anaerobic ammonium oxidation coupled to nitrite reduction and subsequent N2 mobilization. The discovery of new links and feedback mechanisms between the redox cycles of, e.g., C, N, S, Mn and Fe casts doubt on the present general understanding of the global N cycle. Recent models of the oceanic N budget indicate that total inputs are significantly smaller than estimated fixed N removal. The occurrence of alternative N reaction pathways further exacerbates the apparent imbalance as they introduce additional routes of N removal. In this contribution, we give a brief historical background of the conceptual understanding of N cycling in marine ecosystems, emphasizing pathways of aerobic and anaerobic N mineralization in marine sediments, and the implications of recently recognized metabolic pathways for N removal in marine environments

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“…Desnues et al (2007) showed that nir was only distributed in the surface layer in winter and summer. Golet and Ward (2001) found that the absolute abundance of Nir was the highest in the depth of 9 ∼ 12 mm, indicating that the denitrification process caused by heterotrophs could be the most powerful in the surface sediment. Fan et al (2015) analysed nirK and nirS genes based on GeoChip, and the results showed that the abundance of both nirK and nirS was highest in the intertidal edge, confirming our simulations.…”

Section: Discussionmentioning

confidence: 99%

Zonations and oscillations via heterotrophic processes in tidal unvegetated aquifers

Yao

Kong

Zhang

et al. 2022

Hydrological Processes

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Global-change drivers that alter tidal amplitude or sea level could directly impact coastal aquifers' seawater-microbe interactions. The semidiurnal or diurnal tide can have consequences for chemical redistribution, because it deepens oxygen and nutrients penetration depth, thereby increasing O 2 depletion via microbial respiration in sediments during groundwater circulation. However, chemical and microbial distributions in tidal aquifers remain unclear, in deference to their link with depth and lateral location. By combining the microbes-accumulated equation with the traditional groundwater flow model in coastal aquifers, we explicitly unravelled the heterotrophic zonation, the salinity zonation, and the oxic zonation of tidal aquifers. According to the model estimates, salt penetrates deeper than reactive chemicals, and the heterotrophic high biomass zone is presumably 2-3 m deep. Such depth of heterotrophic distribution is 2-3 orders of magnitude higher than phototrophic distribution.Additionally, heterotrophic biomass can exhibit faint oscillations, even under the tide forces, whereas dissolved chemicals fluctuate significantly with tidal pumps. Due to

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Vertical distribution of denitrification potential, denitrifying bacteria, and benzoate utilization in intertidal microbial mat communities

Cited by 10 publications

References 44 publications

Molecular analysis of ammonia oxidation and denitrification in natural environments

Molecular analysis of ammonia oxidation and denitrification in natural environments

Nitrogen removal in marine environments: recent findings and future research challenges

Zonations and oscillations via heterotrophic processes in tidal unvegetated aquifers

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