Versatile Laboratory Concentration Device

Craig, Lyman C.; Gregory, J D.; Hausmann, W.

doi:10.1021/ac60047a601

Cited by 128 publications

(25 citation statements)

References 1 publication

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“…The total derived from the column may be expected to be low for several reasons. In the first place, proline, which probably contributes 10 to 20 mg. of alpha-amino nitrogen, is not listed in 8 per cent cross-linking), 0.9 X 100 cm., was operated in the sodium form, with buffers of the pH and temperature indicated as eluants.…”

Section: Resultsmentioning

confidence: 99%

“…of water, void again after 1 to 2 hours and reject the specimen, drink an additional glass or two of water, and collect the urine during the ensuing 1 to 4-hour interval, the duration of which was recorded. The volume and specific gravity of the specimen were measured, and the urine concentrated in vacuo in a rotary evaporator (8) to a sufficiently small volume to raise the specific gravity to about 1.02 to 1.03. Usually, 2 cc. aliquots of samples of urine from patients with Wilson's disease (3 cc.…”

Section: Methodsmentioning

confidence: 99%

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The Amino Acid Content of the Blood and Urine in Wilson's Disease

Stein¹,

Bearn²,

Moore³

1954

J. Clin. Invest.

118

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Since the report of Uzman and Denny-Brown (1) that Wilson's disease was associated with an aminoaciduria, several studies of this aspect of the disease have appeared. Dent and Harris (2) employed paper chromatograms to demonstrate the generalized nature of the aminoaciduria, while Cooper, Eckhardt, Faloon, and Davidson (3) employed microbiological assay to determine quantitatively the extent of the excretion of eight essential and two non-essential amino acids. Investigations of the blood plasma by these investigators (3) and by Matthews, Milne, and Bell (4), who determined total a-amino nitrogen, have not revealed any abnormalities in the concentration of amino acids in plasma, however. With the development of chromatographic methods employing columns of ion exchange resin, it has become possible to determine quantitatively and simultaneously the concentration of all of the ninhydrinpositive substances in normal and pathological urines (5) and in plasma (6). The application of these methods to a detailed study of the amino acid distribution in the urine and plasma of patients suffering from Wilson's disease forms the subject of this communication.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The Amino Acid Content of the Blood and Urine in Wilson's Disease

Stein¹,

Bearn²,

Moore³

1954

J. Clin. Invest.

118

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“…Plasma was separated by centrifugation, pooled, and the total lipids extracted with 2: 1 chloroform-methanol mixture (v/v) and purified according to the method of Folch and co-workers (32). The solvents were evaporated at 450 C. in vacuo 1153 in a flash evaporator (33). The total lipids were dissolved in a chloroform-methanol mixture.…”

Section: Methodsmentioning

confidence: 99%

The Fatty Acids of Total Lipids and Cholesterol Esters from Normal Plasma and Atheromatous Plaques1

Tuna¹,

Reckers²,

Frantz³

1958

J. Clin. Invest.

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In order to elucidate the mechanism of atheroma formation in arteriosclerosis, the lipid fractions of plasma and atheromatous plaques have been extensively studied in the past 45 years. It was first noted that there is a high content of cholesterol, phospholipid and total extractable lipids in atheromatous aortas (1-4). Then came a period when studies dealt primarily with the changing ratios between total, free and esterified cholesterol and phospholipids in normal plasma and atheromatous plaques (5-8). Considerable work has been done in the past decade to correlate the changes in blood cholesterol, phospholipids and lipoproteins with the incidence of arteriosclerosis (9-15). Comparatively little work has been done, however, on the isolation and identification of fatty acids associated with different lipid fractions of plasma and atheromatous plaques, the lack of adequate methods for fatty acid analysis being the most likely explanation. Schoenheimer (3) isolated the cholesterol esters of stearic, oleic and palmitic acids from the adventitia-free atheromatous aorta. In addition to these acids, McArthur (16) isolated linoleic and arachidonic acids from aortic plaques. As far as the fatty acids of the blood are concerned, the main sources of information are alkali isomerization studies (17-19) and studies done on the blood of large mammals (20). Recently, more information based on paper (21), column (22) and gas-liquid (23) chromatographic analysis of fatty acids of human blood has been reported. However, no comparative studies are available on material from human beings, using the same analytical methods to determine the fatty acids in blood and atheromatous plaques. There have been isolated reports suggesting that the blood of popu-

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“…sample of the culture supernatant fluid was concentrated by evaporation at 37" under reduced pressure to about 50 ml. in the apparatus of Craig (1950) and then further concentrated to 2.0 ml. by dialysis, under pressure, against M-phosphate (pH 7.0).…”

Section: Applicationsmentioning

confidence: 99%

An Immunological Study of the Constitutive and the Penicillin-induced Penicillinases of Bacillus cereus, Based on Specific Enzyme Neutralization by Antibody

Pollock

1956

Journal of General Microbiology

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SUMMARY: Exocellular penicillinases from strains 569 and 5/B of BuciZZus cereus gave almost identical activity-neutralization curves when titrated with antisera prepared against the enzyme from either strain ; but were easily distinguishable by differences in their neutralization reaction with an anti-569 penicillinase serum previously partially absorbed with either the 5/B or the 569 enzyme. By contrast, no differences in neutralization reaction were detected between basal and induced penicillinase from strain 569 and the constitutive penicillinase from strain 569/H (a mutant derived from strain 569), when treated with samples of the anti-569 penicillinase serum, previously partially absorbed with each penicillinase preparation separately.Strain 569 was not found to produce any substance in significant quantities capable of combining with anti-penicillinase neutralizing antibodies other than penicillinase itself.

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Versatile Laboratory Concentration Device

Cited by 128 publications

References 1 publication

The Amino Acid Content of the Blood and Urine in Wilson's Disease

The Amino Acid Content of the Blood and Urine in Wilson's Disease

The Fatty Acids of Total Lipids and Cholesterol Esters from Normal Plasma and Atheromatous Plaques1

An Immunological Study of the Constitutive and the Penicillin-induced Penicillinases of Bacillus cereus, Based on Specific Enzyme Neutralization by Antibody

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