Versatility in lipid compositions showing prolonged circulation with sterically stabilized liposomes

Woodle, Martin C.; Matthay, Katherine K.; Newman, Mary S.; Hidayat, Jon; Collins, Lila R.; Redemann, Carl T.; Martin, Frank; Papahadjopoulos, Demetrios

doi:10.1016/0005-2736(92)90194-q

Cited by 227 publications

(112 citation statements)

References 16 publications

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“…The blood clearance curve of 111 In encapsulated in sterically stabilized pH-sensitive liposomes (Fig. 3A) was similar to that of previously developed liposomes with prolonged circulation time [54,55]. A substantial percentage of liposomes (8.5%) remained in the blood after 24 h. In contrast, the radioactive marker encapsulated in regular pH-sensitive liposomes was almost completely eliminated from the bloodstream within 0.5 h. The t 1/2 of control DSPC/CHEMS/PE -PEG liposomes and sterically stabilized pH-sensitive DOPE/CHEMS/PE -PEG liposomes was similar (11.8 F 0.7 and 11.1 F 0.6 h, respectively).…”

Section: Long-circulating Dope-containing Ph-sensitive Liposomessupporting

confidence: 82%

On the formulation of pH-sensitive liposomes with long circulation times

Simões

Moreira

Fonseca

et al. 2004

Advanced Drug Delivery Reviews

435

244

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Strategies used to enhance liposome-mediated drug delivery in vivo include the enhancement of stability and circulation time in the bloodstream, targeting to specific tissues or cells, and facilitation of intracytoplasmic delivery. pH-sensitive liposomes have been developed to mediate the introduction of highly hydrophilic molecules or macromolecules into the cytoplasm. These liposomes destabilize under acidic conditions found in the endocytotic pathway, and usually contain phosphatidylethanolamine (PE) and titratable stabilizing amphiphiles. Formulations without PE have also been developed. Encapsulated compounds are thought to be transported into the cytoplasm through destabilization of or fusion with the endosome membrane. Incorporation of a low mole percentage of poly(ethylene glycol) (PEG)-conjugated lipids into pHsensitive liposomes confers prolonged circulation times to these liposomes, which are otherwise cleared rapidly. While the incorporation of PEG -lipids reduces the pH-dependent release of encapsulated fluorescent markers in vitro, it does not hinder the cytoplasmic delivery of the markers per cell-associated liposome. This suggests that intracellular delivery is not dictated simply by the destabilization of the liposomes. Antibodies or ligands to cell surface receptors can be coupled to pH-sensitive or sterically stabilized pH-sensitive liposomes for targeting. pH-sensitive liposomes have been used to deliver anticancer drugs, antibiotics, antisense oligonucleotides, ribozymes, plasmids, proteins and peptides to cells in culture or in vivo. D

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Section: Long-circulating Dope-containing Ph-sensitive Liposomessupporting

confidence: 82%

On the formulation of pH-sensitive liposomes with long circulation times

Simões

Moreira

Fonseca

et al. 2004

Advanced Drug Delivery Reviews

435

244

View full text Add to dashboard Cite

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“…following intravenous administration were nerformed with male and female adult Sprague-Dawley rats (22&400 g) following standard procedures described elsewhere [20]. Briefly, liposome samples prepared at 10 mM phospholipid in 10 mM desferoxamine mesylate in isotonic saline and radiolabeled with 67Ga-oxine 1151 were administered intravenously at a dose of about 10-20 mm01 -phospholipid/kg body weight.…”

Section: Methodsmentioning

confidence: 99%

“…Liposomes composed of amino-PEG-DSPE, partially hydrogenated egg PC, and cholesterol in a mole ratio of 0.15 : 1.85 : 1 with an average particle size distribution of 1000 A and 30 to 100 mmol phospholipid/ml were prepared by extrusion of multilamellar vesicles (MLV) using defined pore filters (Nuclepore) according to Woodle et al [20]. Loading of 67Ga-DF as a liposomal radiolabel was achieved as described previously [15,20].…”

Section: Preparation Of Liposomes and In Vivo Studiesmentioning

confidence: 99%

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Long circulating, cationic liposomes containing amino‐PEG‐phosphatidylethanolamine

Zalipsky¹,

Brandeis²,

Newman³

et al. 1994

FEBS Letters

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Ah&actLigand attachment to polyethylene glycol (PEG) grafted, long circulating liposomes at the polymer terminus is of interest for targeting but the effect of positively charged groups is unknown. Amino-polyethylene glycol-phosphatidylethanolamine (AminoPEG-PE), prepared in four steps from a-amino-o+hydroxy-PEG, was tested for intluence on liposome interactions in vivo: blood circulation and biodistribution. Despite surface amines on each liposome conferring cationic behavior, in vivo properties are comparable to those obtained with methoxy-PEG-PE. The consequences are profound for targeting and possibly systemic delivery of cationic lipidic-polynucleotide complexes.

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“…To date, many reports have described the use of PEGylated liposomes for long blood circulation. Woodle et al reported the effect of various molecular weights (120, 750, 1,900, and 5,000) of PEG conjugated with distearoylphosphatidylethanolamine (DSPE)-modified liposomes on prolonged blood circulation [114]. They reported that longer forms of PEG with molecular weights of 1,900 and 5,000 kDa used to PEGylate liposomes with a size of about 100 nm prolonged their circulation in the blood.…”

Section: Overcoming the Rapid Clearance Of Liposomesmentioning

confidence: 99%

Glycosylation-mediated targeting of carriers

Kawakami

Hashida

2014

Journal of Controlled Release

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For safe and effective therapy, drugs should be delivered selectively to their target tissues or cells at an optimal rate. Drug delivery system technology maximizes the therapeutic efficacy and minimizes unfavorable drug actions by controlling their distribution profiles. Ligand-receptor binding is a typical example of specific recognition mechanisms in the body; therefore, ligand-modified drug carriers have been developed for active targeting based on receptor-mediated endocytosis. Among the various ligands reported thus far, sugar recognition is a promising approach for active targeting because of their high affinity and expression. Glycosylation has been applied for both macromolecular and liposomal carriers for cell-selective drug targeting.Recently, the combination of ultrasound exposure and glycosylated bubble liposomes has been developed. In this review, recent advances of glycosylation-mediated targeted drug delivery systems are discussed.

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Versatility in lipid compositions showing prolonged circulation with sterically stabilized liposomes

Cited by 227 publications

References 16 publications

On the formulation of pH-sensitive liposomes with long circulation times

On the formulation of pH-sensitive liposomes with long circulation times

Long circulating, cationic liposomes containing amino‐PEG‐phosphatidylethanolamine

Glycosylation-mediated targeting of carriers

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