Vertebrate histone gene transcription occurs from both DNA strands

Bruschi, Sam A.; Wells, J. R. E.

doi:10.1093/nar/9.7.1591

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…Cloned Gene Probes. Recombinant plasmids containing chicken histone genes that were used include pHU 4.8E (HI, H2A, and H2B) and pHU 2.6H (H3 and H4) (Sierra et al, 1982;Engel & Dodgson, 1981;Bruschi & Wells, 1981).…”

Section: Methodsmentioning

confidence: 99%

Down-regulation of cell growth and cell cycle regulated genes during chick osteoblast differentiation with the reciprocal expression of histone gene variants

Shalhoub¹,

Gerstenfeld²,

Collart³

et al. 1989

Biochemistry

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Expression of cell cycle (core and H1 histone) and cell growth (c-myc and c-fos) regulated genes was examined in primary cultures of chick calvarial osteoblasts during a developmental sequence associated with the progressive maturation of the osteoblast in a bonelike mineralized extracellular matrix. We have identified a transition point early in the developmental sequence which occurs when proliferation ceases and expression of genes related to the differentiated phenotype of osteoblasts is initiated. During this transition period, cellular levels of RNA transcripts from core and H1 histone genes and the c-myc and c-fos protooncogenes decrease in a parallel and coordinate manner. The decline in histone gene transcription that accompanies the loss of accumulated histone mRNA indicates that the downregulation of histone gene expression is at least, in part, transcriptionally mediated. In contrast, persistence of c-myc and c-fos transcription following completion of the proliferation period, when the mRNAs are no longer present at detectable levels, suggests that the initial downregulation of protooncogene expression is controlled at the level of messenger RNA stability. Thus, two types of signaling mechanisms are operative in the down-regulation of cell proliferation genes during osteoblast differentiation--one that impinges on regulatory sequences that influence the interactions of transcription factors with cis-acting promoter elements and a second that modulates messenger RNA turnover. Of significance, downregulation of the cell cycle regulated histone genes is accompanied by a reciprocal increase in the expression of a structurally distinct subset of the histone genes that are not coupled with DNA replication during the period of expression of osteoblast phenotype markers.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Methodsmentioning

confidence: 99%

Down-regulation of cell growth and cell cycle regulated genes during chick osteoblast differentiation with the reciprocal expression of histone gene variants

Shalhoub¹,

Gerstenfeld²,

Collart³

et al. 1989

Biochemistry

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“…The faster moving 816-bp fragment was obtained (Maxam and Gilbert, 1980) for redigestion with Avall, Hinfl or EcoRI followed by endlabelling. When strand separation was required prior to carrying out the sequencing reactions, poy(U,G) was used (see Bruschi and Wells, 1981) in ratios (w/v) of 2:1 to 8:1 [poly(U,G) to DNA] depending on the fragment. Rather than using agarose gels (Bruschi and Wells, 1981), 5% polyacrylamide gels were used (1:50 cross-linkage) made up with Tris/borate/EDTA.Na2 buffer.…”

Section: Methodsmentioning

confidence: 99%

Apparent relatedness of the main component of ovine 1.714 satellite DNA to bovine 1.715 satellite DNA.

Reisner¹,

Bucholtz²

1983

The EMBO Journal

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The nucleotide sequence of the principal component of ovine 1.714 g/cm3 satellite DNA was determined from a monomeric fragment inserted at the BamHI site of pBR322 and cloned in Escherichia coli strain RR1. The 816‐bp tandemly repeated sequence contains a number of small repeated sequences dispersed within it, one group of which forms a pentameric tandem repeat of a 13‐bp segment (positions 548‐612). A 20‐bp region (60‐79) shows an 85% homology with the reverse‐complement of the sequence from 455 through 474. There are two regions of 67 bp (75‐141) and 59 bp (755‐813) which show greater than 70% homology with regions of bovine 1.715 g/cm3 satellite DNA (1402 bp; positions 1218‐1284 and 1079‐1137, respectively) while a 31‐bp region (ovine 62‐92, bovine 133‐163) shows 80% homology. Quasi‐correlation coefficients (Qr) were determined using the triplet numbers of the sheep satellite versus all sequences in the National Biomedical Research Foundation and EMBL nucleotide sequence data bases. Qr equals 0.85 for ovine 1.714 g/cm3 satellite versus bovine 1.715 g/cm3 satellite. The next highest Qr for a bovine satellite segment was 0.58. Thus, the ovine 1.714 g/cm3 and bovine 1.715 g/cm3 satellite appear demonstrably related. Taking into account that sheep and cattle diverged 18‐20 million years ago, this suggests that the material may be functional and that its function is related to its sequence.

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Nucleic Acid-Associated Protein Genes

Dillon

1987

The Gene

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Vertebrate histone gene transcription occurs from both DNA strands

Cited by 4 publications

References 17 publications

Down-regulation of cell growth and cell cycle regulated genes during chick osteoblast differentiation with the reciprocal expression of histone gene variants

Down-regulation of cell growth and cell cycle regulated genes during chick osteoblast differentiation with the reciprocal expression of histone gene variants

Apparent relatedness of the main component of ovine 1.714 satellite DNA to bovine 1.715 satellite DNA.

Nucleic Acid-Associated Protein Genes

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