Vertical Distribution of Bacteria in Various Lakes of East Java, Indonesia

Prabaningtyas, Sitoresmi; Witjoro, Agung; Suarsini, Endang; Aridowi, Dhiyaudin; Nafizatuzamrudah,; Aini, Afifah Nur; Purnomo, Adji; Permana, Yuniar Indra; Meirinda,; Aribah, Dina; Yanis,

doi:10.1088/1742-6596/1093/1/012017

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…The environment affects the lives of these bacteria, especially those that live in water. According to the previous study Prabaningtyas et al (2018), several water conditions in this lake, such as pH about 6.8-9.4, dissolved oxygen (DO), were between 4.2 and 11.6 mg/L, and salinity was around 0.00%-0.4%, which are estimated to have nitrogen-fixing bacteria. The range of pH about 6-9 is feasible for ammonium formation (Luo et al 2015).…”

Section: Ammonium Semi-quantitative and Quantitative Assaymentioning

confidence: 85%

“…Each lake has different conditions which are influenced by abiotic factors. The abiotic factors were measured in five stations from each lake, Ranu Regulo consists of DO around 8-11.6 mg/L, pH 8.65-9.4, Salinity 0-0.3, and Transparency 75-80 cm; Ranu Pani consists of DO 4.2-11.6, pH 6.83-7.3, Salinity 0.2-0.4, Transparency 50-100; Ranu Grati consists of DO 7.1-8.5, pH 7.1-8.82, Salinity 0, Transparency 100-110 cm and the abiotic factors from Telaga Ngebel consist of DO 2.5-8.5, pH 6.98-7.69, Salinity 0.1-0.2, and Transparency 383-676 cm (Prabaningtyas et al 2018).…”

Section: Screening Of Non-symbiotic Nitrogen-fixing Bacteriamentioning

confidence: 99%

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Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia

et al. 2022

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Abstract. Nafisah W, Prabaningtyas S, Witjoro A, Saptawati RT, Rodiansyah A. 2022. Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia. Biodiversitas 23: 1752-1758. Potential microorganisms, mainly nitrogen-fixing bacteria spread in almost lake areas in East Java. The biogeochemical processes that occur between biotic-biotic or biotic-abiotic factors can accelerate the growth of autotrophic microorganisms in waters as like microalgae. Non-symbiotic nitrogen-fixing bacteria in the water can supply nitrogen to support the growth of microalgae. This study is aimed to explore and calculate the ammonium concentration produced by non-symbiotic nitrogen-fixing bacteria that isolated from several lakes in East Java. The highest ammonium producing bacteria were characterized based on morphology, physiology, and genetic with 16S rRNA gene sequencing. The bacterial isolates were screened with a nitrogen-free bromothymol blue medium to select bacterial isolates capable of fixing nitrogen. The content of NH3/NH4 was produced by isolates of nitrogen-fixing bacteria was measured by using the prodac test kit NH3/NH4. The ammonium concentration was calculated with Nessler’s reagent by using the spectrophotometry method at a wavelength of 425 nm. The result was obtained 31 isolates of non-symbiotic nitrogen-fixing bacteria. The bacterial isolate B2 exhibited highest activity of fixing nitrogen that produced ammonium (14.46 mg/L) among the other screened bacteria. The morphological and physiological characterization from that isolate was identified as genus Bacillus, whereas the genetic characterization similar to Bacillus paramycoides, that included in the Bacillus cereus group.

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Section: Ammonium Semi-quantitative and Quantitative Assaymentioning

confidence: 85%

Section: Screening Of Non-symbiotic Nitrogen-fixing Bacteriamentioning

confidence: 99%

Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia

et al. 2022

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“…Ranu Grati is a large lake in East Java, with various activities around the lake, which can cause the community structure to change. The preliminary study result show that these lakes include eutrophic lakes with a secchi disk depth (brightness) of 75-110 cm, medium microalgae diversity index, moderate dominance, and abundance of high microalgae (Prabaningtyas et al 2018). In lake water, bacteria and microalgae interact with each other (Kazamia et al 2014).…”

Section: Introductionmentioning

confidence: 97%

Exploration of vitamin B12-producing bacteria from Indonesia Eutrophic Lake: A new strategy to improve microalgae biomass production

et al. 2021

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Abstract. Prabaningtyas S, Ardyati T, Suharjono, Retnaningdyah C. 2021. Exploration of vitamin B12-producing bacteria from Indonesia Eutrophic Lake: A new strategy to improve microalgae biomass production. Biodiversitas 22: 4538-4544. Biofuel producing autotrophs and heterotrophs organisms are mostly found in freshwaters such as lakes. Preliminary research showed that Ranu Grati has a high diversity of microalgae and bacteria. Microalgae are known as one source of biofuels due to the high content of lipid. The growth of microalgae is strongly influenced by vitamin B12, which is synthesized by bacteria. The role of vitamin B12 in the metabolism of algae is mainly as a co-enzyme for vitamin B12-dependent methionine synthase. The purpose of this research was to observe the bacterial diversity based on NGS analysis, to explore vitamin B12 produced by bacteria from Ranu Grati lake, and also to identify the highest potency of vitamin B12-producing bacteria based on 16S rRNA gene sequencing. The study was carried out by taking a water sample at a depth of 50 cm at five stations. Isolation of total bacterial DNA was carried out using FastDNA Spin Kit for Soil. The metagenomic method of Next Generation Sequencing (NGS) was used as an initial study of bacterial diversity. Vitamin B12-producing bacteria was isolated using PYBG agar (phytone peptone, trypticase peptone, lab-lemco powder, bacto yeast extract, glucose, agar). Isolated bacteria were screened for production of vitamin B12. The potential isolates were identified base on 16S rRNA gene sequence similarity. The results of the metagenomic study showed that the genus of potential bacteria producing vitamin B12 included Bacillus, Pseudomonas, Propionibacterium, Enterobacter, Escherichia, Acinetobacter, and Flavobacterium. The results of screening with PYBG media obtained 30 isolates of vitamin B12 producing bacteria. Ten efficient vitamin B12-producing isolates were identified as Lysinibacillus fusiformis (isolate G2V1), Bacillus cereus group (isolate G2V25, G2V24, G2V13, G2V9, G2V8), Alcaligenes faecalis (isolate G2V22), Delftia acidovorans (isolate G2V14A) and the genus Delftia (isolate G2V19). The isolate G2V1 (Lysinibacillus fusiformis) was the highest producer of vitamin B12 which was able to produce 33,783 ug/mL of vitamin B12 at 4 days incubation time.

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“…The previous research was found 96 bacterial isolates from Ranu Pani, Ranu Regulo, Ranu Grati, and Telaga Ngebel which have multiple potencies as follows, 40 isolates have the potency to produce IAA, 31 phosphate solubilizing bacterias, two dominant bacterias from Ranu Pani and Ranu Grati can promote microalgae growth (Prabaningtyas et al 2018a(Prabaningtyas et al , 2018b, 47 cellulolytic bacterias (Ardilla 2018), and 53 amylolytic bacterias (Basitoh 2018). Identification of bacteria that have the highest potential for producing IAA and solubilizing phosphate yet not been done.…”

Section: Introductionmentioning

confidence: 99%

Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis

et al. 2021

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Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.

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Vertical Distribution of Bacteria in Various Lakes of East Java, Indonesia

Cited by 5 publications

References 6 publications

Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia

Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia

Exploration of vitamin B12-producing bacteria from Indonesia Eutrophic Lake: A new strategy to improve microalgae biomass production

Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis

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