Vertebrate estrogen regulates the development of female characteristics in silkworm, Bombyx mori

Shen, Guanghui; Lin, Ying; Yang, Cong; Xing, Runmiao; Zhang, Haiyan; Chen, Enxiang; Han, Chaoshan; Liu, Hongling; Zhang, Weiwei; Xia, Qingyou

doi:10.1016/j.ygcen.2014.09.016

Cited by 12 publications

(10 citation statements)

References 42 publications

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“…It has been suggested that this difference is related to the effect of 17-β-estradiol in females, which inhibits AA oxidation (Tarnopolsky, 2008). Even though this is considered a vertebrate hormone, it has been found to cause the development of female characteristics in another moth species, the silkworm (Bombyx mori) (Shen et al, 2015). We suggest that the mechanism regulating the level at which AAs are oxidized could be conserved among taxa, and is related to cholesterol-derived estrogen-like hormones -a promising topic for future studies of comparative physiology and endocrinology.…”

Section: Discussionmentioning

confidence: 99%

Sex differences in the utilization of essential and non-essential amino acids in a Lepidoptera

Levin

McCue

Davidowitz

2017

Journal of Experimental Biology

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The different reproductive strategies of males and females underlie differences in behavior that may also lead to differences in nutrient use between the two sexes. We studied sex differences in the utilization of two essential amino acids (EAAs) and one non-essential amino acid (NEAA) by the Carolina sphinx moth (Manduca sexta). On day one post-eclosion from the pupae, adult male moths oxidized greater amounts of larva-derived AAs than females, and more nectarderived AAs after feeding. After 4 days of starvation, the opposite pattern was observed: adult females oxidized more larva-derived AAs than males. Adult males allocated comparatively small amounts of nectar-derived AAs to their first spermatophore, but this allocation increased substantially in the second and third spermatophores. Males allocated significantly more adult-derived AAs to their flight muscle than females. These outcomes indicate that adult male and female moths employ different strategies for allocation and oxidation of dietary AAs.

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Section: Discussionmentioning

confidence: 99%

Sex differences in the utilization of essential and non-essential amino acids in a Lepidoptera

Levin

McCue

Davidowitz

2017

Journal of Experimental Biology

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“…The fertilized egg extracts, irrespective of being from control or E 2 -treated silkworms, failed to establish quantitative differences of the proteins in question. Recent findings of Shen et al (2015) confirmed that estradiol treatment by injection or by feeding to male larvae in the final instar stage induced and stimulated male BmVg protein accumulation in hemolymph. They also showed that vertebrate E 2 induced Vg synthesis in a dosedependent fashion in males.…”

Section: Biological Role Of Vertebrate Hormones In Silkwormsmentioning

confidence: 68%

“…Therefore, it may be concluded that estradiol has a sex hormone-like function, which can induce and maintain female-typical BmVg protein levels in male silkworms. Transplantation of ovaries in male silkworms from 5th instar female silkworms showed further development and egg production in fewer numbers in the male, and also the ovaries contained BmVn (vitellin; Shen et al, 2015). Induction and maintenance of female-typical protein has been shown to be stimulated by exogenous estradiol (Das and Ray, 2014a).…”

Section: Biological Role Of Vertebrate Hormones In Silkwormsmentioning

confidence: 99%

Vertebrate hormones in insects: the role of estrogen in silkworm – a review

Das¹

2016

Turk J Zool

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“…Total RNA was extracted from fat bodies using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and was treated with DNase; first‐strand cDNA was generated using Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to the manufacturer's protocol. RT‐PCR and qRT‐PCR for detection of BmVg [Nucleotide sequence of mRNA (NM): 001043844.1] and BmERR were performed as previously described (Shen et al ., ). BmERR , BmEcR (NM: 001043866.2) and BmUsp (NM: 001044005.1) were amplified under the following conditions: 94 °C for 5 min; 35 cycles of 94 °C for 30 s, 60 °C for 90 s and 72 °C for 30 s; and 72 °C for 10 min.…”

Section: Methodsmentioning

confidence: 97%

Oestrogen‐related receptor reduces vitellogenin expression by crosstalk with the ecdysone receptor pathway in female silkworm, Bombyx mori

Shen

Han

et al. 2018

Insect Molecular Biology

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Oestrogen-related receptor (ERR) is involved in oestrogen receptor (ER) signalling pathways owing to its similarity to ER in terms of domain structure and co-activator and response elements. Although insects lack ER, they harbour an ERR gene that is thought to modulate metabolism and energy conversion via an unknown mechanism. The present study investigated the function of ERR in insects using female silkworm (Bombyx mori, Bm). We found that the expression of B. mori vitellogenin (BmVg) and B. mori ERR (BmERR) in the fat bodies of female silkworms at different stages of development exhibited alternating patterns, and RNA interference of BmERR in females induced BmVg transcription, resulting in an increase in egg weight relative to the control. Furthermore, BmERR was found to be involved in regulating the transcription of BmVg through an oestrogen-related receptor response element (ERRE) in the promoter of the BmVg gene, as demonstrated by electrophoretic mobility shift assay, cell transfection assay and chromatin immunoprecipitation. In summary, our results indicate that BmERR bound to the ERRE motif in the BmVg promoter reducing the expression of BmVg in the fat body of the female silkworm. To our surprise, the ERRE also showed the ability to bind the ecdysone receptor (BmEcR) and ultraspiracle complex. Thus, we surmise that ERR participates in steroid hormone signalling by engaging in crosstalk with the ER pathway in vertebrates and with the EcR pathway in insects.

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Vertebrate estrogen regulates the development of female characteristics in silkworm, Bombyx mori

Cited by 12 publications

References 42 publications

Sex differences in the utilization of essential and non-essential amino acids in a Lepidoptera

Sex differences in the utilization of essential and non-essential amino acids in a Lepidoptera

Vertebrate hormones in insects: the role of estrogen in silkworm – a review

Oestrogen‐related receptor reduces vitellogenin expression by crosstalk with the ecdysone receptor pathway in female silkworm, Bombyx mori

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