Vermiculite Influence on Growth and Storage of Mesorhizobium Ciceri H-12 in Heterophase Preparation

Abstract

It was established that vermiculite with particle size up to 1 mm (2% of the volume of the medium) is an important technology component for the production of heterophase preparation of M. ciceri H-12 with bacterial titer of 6,9 – 8,0 billion/ml, retention of cell viability duration and nodular activity to up to three months.

Microbial Interaction with Clay Minerals and Its Environmental and Biotechnological Implications

“…The addition of vermiculite (5 g/L) also stimulated B. subtilis IMV B-7023 growth, increasing cell numbers by almost 50%. Kameneva (2009) showed that vermiculite with a particle size up to 1 mm in diameter and concentration of 2% (w/v) is an important component for the biotechnological production of heterophase microbial preparation of nitrogen-fixing nodular bacteria Mesorhizobium ciceri H-12. Bacterial titer was 6.9-8.0 billion/mL, and cell viability and nodular activity were maintained for three months.…”

“…Bacterial titer was 6.9-8.0 billion/mL, and cell viability and nodular activity were maintained for three months. The microbial interactions with clay minerals with beneficial biological effects can successfully help the paramount task of improving the quality of microbial preparations of agriculturally valuable bacteria in order to ensure a high number of viable microbial cells in the preparation and the long-term preservation of their viability [246].…”