Vertebrate Membrane Proteins: Structure, Function, and Insights from Biophysical Approaches

Müller, Daniel J.; Wu, Nan; Palczewski, Krzysztof

doi:10.1124/pr.107.07111

Cited by 88 publications

(77 citation statements)

References 328 publications

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“…If 50% of the surface area of the ER corresponds to protein molecules, then there are 2.5 ϫ 10 6 proteins in the ER and 1.6 ϫ 10 8 phospholipids. Assuming that the average molecular mass of ER membrane proteins is 50 kDa, the protein/phospholipid ratio of the ER is ϳ0.8 g/mmol, corresponding to a weight ratio of ϳ1:1 (g/g), as generally expected for cellular membranes (25).…”

Section: Materials-egg Phosphatidylcholine Egg Phosphatidylglycerolmentioning

confidence: 96%

Reconstitution of Glucosylceramide Flip-Flop across Endoplasmic Reticulum

Chalat

Menon

Turan

et al. 2012

Journal of Biological Chemistry

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Background: Lipid flip-flop, a key feature of many glycolipid biosynthetic pathways, requires as yet unidentified flippase proteins. Results: Glucosylceramide flips slowly across protein-free vesicles but rapidly across vesicles reconstituted with ER membrane proteins. Conclusion: Glucosylceramide flipping is facilitated by ATP-independent ER phospholipid flippases. Significance: Defining how glucosylceramide is transported across membranes is critical for understanding the glycosphingolipid biosynthetic pathway.

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Section: Materials-egg Phosphatidylcholine Egg Phosphatidylglycerolmentioning

confidence: 96%

Reconstitution of Glucosylceramide Flip-Flop across Endoplasmic Reticulum

Chalat

Menon

Turan

et al. 2012

Journal of Biological Chemistry

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“…Determining the structure of target molecules is crucial for understanding their interactions with drug molecules [26] . Most of the targets are membrane proteins.…”

Section: High-resolution Imaging and Manipulation Of Individual Nativmentioning

confidence: 99%

“…In the past decade, AFM has emerged as a valuable addition to the pharmaceutical industry's toolkit [24] and has been demonstrated to be useful in assisting drug evaluations and assays (eg, drug-target interactions [25] , target molecules [26,27] and drug delivery system characterizations [28,29] ). The applications of AFM in pharmacology have provided novel fundamental insight for understanding drug actions [30] .…”

Section: Introductionmentioning

confidence: 99%

Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy

Liu

et al. 2015

Acta Pharmacol Sin

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Knowledge of the nanoscale changes that take place in individual cells in response to a drug is useful for understanding the drug action. However, due to the lack of adequate techniques, such knowledge was scarce until the advent of atomic force microscopy (AFM), which is a multifunctional tool for investigating cellular behavior with nanometer resolution under near-physiological conditions. In the past decade, researchers have applied AFM to monitor the morphological and mechanical dynamics of individual cells following drug stimulation, yielding considerable novel insight into how the drug molecules affect an individual cell at the nanoscale. In this article we summarize the representative applications of AFM in characterization of drug actions on cell membrane, including topographic imaging, elasticity measurements, molecular interaction quantification, native membrane protein imaging and manipulation, etc. The challenges that are hampering the further development of AFM for studies of cellular activities are aslo discussed.

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“…Typically, transmembrane proteins are comprised of hydrophobic cores with ionizable or charged residues at specific locations that are crucial for their appropriate functionality Muller et al (2008). G protein-coupled receptors (GPCRs) comprise a large protein family of transmembrane receptors that sense molecules outside the cell and activate the signal transduction pathways inside and regulate cellular responses Rosenbaum et al (2009).…”

Section: Study Of Membrane Proteins Using Protein Footprintingmentioning

confidence: 99%