Vero cells infected with the Lederle strain of canine distemper virus have increased Fas receptor signaling expression at 15 h post-infection

Puerto, Helen L. Del; Martins, Alberto Mesaque; Braz, Gissandra Farias; Alves, Fabiana; Heinemann, Marcos Bryan; Rajão, Daniela S.; Araujo, Fabiano Conde; Martins, Silva; Nascimento, Deborah Ribeiro; Leite, Rômulo Cerqueira; Vasconcelos, A.C.

doi:10.4238/2011.october.18.3

Cited by 4 publications

(7 citation statements)

References 15 publications

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“…Similar result was observed in CDV infected acute B cell lymphoma cells when 50% of infected cells display apoptosis at 48 h p. i . According to previous studies, CDV infection activates both intrinsic and extrinsic apoptosis pathways, similarly to what has been observed here, almost always leading infected cells to death Moreover, a relationship between viral infection and apoptosis has been reported in HeLa cells by intrinsic pathway through activation of caspase‐3 at 24 h p. i . Cancer is a complex, multi‐step gene‐derived disease with astonishingly numerous links cell death …”

Section: Discussionsupporting

confidence: 88%

“…6,10 In addition, CDV has been shown to induce apoptosis of Vero cells by triggering the extrinsic pathway, with caspase-8 and caspase -3 activation. 11,12 The cellular stress caused by CDV infection of HeLa cells leading to apoptosis may represent an attractive alternative for cancer treatment. 11 TNFAIP8 like-2 (TIPE2), a member of the tumour necrosis factor-𝛼-induced protein-8 (TNFAIP8) family, was recently identified as a novel negative regulator gene of the immune system which can independently maintain immune homeostasis.…”

Section: Introductionmentioning

confidence: 99%

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Expression of Concern: Tumour necrosis factor‐alpha‐induced protein 8 (TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus

Garcia

Ferreira²,

Vieira

et al. 2015

Vet Comparative Oncology

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Oncolytic virotherapy is a novel strategy for treatment of cancer in humans and companion animals as well. Canine distemper virus (CDV), a paramyxovirus, has proven to be oncolytic through induction of apoptosis in canine-derived tumour cells, yet the mechanism behind this inhibitory action is poorly understood. In this study, three human mammary tumour cell lines and one canine-derived adenofibrosarcoma cell line were tested regarding to their susceptibility to CDV infection, cell proliferation, apoptosis, mitochondrial membrane potential and expression of tumour necrosis factor-alpha-induced protein 8 (TNFAIP8). CDV replication-induced cytopathic effect, decrease of cell proliferation rates, and >45% of infected cells were considered death and/or under late apoptosis/necrosis. TNFAIP8 and CDVM gene expression were positively correlated in all cell lines. In addition, mitochondrial membrane depolarization was associated with increase in virus titres (p < 0.005). Thus, these results strongly suggest that both human and canine mammary tumour cells are potential candidates for studies concerning CDV-induced cancer therapy.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Expression of Concern: Tumour necrosis factor‐alpha‐induced protein 8 (TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus

Garcia

Ferreira²,

Vieira

et al. 2015

Vet Comparative Oncology

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“…CTSK and CTSV are associated with a mitochondria-dependent intrinsic pathway to trigger the apoptosis of host cells, while TNF-a participates in an extrinsic receptor-mediated pathway (Benedict et al, 2002 ). This finding was consistent with previous reports showing that CDV induces apoptosis in the cerebellum and lymphoid tissues of the natural infection of dogs and in Vero cells in vitro (Moro et al, 2003 ; Del Puerto et al, 2010 , 2011 ). The mechanisms of apoptosis in the pathogenesis of CDV have not yet been clearly illuminated, and the extensive study of these proteins should enhance the current understanding of the mechanisms underlying apoptosis regulation during CDV infection.…”

Section: Discussionsupporting

confidence: 93%

Quantitative Analysis of Cellular Proteome Alterations in CDV-Infected Mink Lung Epithelial Cells

Tong

Sun

et al. 2017

Front. Microbiol.

