Versatility of choline metabolism and choline-binding proteins in<i>Streptococcus pneumoniae</i>and commensal streptococci

Hakenbeck, Regine; Madhour, Abderrahim; Denapaite, Dalia; Brückner, Reinhold

doi:10.1111/j.1574-6976.2009.00172.x

Cited by 70 publications

(91 citation statements)

References 143 publications

(196 reference statements)

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“…Genes for methylenetetrahydrofolate reductase, which is involved in methionine production, mapped principally to S. sanguinis and while the gene has been detected in bacteria no specific role in the oral biofilm community was found. Choline kinase genes mapped mainly to S. mitis as previously reported [42] and are considered to play a role in cell wall lysis. It is not clear what their role in caries-free sites would be.…”

Section: Discussionmentioning

confidence: 74%

Functional profiles of coronal and dentin caries in children

Kressirer

Chen

Harriman³

et al. 2018

Journal of Oral Microbiology

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Background: Dental caries results from a dysbiosis of tooth-associated biofilms and frequently extends through enamel into dentin which has a different structure and composition.Objective: To evaluate the metatranscriptome of caries to determine the metabolic potential of caries communities compared with health.Design: Samples from children, caries-free (CF: n = 4) or with coronal (CC: n = 5) or dentin (DC: n = 5) caries were examined for gene expression potential. Functional profiling was performed using HUMAnN2 (HMP Unified Metabolic Analysis Network).Results: There was increased gene expression diversity in DC compared with CC and CF. Genes in CF included alcohol dehydrogenase from Neisseria sicca, methylenetetrahydrofolate reductase from Streptococcus sanguinis and choline kinase from streptococci. Genes in CC mapped mainly to Streptococcus mutans. Arginine deiminase in DC mapped to S. sanguinis and Actinomyces naeslundii. Glycerol kinase genes mapped to S. sanguinis in all groups whereas glycerol kinase in DC were from Rothia, Prevotella and streptococci. Uracil-DNA glycosylase in DC mapped to Prevotella denticola and Actinomyces. Repressor LexA in DC mapped to Scardovia wiggsiae, Dialister invisus and Veillonella parvula.Conclusions: Functional profiling revealed enzyme activities in both caries and caries-free communities and clarified marked differences between coronal and dentin caries in bacterial composition and potential gene expression.

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Section: Discussionmentioning

confidence: 74%

Functional profiles of coronal and dentin caries in children

Kressirer

Chen

Harriman³

et al. 2018

Journal of Oral Microbiology

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“…A family of bacterial proteins, broadly called choline-binding proteins (CBPs), use choline binding as a strategy to get noncovalently anchored to the cholinecontaining gram-positive cell wall after being exported to the bacterial surface. Each choline-binding module consists of multiple tandem repeats of choline-binding motif made of 20-amino acid residues with a consensus sequence of W-6X-WY (21). Sequence comparison and alignment of PsaA with choline-binding repeats of CBP of pneumococci ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Structural basis for the specific recognition of dual receptors by the homopolymeric pH 6 antigen (Psa) fimbriae of Yersinia pestis

Bao

Nair

Tang

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The pH 6 antigen (Psa) of Yersinia pestis consists of fimbriae that bind to two receptors: β1-linked galactosyl residues in glycosphingolipids and the phosphocholine group in phospholipids. Despite the ubiquitous presence of either moiety on the surface of many mammalian cells, Y. pestis appears to prefer interacting with certain types of human cells, such as macrophages and alveolar epithelial cells of the lung. The molecular mechanism of this apparent selectivity is not clear. Site-directed mutagenesis of the consensus choline-binding motif in the sequence of PsaA, the subunit of the Psa fimbrial homopolymer, identified residues that abolish galactosylceramide binding, phosphatidylcholine binding, or both. The crystal structure of PsaA in complex with both galactose and phosphocholine reveals separate receptor binding sites that share a common structural motif, thus suggesting a potential interaction between the two sites. Mutagenesis of this shared structural motif identified Tyr126, which is part of the choline-binding consensus sequence but is found in direct contact with the galactose in the structure of PsaA, important for both receptor binding. Thus, this structure depicts a fimbrial subunit that forms a polymeric adhesin with a unique arrangement of dual receptor binding sites. These findings move the field forward by providing insights into unique types of multiple receptor-ligand interactions and should steer research into the synthesis of dual receptor inhibitor molecules to slow down the rapid progression of plague.adhesin-receptor complex | adhesion | ligand binding | sugar binding | phosphocholine binding

