Vertical distribution and temporal variation of marine planktonic archaea in the Gerlache Strait, Antarctica, during early spring

Massana, Ramón; Taylor, Lance T.; Murray, Alison E.; Wu, Ke; Jeffrey, Wade H.; DeLong, Edward F.

doi:10.4319/lo.1998.43.4.0607

Cited by 113 publications

(122 citation statements)

References 35 publications

(37 reference statements)

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“…Seawater was first passed through in-line polycarbonate filter holders with 1.0 pm glass fiber filters (MSI), then cells were collected on 0.22 pm Sterivex filters (Millipore). Though picoplankton > 1.0 pm would have been excluded from our analysis, Massana et al (1998) showed that the majority of archaeal cells reside in the <0.8 pm fraction in Antarctic waters. Lysis buffer (1.8 ml; 40 mM EDTA, 50 mM Tris-HC1, 0.75 M sucrose) was added to the Sterivex filter units after filtration and the samples were stored frozen at -20°C until nucleic acid extraction.…”

Section: Methodsmentioning

confidence: 99%

A time series assessment of planktonic archaeal variability in the Santa Barbara Channel

Murray¹,

Blakis²,

Massana³

et al. 1999

Aquat. Microb. Ecol.

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Although detailed temporal studies of marine planktonic cyanobacteria have been reported, relatively little is known about variation of other marine picoplankton groups on time scales on the order of months to years. In this study, we followed vanation in relative abundance of planktonic archaeal rRNA at 5 depths in the upper 300 m of the Santa Barbara Channel (SBC) over 32 mo. Small subunit rRNA targeted oligonucleotide probes were used to quantify archaeal, bacterial, and eucaryal rRNA relative abundance. Archaea subgroups were quantified using probes specific to 2 planktonic archaeal groups, termed G1 and G11 The archaeal rRNA signal in the upper 20 m was characterized by intermittent 'blooms' that coincided with increases in the relatlvc ab.undance of GII rRNA as well as decreases in chlorophyll a (chl a). At greater depths, archaeal rRNA abundance was consistently elevated, approaching bacterial rRNA abundance. The G1 rRNA accounted for the majority of the deeper archaeal rRNA signal throughout the time series. Consistent with a previous report, the 2 groups of archaea had maximal rRNA abundance at different depths. The lnajolity of the variability in the G1 archaeal slgnal in the upper 75 m (77 %) could be attributed to a positive relat~onship with nutrients and negative relationships with prokaryotic abundance, chl a, and temperature; although, at most, 41% of the variability at each depth could be explained. Only 21 % of the variability in the G11 rRNA signal could be explained by the variables included in the model. Leucine incorporation rates and, to a lesser extent, prokaryote abundance, were highly correlated with chl a and POC levels. Only weak relationships were observed between prokaryote abundance or leucine incorporation and incredses in temperature. The results of linear regressions between prokaryote abundance and leucine incorporation suggest that top-down ecological controls may regulate prokaryotic assemblages in this dynamic coastal system. The distribution of the 2 archaeal groups suggested that they responded independently to environmental conditions, are physiologically different, and likely participate in different environmental processes.

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Section: Methodsmentioning

confidence: 99%

A time series assessment of planktonic archaeal variability in the Santa Barbara Channel

Murray¹,

Blakis²,

Massana³

et al. 1999

Aquat. Microb. Ecol.

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“…Some studies have shown a clear link between the distribution of the major prokaryotic groups to bulk (Massana et al 1998) and group-specific prokaryotic activity in the dark ocean (Teira et al 2004, 2006b, Herndl et al 2005. This relationship might be more evident in regions with strong environmental gradients and relatively high picoplankton activity.…”

Section: Introductionmentioning

confidence: 97%

“…Archaea are widespread among marine prokaryotic plankton (De Long et al 1994, Massana et al 1998. Quantitative studies using FISH have shown the dominance of Crenarchaeota in meso-and bathypelagic waters, whereas Euryarchaeota were thought to comprise less than 5 to 8% of total picoplankton abundance in deep waters of the Pacific and the Antarctic Oceans (Karner et al 2001, Church et al 2003.…”

Section: Introductionmentioning

confidence: 99%

Strong coastocean and surfacedepth gradients in prokaryotic assemblage structure and activity in a coastal transition zone region

Baltar

Arı́stegui²,

Gasol³

et al. 2007

Aquat. Microb. Ecol.