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Canine distemper virus (CDV), a paramyxovirus, causes a severe highly contagious lethal disease in carnivores, such as mink. Mink lung epithelial cells (Mv.1.Lu cells) are sensitive to CDV infection and are homologous to the natural host system of mink. The current study analyzed the response of Mv.1.Lu cells to CDV infection by iTRAQ combined with LC–MS/MS. In total, 151 and 369 differentially expressed proteins (DEPs) were markedly up-regulated or down-regulated, respectively. Thirteen DEPs were validated via real-time RT-PCR or western blot analysis. Network and KEGG pathway analyses revealed several regulated proteins associated with the NF-κB signaling pathway. Further validation was performed by western blot analysis and immunofluorescence assay, which demonstrated that different CDV strains induced NF-κB P65 phosphorylation and nuclear translocation. Moreover, the results provided interesting information that some identified DEPs possibly associated with the pathogenesis and the immune response upon CDV infection. This study is the first overview of the responses to CDV infection in Mv.1.Lu cells, and the findings will help to analyze further aspects of the molecular mechanisms involved in viral pathogenesis and the immune responses upon CDV infection.

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“…According to the previous study, CDV has been shown to induce apoptosis in vivo, such as in the cerebellum [ 21 ] and lymphoid tissue of naturally infected dogs [ 22 , 23 ]. CDV also causes apoptosis in vitro, such as in Vero [ 24 , 25 ] and HeLa cells [ 26 ]. However, apoptosis in the brain of dogs naturally infected with CDV remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Apoptotic investigation of brain tissue cells in dogs naturally infected by canine distemper virus

Pan

Wang

et al. 2021

Virol J

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Background Canine distemper caused by canine distemper virus that belongs to the Morbillivirus genus of the Paramyxoviridae family is still a global epidemic significant infectious disease, especially in pet dogs in China and serious harm to the development of the dog industry. It has been known that apoptosis caused by the canine distemper virus can show in culture cells, lymphoid tissues, and the cerebellum. However, its occurrence in brain tissue cells remains unclear. To investigate the relationship among canine distemper infecting brain tissues, apoptosis in brain tissue cells, and demyelinating pathogenesis was investigated. Methods 16 naturally infected dogs that exhibited clinical signs of CD and tested positive for the anti-CDV monoclonal antibody and six healthy dogs that served as the control, were used in the research. Brain specimens were divided into the cerebrum, brain stem, and cerebellum embedded in paraffin and made the sections respectively. Approximately 5 µm-thick sections were stained by hematoxylin–eosin, methyl green pyronin, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling technique, and immunohistochemistry. CDV nucleocapsid protein was detected by immune streptavidin-biotinylated peroxidase complex. Results Alterations in the brain tissues of CDV-infected dogs involved both various cells and nerve fibers. CDV had varying degrees of cytotropism to all brain tissue cells; apoptosis also occurred in all brain cells, especially in the endothelia of cerebral vessels, astrocytes, oligodendrocytes, and ependymal cells, the more serious infection, the more obvious apoptosis. Serious infections also involved the pyramidal and Purkinje cells. The nervous fibers exhibited demyelinating lesions (showed small multifocal vacuole), and some axonal neuron atrophy gradually disappeared (formed large vacuole). Conclusions Apoptosis in brain tissue cells was mainly related to the propagation path and cytotropism of CDV. The apoptosis of astrocytes, oligodendrocytes, and some neurons may play a significant role in the demyelinating pathogenesis in dogs with acute canine distemper. A lot of diverse nervous signs shown in the clinic may be related to different neuron apoptosis.

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Vero cells infected with the Lederle strain of canine distemper virus have increased Fas receptor signaling expression at 15 h post-infection

Cited by 4 publications

References 15 publications

Expression of Concern: Tumour necrosis factor‐alpha‐induced protein 8 (TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus

Expression of Concern: Tumour necrosis factor‐alpha‐induced protein 8 (TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus

Quantitative Analysis of Cellular Proteome Alterations in CDV-Infected Mink Lung Epithelial Cells

Apoptotic investigation of brain tissue cells in dogs naturally infected by canine distemper virus

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