The Environmental Mapping and Analysis Program HyperSpectral Imager (EnMAP HSI) will allow to acquire images of the Earth surface in a push-broom configuration. Spectral coverage includes 230 wavelength bands between 420 nm and 2450 nm, which are simultaneously recorded with a ground resolution of 30 m x 30 m.High requirements on the optical alignment of the Visual Near InfraRed (VNIR) and Short Wavelength InfraRed (SWIR) spectrometers and cameras have been fulfilled (one pixel lateral alignment and below 20 μm for defocus). In addition, a calibration and characterization campaign of the EnMAP HSI was performed, with the aim to verify the instrument requirements and to quantify its performance. The campaign addressed the following five responsivity domains: radiometric, polarization, spectral responsivity, spatial responsivity and stray light. A spectral calibration accuracy of 0.5 nm for VNIR and 1 nm for SWIR channel, and lateral calibration of the full field-of-view with sub-arc second accuracy was achieved. For all measurements, traceability to national standards is guaranteed by comparative measurements with calibrated devices.The paper presents the setups of the alignment, calibration and characterization measurements of the EnMAP HSI. Instrument requirements on calibration accuracy are reported. The traceability to national standards will be described and the validation of measurement accuracy.