Vernalization-Mediated <i>VIN3</i> Induction Overcomes the LIKE-HETEROCHROMATIN PROTEIN1/POLYCOMB REPRESSION COMPLEX2-Mediated Epigenetic Repression

Kim, Dong Hwan; Zografos, Brett R.; Sung, Sibum

doi:10.1104/pp.110.161083

Cited by 42 publications

(44 citation statements)

References 51 publications

(54 reference statements)

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“…The constitutive association of LHP1 with these targets is reminiscent of the data showing that LHP1 remains to be associated with VIN3 chromatin many days after induction by vernalization [25], indicating that the LHP1 binding in that conditions does not lead to gene silencing. These observations collectively suggest that additional elements associated with the H3K27me3/LHP1 module, which can be inactivated by inductive signals, might be involved in H3K27me3/LHP1–mediated gene silencing.…”

Section: Discussionmentioning

confidence: 74%

The Role of Histone Methylation and H2A.Z Occupancy during Rapid Activation of Ethylene Responsive Genes

Shen

Silva

et al. 2011

PLoS ONE

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Ethylene signaling pathway leads to rapid gene activation by two hierarchies of transcription factors with EIN3/EIL proteins as primary ones and ERF proteins as secondary ones. The role of chromatin modifications during the rapid gene activation is not known. In this work we studied trimethylated histone H3 lysine 4 (H3K4me3) and lysine 27 (H3K27me3), two opposite histone methylation marks for gene activity, during the induction course of three ethylene-responsive genes (ERF1, AtERF14 and ChiB). We found that the three genes displayed different histone modification profiles before induction. After induction, H3K4me3 was increased in the 5′ region and the gene body of ERF1, while H3K27me3 was decreased in the promoter of AtERF14. But the modification changes were later than the gene activation. Analysis of other rapidly inducible ERF genes confirmed the observation. In addition, histone H2A.Z occupancy on the three genes and the association of the H3K27me3-binding protein LHP1 with AtERF14 and ChiB were not affected by the inductive signal. However, the mutation of genes encoding H2A.Z and LHP1 attenuated and enhanced respectively the induction of target genes and altered H3K4me3. These results indicate that the induction of ethylene-responsive genes does not require immediate modulation of H3K4me3 and H3K27me3 and dissociation of LHP1 and H2A.Z from the targets, and suggest that the chromatin structure of the genes before induction is committed for transcriptional activation and that H3K4me3 is not required for ethylene-responsive gene activation, but may serve as a mark for gene activity.

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Section: Discussionmentioning

confidence: 74%

The Role of Histone Methylation and H2A.Z Occupancy during Rapid Activation of Ethylene Responsive Genes

Shen

Silva

et al. 2011

PLoS ONE

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“…Seedlings were transplanted to soil or harvested for experiments. Vernalization treatment was performed as previously described (Kim et al, 2010). Non-vernalized seedlings were grown for 11 days after germination under short day condition.…”

Section: Methodsmentioning

confidence: 99%

“…Cross-linked seedling plants were ground in liquid nitrogen and used for further ChIP experiments as previously described (Kim et al, 2010). To measure specificity of CLF antibody, we performed ChIP assay between WT and clf-29 mutants.…”

Section: Methodsmentioning

confidence: 99%

Vernalization-Triggered Intragenic Chromatin Loop Formation by Long Noncoding RNAs

2017

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SUMMARY Long noncoding RNAs (lncRNAs) affect gene regulation through structural and regulatory interactions with associated proteins. The Polycomb complex often binds to lncRNAs in eukaryotes and a lncRNA, COLDAIR, associates with Polycomb to mediate silencing of the floral repressor, FLOWERING LOCUS C (FLC) during the process of vernalization in Arabidopsis. Here we identified an additional Polycomb-binding lncRNA, COLDWRAP. COLDWRAP is derived from the repressed promoter of FLC and is necessary for the establishment of stable repressed state of FLC by vernalization. Both COLDAIR and COLDWRAP are required to form a repressive intragenic chromatin loop at the FLC locus by vernalization. Our results indicate that vernalization–mediated Polycomb silencing is coordinated by lncRNAs in a cooperative manner to form a stable repressive chromatin structure.

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“…Accordingly, Arabidopsis Trithorax1, which is involved in H3K4 trimethylation, was found to be necessary for gene induction by stress signals (Alvarez-Venegas and Avramova, 2005;Alvarez-Venegas et al, 2007;Ding et al, 2011). Conversely, H3K27me3 was removed from several inducible genes upon application of the inductive signals (Kwon et al, 2009;Kim et al, 2010), implying that active removal of this histone methylation mark may be associated with gene induction. However, the role of H3K27me3 demethylation in stress-responsive gene activation and stress tolerance has not been clarified.…”

Section: Introductionmentioning

confidence: 98%

Jumonji C Domain Protein JMJ705-Mediated Removal of Histone H3 Lysine 27 Trimethylation Is Involved in Defense-Related Gene Activation in Rice

et al. 2013

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Vernalization-Mediated VIN3 Induction Overcomes the LIKE-HETEROCHROMATIN PROTEIN1/POLYCOMB REPRESSION COMPLEX2-Mediated Epigenetic Repression

Cited by 42 publications

References 51 publications

The Role of Histone Methylation and H2A.Z Occupancy during Rapid Activation of Ethylene Responsive Genes

The Role of Histone Methylation and H2A.Z Occupancy during Rapid Activation of Ethylene Responsive Genes

Vernalization-Triggered Intragenic Chromatin Loop Formation by Long Noncoding RNAs

Jumonji C Domain Protein JMJ705-Mediated Removal of Histone H3 Lysine 27 Trimethylation Is Involved in Defense-Related Gene Activation in Rice

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