Vertical capacitance aptasensors for real-time monitoring of bacterial growth and antibiotic susceptibility in blood

Song, Jun; Lee, Sun Mi; Yong, Dongeun; Lee, Kyo Seok; Shin, Jeon Soo; Yoo, Kyung Hwa

doi:10.1016/j.bios.2019.111623

Cited by 10 publications

(10 citation statements)

References 30 publications

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“…Although this model represents a fast, label-free, sensitive, and nondestructive method to characterize biofilms in terms of attachment and maturation, it requires important technical and theoretical considerations for appropriate data interpretation. Song et al (2019) developed an aptamer-functionalized sensor to analyze the biofilm formation in blood samples. In general, if bacteria were present in the sample, they would become attached to the sensor, affecting the electrical properties.…”

Section: Ll Open Accessmentioning

confidence: 99%

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Mimicking biofilm formation and development: Recent progress in in vitro and in vivo biofilm models

et al. 2021

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Biofilm formation in living organisms is associated to tissue and implant infections, and it has also been linked to the contribution of antibiotic resistance. Thus, understanding biofilm development and being able to mimic such processes is vital for the successful development of antibiofilm treatments and therapies. Several decades of research have contributed to building the foundation for developing in vitro and in vivo biofilm models. However, no such thing as an ''all fit'' in vitro or in vivo biofilm models is currently available. In this review, in addition to presenting an updated overview of biofilm formation, we critically revise recent approaches for the improvement of in vitro and in vivo biofilm models.

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Section: Ll Open Accessmentioning

confidence: 99%

“… Song et al. (2019) developed an aptamer-functionalized sensor to analyze the biofilm formation in blood samples.…”

Section: In Vitro Models For Biofilm Assessmentmentioning

confidence: 99%

Mimicking biofilm formation and development: Recent progress in in vitro and in vivo biofilm models

et al. 2021

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“…Patients' survival could be highly improved if they received timely and effective antibiotic treatment, but antimicrobial susceptibility test (AST) results usually require more than three days with conventional methods [80][81][82]. In addition to the quantitative and qualitative detection of bacteria, the aptamer sensor can monitor the real-time growth of bacteria and biofilm, which continuously and timely evaluate the internal infections and the effectiveness of antibiotics [46,47]. For instance, Song et al developed a vertical capacitance aptamer-functionalized sensor (Fig.…”

Section: Real-time Monitoring Of Bacterial Growth and Astmentioning

confidence: 99%

“…6 The scheme of GN6 ELAA and the specificity of GN6 to some OMVs [49] Fig. 7 Scheme of vertical capacitance aptamer-functionalized sensor [46] more expensive, it is significant to save patients suffering from sepsis.…”

Section: Real-time Monitoring Of Bacterial Growth and Astmentioning

confidence: 99%

Aptamer-based biosensors for the diagnosis of sepsis

Liu

Han

et al. 2021

J Nanobiotechnol

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Sepsis, the syndrome of infection complicated by acute organ dysfunction, is a serious and growing global problem, which not only leads to enormous economic losses but also becomes one of the leading causes of mortality in the intensive care unit. The detection of sepsis-related pathogens and biomarkers in the early stage plays a critical role in selecting appropriate antibiotics or other drugs, thereby preventing the emergence of dangerous phases and saving human lives. There are numerous demerits in conventional detection strategies, such as high cost, low efficiency, as well as lacking of sensitivity and selectivity. Recently, the aptamer-based biosensor is an emerging strategy for reasonable sepsis diagnosis because of its accessibility, rapidity, and stability. In this review, we first introduce the screening of suitable aptamer. Further, recent advances of aptamer-based biosensors in the detection of bacteria and biomarkers for the diagnosis of sepsis are summarized. Finally, the review proposes a brief forecast of challenges and future directions with highly promising aptamer-based biosensors.

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“…Bacteria act as dielectric particles; thus, if bacteria are bound to the sensor surface via the aptamer, the capacitance changes. As a result, bacterial activities, such as growth and death, can be monitored in real-time by measuring the capacitance change, allowing more rapid AST 23 , 24 . For AST of clinical samples, it is necessary to simultaneously test various antimicrobial agents at different concentrations; therefore, we fabricated sensor arrays consisting of 60 sensors.…”

Section: Introductionmentioning

confidence: 99%

Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates

Lee

Oh³

et al. 2020

Sci Rep

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capacitance changes. As a result, bacterial activities, such as growth and death, can be monitored in real-time by measuring the capacitance change, allowing more rapid AST 23,24. For AST of clinical samples, it is necessary to simultaneously test various antimicrobial agents at different concentrations; therefore, we fabricated sensor arrays consisting of 60 sensors. Among the 60 sensors, two and three sensors were used for negative and positive controls, respectively, and the other 55 sensors were used to measure the antibiotic susceptibility to 11 antibiotics at five different concentrations (see Supplementary Table S1). To evaluate the performance of the eAST system, 30 clinical strains, most of which are frequently found in sepsis, were tested (Table 1). The categorical agreement between the eAST system and gold standard broth microdilution (BMD) test was estimated to be 97%, meeting the requirements of the United States Food and Drug Administration (FDA). Materials and methods fabrication of eAST chip. The eAST chip, consisting of 60 sensors, was fabricated on a glass substrate (Fig. 1). Each sensor had interdigitated Au electrodes with a width of 20 µm and spacing of 20 µm, which were patterned by conventional photolithography and lift-off techniques. For bacterial culture, a 300 μl-acrylic well (Nobel Biosciences Inc., Hwaseong-Si, South Korea) was attached to the array sensor using 3 M VHBTM tape. Next, the bacteria-aptamers were immobilized on the sensor surface.

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Vertical capacitance aptasensors for real-time monitoring of bacterial growth and antibiotic susceptibility in blood

Cited by 10 publications

References 30 publications

Mimicking biofilm formation and development: Recent progress in in vitro and in vivo biofilm models

Mimicking biofilm formation and development: Recent progress in in vitro and in vivo biofilm models

Aptamer-based biosensors for the diagnosis of sepsis

Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates

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