Versican in epithelial ovarian cancer: Relation to hyaluronan, clinicopathologic factors and prognosis

Voutilainen, Kirsi; Anttila, Markku; Sillanpää, Sari; Tammi, Raija; Tammi, Markku; Saarikoski, Seppo; Kosma, Veli‐Matti

doi:10.1002/ijc.11423

Cited by 98 publications

(103 citation statements)

References 33 publications

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“…Versican expression was normalized against a household gene (EEF1A1). 12,13,[16][17][18]20,23,34,[36][37][38][39] As the composition of the ECM influences the migration of inflammatory cells, 33 we have also measured the association between inflammatory cells and versican expression. Despite the reported production of versican isoforms V0 and V1 by human leukemic monocytes 40 and the reported …”

Section: Discussionmentioning

confidence: 99%

“…[11][12][13][14] Versican expression promotes tumor growth by destabilizing focal cell contacts, thus hampering cell adhesion and regulating angiogenesis. 10,15 Indeed, increased levels of versican have been reported in different tumor types such as adenocarcinomas, [16][17][18] squamous cell carcinomas, 19,20 sarcomas, 21 mesotheliomas 22 and malignant melanomas. 23 In addition, versican expression has been associated with tumor progression and decreased survival in various tumor types.…”

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confidence: 99%

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Versican expression is associated with tumor-infiltrating CD8-positive T cells and infiltration depth in cervical cancer

et al. 2010

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Cervical carcinoma is the second most frequent cancer type in women worldwide. Both inflammatory cells and stromal cells are important for tumor progression. Stromal cells produce growth factors and extracellular matrix and provide an adequate environment for angiogenesis. Versican, a member of the extracellular matrix, has been shown to have a role in tumor progression. The aim of this study was to investigate versican expression, and its association with tumor-infiltrating inflammatory cell subsets and with clinicopathological parameters in human cervical cancers. We have studied the expression of versican in 149 cervical cancers using immunohistochemistry and mRNA in situ hybridization. Versican was predominantly expressed in the stroma (myofibroblasts). Using quantitative real-time-PCR, V0 was found to be the most prominent isoform. High stromal versican expression was significantly associated with a low number of tumor-infiltrating T cells (P ¼ 0.018) and particularly a low number of CD8-positive T cells (cytotoxic T cells; P ¼ 0.002). Stromal versican expression was significantly higher in patients with an infiltration depth 414 mm (P ¼ 0.004) and in patients with parametrial invasion (P ¼ 0.044). Stromal versican expression was not associated with survival. Our results suggest that versican expression in the stromal compartment of cervical cancers results in reduced numbers of intraepithelial CD8-positive T cells and enhanced local invasion.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Versican expression is associated with tumor-infiltrating CD8-positive T cells and infiltration depth in cervical cancer

et al. 2010

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“…Studies on chondroitin sulfate proteoglycans have shown that they play important roles in cancer biology, and may be useful prognostic factors in breast, lung, ovarian and skin cancers. 10,13,14,[29][30][31] Several immunohistochemical studies focusing on the sulfated chondroitin chain have shown that high stromal levels of this molecule is associated with a poor outcome in patients with early-stage prostate cancer. 19,20 Furthermore, increased expression of the core proteins of versican in the peritumoral stroma, and PTEN (two chondroitin sulfate proteoglycans) has also been reported to be predictive of poor prognosis in early-stage prostate cancer patients.…”

Section: Discussionmentioning

confidence: 99%

“…These have been found to regulate cancer cell behavior and affect disease outcome in a number of malignancies, including cancers of the lung, breast, ovary, mouth, pharynx and skin. [10][11][12][13][14][15] Previous studies have shown that expression of versican, a chondroitin sulfate proteoglycan, is increased in prostate cancer and inhibits cancer cell adhesion to fibronectin in the stromal matrix. 16,17 Furthermore, high performance liquid chromatography analysis of glycosaminoglycans extracted from prostate cancer samples revealed that chondroitin-6-sulfate and dermatan sulfate were the predominantly upregulated components.…”

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confidence: 99%

Increased expression of non-sulfated chondroitin correlates with adverse clinicopathological parameters in prostate cancer

et al. 2008

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Chondroitin sulfate is a structurally diverse glycosaminoglycan, which contains a variable degree of sulfation that helps to determine its biological function. It is involved in the regulation of cellular activity and has been implicated in carcinogenesis. To determine if the non-sulfated chondroitin backbone has a functional role in prostate cancer, we analyzed its expression by immunohistochemistry using the 1B5 monoclonal antibody and a set of tissue microarrays constructed with 227 prostate specimen cores from 81 cases of benign prostate tissue and 77 cases of prostate cancer, of which 69 of these cases are matched. Non-sulfated chondroitin was found in the secretory epithelial cells and stromal regions of both prostatic adenocarcinoma and benign prostatic tissues, as well as in the basal cells of benign glands. A higher percentage of cancerous cells were stained positively for non-sulfated chondroitin as compared with benign secretory cells of the same patient. Cancerous cells stained more intensely for non-sulfated chondroitin. This increase in percentage of cells stained and increase in staining intensity were associated with higher pathological T stage and extraprostatic extension. Non-sulfated chondroitin expression (either staining intensity or percentage of cells stained) in adenocarcinoma and its peritumoral stroma correlated significantly with several clinicopathological parameters of unfavorable outcome, including higher pathological T stage and Gleason score, presence of tumor in both prostatic lobes, extraprostatic extension, seminal vesicle involvement and preoperative prostate-specific antigen levels. These data suggest that non-sulfated chondroitin is a potentially useful biomarker for prostate cancer, and may be involved in regulating prostate cancer behavior.

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“…Versican belongs to the family of large chondroitin sulfate proteoglycans located within the ecM (2,3). its overproduction is a common feature of several tumor types, and is usually related to a poor prognosis, for example in the case of prostate (4), breast (5), ovarian (6), lung (7) and oral (8) cancer, and melanomas (9,10).…”

Section: Introductionmentioning

confidence: 99%

Differential expression of endoglin in human melanoma cells expressing the V3 isoform of versican by microarray analysis

et al. 2010

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Abstract. Versican is a large chondroitin sulfate proteoglycan produced by several tumor types, including malignant melanoma, which exists as four different splice variants. The large isoforms V0 and V1 promote melanoma cell proliferation. We previously described that overexpression of the short V3 isoform in MeWo human melanoma cells markedly reduced tumor cell growth in vitro and in vivo, but favored the appearance of secondary tumors. This study aimed to elucidate the mechanisms of V3 by identifying differentially expressed genes between parental and V3-expressing MeWo melanoma cells using microarray analysis. V3 expression significantly reduced the expression of endoglin, a transforming growth factor-β superfamily co-receptor. other differentially expressed genes were VEGF and PPP1R14B. changes in endoglin levels were validated by qrT-Pcr and Western blotting.

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Versican in epithelial ovarian cancer: Relation to hyaluronan, clinicopathologic factors and prognosis

Cited by 98 publications

References 33 publications

Versican expression is associated with tumor-infiltrating CD8-positive T cells and infiltration depth in cervical cancer

Versican expression is associated with tumor-infiltrating CD8-positive T cells and infiltration depth in cervical cancer

Increased expression of non-sulfated chondroitin correlates with adverse clinicopathological parameters in prostate cancer

Differential expression of endoglin in human melanoma cells expressing the V3 isoform of versican by microarray analysis

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