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The distribution of marine Crenarchaeota Group I, marine Euryarchaeota Group II and some major groups of Bacteria (SAR 11, Roseobacter, Gammaproteobacteria and Bacteroidetes) was investigated in the North Atlantic water column (surface to 2000 m depth) along a transect from the coastal waters of the NW African upwelling to the offshore waters of the Canary Coastal Transition Zone (CTZ). Catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) was used to describe the prokaryotic assemblages. Bulk picoplankton abundance and leucine incorporation were determined. Pronounced changes in prokaryotic assemblage composition were observed from the coast to the open ocean and at the deep chlorophyll maximum (DCM) with decreasing bulk heterotrophic activity. All bacterial groups decreased in absolute abundances from the coast to the open ocean; both archaeal groups increased towards the open ocean. Prokaryotic abundance and activity decreased 2 and 3 orders of magnitude, respectively, from the surface to 2000 m. Prokaryotic growth rates were high in the mesopelagic zone (~0.13 d ), compared to other reports from the central North Atlantic. SAR11 in total picoplankton abundance decreased from 42% in the DCM to 4% at 2000 m, while marine Crenarchaeota Group I increased from 1% in the DCM to 39% in the oxygen minimum layer. A clear influence of the different intermediate water masses was observed on the bulk heterotrophic picoplankton activity, with lower leucine incorporation rates corresponding to layers where patches of Antarctic Intermediate Water were detected. Coast -ocean and surface-depth gradients in bulk prokaryotic abundance and production and assemblage composition were comparable to changes observed in basin-scale studies, pinpointing the CTZs as regions of strong variability in microbial diversity and metabolism.

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“…Initial observations in the Southern Ocean found that archaeal ribonucleic acids accounted for 16-34% of the bulk community rRNA obtained from surface seawater in the nearshore waters off Anvers Island, Antarctica, in the late austral winter (DeLong et al 1994). Subsequent studies in this region confirmed the presence of Archaea and noted an apparent seasonality in the relative amount of archaeal rRNA in the surface waters Massana et al 1998).…”

mentioning

confidence: 99%

Abundance and distribution of planktonic Archaea and Bacteria in the waters west of the Antarctic Peninsula

Church

DeLong

Ducklow

et al. 2003

Limnology & Oceanography

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Polyribonucleotide probes targeting planktonic archaeal (Group I and II) and bacterial rRNA revealed that Archaea comprised a significant fraction of total prokaryote cell abundance in the marine waters west of the Antarctic Peninsula. Determinations of Archaea and Bacteria cell abundances were made during two research cruises to the Palmer Long-Term Ecological Research region during the austral winter and summer of 1999. During the austral summer, surface water abundances of Group I (GI) Archaea were generally low, averaging 4.7 ϫ 10 3 cells ml Ϫ1and accounting for 1% of the total picoplankton assemblage. The abundance of GI Archaea increased significantly with depth, averaging 2.1 ϫ 10 4 cells ml Ϫ1 and comprising 9-39% of the total picoplankton abundance in the meso-(150-1,000 m) and bathypelagic (1,000-3,500 m) circumpolar deep water (CDW). Relative to summertime distributions, GI cells were more evenly distributed throughout the water column during the winter, averaging 10% of the picoplankton in the surface waters and 13% in the CDW. Surface water GI abundance increased 44% between the summer and winter, coincident with a fivefold decrease in GI abundance in the deeper waters. The abundance of Group II (GII) Archaea was persistently Ͻ2% of the total picoplankton throughout the water column in both summer and winter. Bacterial abundance was greatest in the upper water column (0-100 m) during the summer, averaging 3.9 ϫ 10 5 cells ml Ϫ1 and comprised 89% of the total picoplankton assemblage. Generally, GI Archaea varied seasonally in the deeper waters, whereas bacterial abundance varied more in the upper waters. The observed variability in bacterial and archaeal abundance suggests that these two groups of marine picoplankton are dynamic components of Southern Ocean microbial food webs.Marine picoplankton are arguably the most poorly understood members of the marine plankton community, mainly because of poor cultivation success. Continuing development of cultivation Rappé et al.

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Vertical distribution and temporal variation of marine planktonic archaea in the Gerlache Strait, Antarctica, during early spring

Cited by 113 publications

References 35 publications

A time series assessment of planktonic archaeal variability in the Santa Barbara Channel

A time series assessment of planktonic archaeal variability in the Santa Barbara Channel

Strong coastocean and surfacedepth gradients in prokaryotic assemblage structure and activity in a coastal transition zone region

Abundance and distribution of planktonic Archaea and Bacteria in the waters west of the Antarctic Peninsula

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Vertical distribution and temporal variation of marine planktonic archaea in the Gerlache Strait, Antarctica, during early spring

Cited by 113 publications

References 35 publications

A time series assessment of planktonic archaeal variability in the Santa Barbara Channel

A time series assessment of planktonic archaeal variability in the Santa Barbara Channel

Strong coastocean and surfacedepth gradients in prokaryotic assemblage structure and activity in a coastal transition zone region

Abundance and distribution of planktonic Archaea and Bacteria in the waters west of the Antarctic Peninsula

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Strong coastocean and surfacedepth gradients in prokaryotic assemblage structure and activity in a coastal transition zone region