Versicolorin A enhances the genotoxicity of aflatoxin B1 in human liver cells by inducing the transactivation of the Ah-receptor

Budin, Clémence; Man, Hai-Yen; Al-Ayoubi, Carine; Puel, Sylvie; Vugt-Lussenburg, Barbara M.A. van; Brouwer, Abraham; Oswald, Isabelle P.; Burg, Bart van der; Soler, Laura

doi:10.1016/j.fct.2021.112258

Cited by 14 publications

(5 citation statements)

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“…AFB1 and FB1 individually affected the p53 and complexes, which could result in the MIX significantly upregulated p53 gene and downregulated Cx I, Cx II, Cx III, and Cx IV genes resulting in the significant inhibition interaction on mitochondrial respiration. As reported in the literature, HepG2 cells exposed to either AFB1 or FB1 showed a higher abundance of p53 (Budin et al 2021; Li et al 2021). Moreover, Du et al have shown that when AFB1 and FB1 were combined, a higher optical density of p53 was observed by immunohistochemical analysis, and they hypothesized that there could be an interaction between AFB1 and FB1 in inducing HepG2 cell apoptosis (Du et al 2017).…”

Section: Discussionsupporting

confidence: 81%

Elucidating the combined toxicity of aflatoxin B1 and fumonisin B1 on HepG2 cells based on respirometry and transcriptome analyses

Chen

Abdallah

Grootaert

et al. 2023

Preprint

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Aflatoxin B1 (AFB1) and fumonisin B1 (FB1) are two toxic mycotoxins widely found in food contaminants, and known for their hepatotoxicity in human. However, their combined toxicity still needs to be deeply investigated especially for their harmful effect. Therefore, the current work aimed at investigating the (combined) effect of AFB1 and FB1 on mitochondrial and glycolytic activity of HepG2 cell line, a well-recognized in vitro model system to study liver cell function. In our previous work, we studied the impact of a short term exposure to different doses of AFB1, FB1, and their binary mixture (MIX) on the bioenergetic status of HepG2 cells. Seahorse respirometry analysis revealed that the co-exposure, especially at high doses (8 μg/mL for AFB1 and 160 μg/mL for FB1), is more toxic as a result of more inhibition of all parameters of mitochondrial respiration. RNA transcriptome sequencing showed that the p53 signaling pathway, which is a major orchestrator of mitochondrial apoptosis, was differentially expressed. Moreover, the co-exposure has significantly downregulated Cx I, Cx II, Cx III, and Cx IV genes, which represent the onset of the suppressed mitochondrial respiration in HepG2 cells. It was found that FB1 is contributed more to the MIX effects than AFB1.

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Section: Discussionsupporting

confidence: 81%

Elucidating the combined toxicity of aflatoxin B1 and fumonisin B1 on HepG2 cells based on respirometry and transcriptome analyses

Chen

Abdallah

Grootaert

et al. 2023

Preprint

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“…Recent research reported that Ver A was a food contaminant toxic to human intestinal cells (Gauthier et al, 2020). VerB and versiconol lack the furofuran (bisfuran) C--C double bond structure and are more upstream in aflatoxin biosynthesis, which implied that their toxicity was lower (Budin et al, 2021). In addition, we found that the correlation between VerB and APC was 0.78, which was higher than that between VerA and APC (0.71) (Fig.…”

Section: Discussionmentioning

confidence: 71%

Fungi population metabolomics and molecular network study reveal novel biomarkers for early detection of aflatoxigenic Aspergillus species

Xie

Wang

Hooft

et al. 2022

Journal of Hazardous Materials

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“…Versicolorin A is an intermediate in the aflatoxin biosynthetic pathway described as a potential indicator of aflatoxin contamination (Zhang et al, 2018). It also increases the hepatic genotoxicity of aflatoxin B 1 (Budin et al, 2021) and is cytotoxic and genotoxic for Caco-2 and HCT116 cells (human colon cell lines) (Gauthier et al, 2020). Hepatocarcinogenicity was also observed in rainbow trout embryos one year after exposure to versicolorin A (Hendricks et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin biosynthetic pathway extrolites in airborneAspergilli seriesVersicolores

Géry

Basset

Gounel

et al. 2023

WMJ

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The Aspergilli of the series Versicolores include several airborne species to which we are exposed daily. Most of them synthesise sterigmatocystin, a mycotoxin precursor of aflatoxins recognised as potentially carcinogenic for humans (group 2B) by the International Agency for Research on Cancer. Our objective was to study the potential role of these moulds in the synthesis of extrolites of the aflatoxin biosynthetic pathway. A total of 124 isolates belonging to the eight species of the series Versicolores collected in French bioaerosols were studied: Aspergillus amoenus (n=1), Aspergillus creber (n=45), Aspergillus fructus (n=2), Aspergillus jensenii (n=39), Aspergillus protuberus (n=6), Aspergillus puulaauensis (n=5), Aspergillus sydowii (n=14) and Aspergillus tabacinus (n=2). All these isolates and a reference strain of each species were extracted using ethyl acetate acidified with 1% (v/v) acetic acid. In each extract, we screened for the following extrolites of the aflatoxin biosynthetic pathway by UPLC-HRMS: norsolorinic acid, versicolorin A, 6-demethylsterigmatocystin, sterigmatocystin, 8-O-methylsterigmatocystin, 5-methoxysterigmatocystin and aflatoxins B1, B2, G1 and G2. All extrolites, except aflatoxins, were found variably among species. Sterigmatocystin was found in extracts of all eight Versicolores species, including A. sydowii and A. tabacinus whose ability to synthesise sterigmatocystin was questioned or unknown, respectively.

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Versicolorin A enhances the genotoxicity of aflatoxin B1 in human liver cells by inducing the transactivation of the Ah-receptor

Cited by 14 publications

References 46 publications

Elucidating the combined toxicity of aflatoxin B1 and fumonisin B1 on HepG2 cells based on respirometry and transcriptome analyses

Elucidating the combined toxicity of aflatoxin B1 and fumonisin B1 on HepG2 cells based on respirometry and transcriptome analyses

Fungi population metabolomics and molecular network study reveal novel biomarkers for early detection of aflatoxigenic Aspergillus species

Aflatoxin biosynthetic pathway extrolites in airborneAspergilli seriesVersicolores

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