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“…However, proteins found on the surface of pneumococci and other Gram-positive organisms can lack standard signal sequences (9,10). The genome of the R6 strain does not have CbpI and CbpJ (8). Some of the CBPs, including PspA, PspC, and PcpA, have been described to be important virulence factors (7,(11)(12)(13), and PspA has been shown to be the major protein among the CBPs (14,15).…”

mentioning

confidence: 99%

“…The TIGR4 genome also contains two open reading frames that have the choline-binding domains, but the proteins are truncated or degenerated (8). Some of the CBPs do not have signal secretion sequences.…”

mentioning

confidence: 99%

“…CBPs have a biologically active module and a choline-binding module that anchors these proteins noncovalently to the phosphorylcholine of teichoic and lipoteichoic acids. The number of CBPs varies in different strains (but is approximately 15), and some of these proteins are highly variable (5,8). The genome of the TIGR4 strain has genes encoding the CBPs CbpI (choline-binding protein I), PspA (pneumococcal surface protein A), CbpC (choline-binding protein C), CbpJ (cholinebinding protein J), CbpG (choline-binding protein G), CbpF (choline-binding protein F), Pce (phosphorylcholine esterase), LytB (autolysin B), LytC (autolysin C), LytA (autolysin A), PcpA (pneumococcal choline-binding protein A), PspC (pneumococcal surface protein C), and CbpD (choline-binding protein D).…”

mentioning

confidence: 99%

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Evaluation of a Vaccine Formulation against Streptococcus pneumoniae Based on Choline-Binding Proteins

Miyaji

Vadesilho

Oliveira

et al. 2014

Clin. Vaccine Immunol.

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dStreptococcus pneumoniae has proteins that are attached to its surface by binding to phosphorylcholine of teichoic and lipoteichoic acids. These proteins are known as choline-binding proteins (CBPs). CBPs are an interesting alternative for the development of a cost-effective vaccine, and PspA (pneumococcal surface protein A) is believed to be the most important protective component among the different CBPs. We sought to use CBPs eluted from pneumococci as an experimental vaccine. Since PspA shows variability between isolates, we constructed strains producing different PspAs. We used the nonencapsulated Rx1 strain, which produces PspA from clade 2 (PspA2), to generate a pspA-knockout strain (Rx1 ⌬pspA) and strains expressing PspA from clade 1 (Rx1 pspA1) and clade 4 (Rx1 pspA4). We grew Rx1, Rx1 ⌬pspA, Rx1 pspA1, and Rx1 pspA4 in Todd-Hewitt medium containing 0.5% yeast extract and washed cells in 2% choline chloride (CC). SDS-PAGE analysis of the proteins recovered by a CC wash showed few bands, and the CBPs PspA and PspC (pneumococcal surface protein C) were identified by mass spectrometry analysis. Subcutaneous immunization of mice with these full-length native proteins without adjuvant led to significantly higher rates of survival than immunization with diluent after an intranasal lethal challenge with two pneumococcal strains and also after a colonization challenge with one strain. Importantly, immunization with recombinant PspA4 (rPspA4) without adjuvant did not elicit significant protection.

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Versatility of choline metabolism and choline-binding proteins inStreptococcus pneumoniaeand commensal streptococci

Cited by 70 publications

References 143 publications

Functional profiles of coronal and dentin caries in children

Functional profiles of coronal and dentin caries in children

Structural basis for the specific recognition of dual receptors by the homopolymeric pH 6 antigen (Psa) fimbriae of Yersinia pestis

Evaluation of a Vaccine Formulation against Streptococcus pneumoniae Based on Choline-Binding Proteins

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