Verrucosispora sp. K2-04, Potential Xylanase Producer from Kuantan Mangrove Forest Sediment

Abstract

Xylanase is the key enzyme that involves in the hydrolysis of xylan, the main constituent of the complex hemicellulose of the plant cell wall. In this study, forty actinomycetes that were isolated from the sediment of Kuantan Mangrove Forest, Malaysia, were tested for their ability to produce extracellular xylanase. At least 15 isolates were able to degrade xylan in the primary agar-based screening on marine agar containing 0.1% (v/v) azo-xylan (Birchwood). The degradation of xylan was indicated by the formation of halo zone around the colonies and the clear zone index (CZI) was calculated as a ratio of the clearing zones to the colony size. Isolate K2-04 with CZI 3.35 ± 1.91 was identified through 16S rRNA study as Verrucosispora sp. This isolate was further grown in marine broth and incubated at 30 °C, 200 rpm for 20 days. The growth of K2-04 and the xylanase activity was measured at day 2, 4, 6,12 and 18 respectively. The highest enzyme activity of the crude enzyme was recorded at day 18 (1.836 U/mL) and exhibited stability after 20 days storage at 4°C. This study serves as a preliminary study to characterize the properties of Verrucosispora sp. K2-04, rare actinomycete of Kuantan Mangrove Forest, Malaysia.  Index Terms-marine Actinomycetes, xylanase, mangrove, Verrucosispora containing 1 g of dried sediment and 9 ml of sterilized sea water was heated in a water bath at 60°C for 20 min. Morphological differences among the isolates were

Efeito Da Antropização Sobre a Produção Da Enzima Xilanase Em Actinobatérias

“…Esses índices estão de acordo com o encontrado na literatura, Omar et al (2017), isolaram cepas de um manguezal, na Malásia, que apresentaram um índice enzimático máximo de 3,35, valor próximo dos encontrados nesse trabalho, assim como Sipriyadi et al (2016) que obtiveram 8 cepas, das 35 isoladas do solo de uma floresta, na Indonésia, que produziram xilanase e um IE máximo de 3,25. Sanjivkumar et al (2017), avaliaram 40 cepas oriundas de um manguezal na Índia, das quais apenas 4 cepas eram capazes de degradar a xilana, as quais apresentaram uma zona hidrolítica máxima de 29 mm de diâmetro, enquanto que o halo máximo encontrado nesse trabalho foi de 39 mm de diâmetro.…”

Invited Review: Biodiversity of Plant Polysaccharide-Degrading Bacteria in Mangrove Ecosystem

“…A highest extracellular xylanse producing strain, K2-04, was identified as Verrucosispora sp. through 16S rRNA analysis (Omar et al 2017). V. wenchangensis isolated from mangrove soil of Wenchang, Hainan, China, also degraded xylan (Xie et al 2012).…”

“…A Streptomyces sp., which is able to produce a highly alkaline and thermotolerant xylanase, was isolated from mangrove forest of Kadalundi, Kerala, India (Thomas et al 2013). Fifteen xylan degrading bacteria were isolated from mangrove sediment of Kuantan, Malaysia by Omar and co-researchers (Omar et al 2017). A highest extracellular xylanse producing strain, K2-04, was identified as Verrucosispora sp.…”

Xylanases from marine microorganisms: A brief overview on scope, sources, features and potential applications

“…Mangroves are salt-tolerant forest ecosystems that are situated between terrestrial and marine environments of tropical and subtropical latitudes [55]. These ecosystems can thus be found in blackwater, creeks, delta regions, intertidal zones of estuaries, islands, lagoons, marshes and mudflats, and salt pans [39]. The geochemical features of mangroves, such as nutrient concentration, salinity and soil humidity are modified regularly according to the cyclic tidal flooding, making these dynamic environments unique [56].…”

“…Marine microorganisms that thrive in such hostile ecosystems are potential sources of resilient enzymes covering an array of catalytic activities that are appealing for industrial applications, such as the production of biofuels, detergents, food, feed, pharmaceuticals, and leather [13,28,[31][32][33][34][35]. The marine environment is also a source of xylan, and, accordingly, of xylanases [36][37][38][39][40][41]. Tapping the marine environment for microorganisms with xylanase activity is still surpassed by screening efforts of terrestrial sources.…”