Verification of the Functional Antioxidant Activity and Antimelanogenic Properties of Extracts of Poria cocos Mycelium Fermented with Freeze-Dried Plum Powder

Abstract

Here we examine the effects of extracts of Poria cocos mycelium fermented with freeze-dried plum powder (PPE) on the α-melanocyte stimulating hormone (α-MSH)-stimulated melanogenesis in cultured murine B16 melanoma cells (B16 cells), relative to the effects of Prunus extract. We found that an extract of Prunus fermentation showed significant inhibition of melanogenesis and tyrosinase activity with no effect on cell proliferation and was more active compared to Prunus extract alone. Furthermore, we confirmed that medium containing 3% Prunus was the optimal culture substrate for fermentation with Poria cocos. These results provide evidence that Prunus fermentation extract affects skin whiting in murine B16 melanoma cells (B16 cells). Prunus contains rutin, oxalic acid, succinic acid, and fumaric acid, which help in digestion and fatigue recovery. The rutin of Prunus mume is reported to have antioxidant and anti-inflammatory effects. Also, Prunus extract has a tyrosinase inhibitory activity for skin whiting through its antioxidant activity. Therefore, we believe the Prunus extract for Poria cocos fermentation can be provided as a potential mediator to induce skin whiting.

Comprehensive Review of Phytochemical Constituents, Pharmacological Properties, and Clinical Applications of Prunus mume

“…Compounds in the P. mume extract, especially acylated quinic acid, inhibited melanogenesis and showed no cytotoxicity in theophylline-stimulated B16 melanoma cells (Nakamura et al, 2013a;Pi and Lee, 2017), indicating that P. mume might possess a skin-whitening effect. Interestingly, the effect can be strengthened by fermentation with Poria cocos mycelium (Kang et al, 2019). In folk remedies, P. mume has been reported to promote salivation.…”

Integrated Chinese and western medicine for acute guillain-barré syndrome treatment

“…applied two strategies: tonifying-Qi and removing-dampheat. Astragalus and Poria cocos are widely used and low-cost tonifying-Qi herbs in China [39,43], and licorice is a tonifying-Qi drug with a detoxifcation capability (towards the damp-heat evil) [44,45]; besides, Poria cocos can also fight the damp evil and accelerate the recovery of limb function [46,47]; atractylodes and semen coicis are frequently symptoms including extensive peripheral nerve demyelination and inflammatory response, motor nerve damage, and even vegetative nerve damage. The performance of these dysfunctions is in consistency with damp-heat syndromes, and theoretically (in TCM), the patients are under the condition of Qi lacking.…”

Penggunaan Ester Asam Fumarat Oral Dalam Pengobatan Kasus Dermatologi

“…Studi ini menyimpulkan bahwa DMF bekerja dengan menghambat masuknya NFkB ke dalam sel dan invasi berikutnya dan metastasis sel melanoma. [32] Dalam penelitian berikutnya, sel melanoma yang disuntikkan secara intradermal menunjukkan metastasis tertunda ke nodus sentinel ketika diobati dengan dacarbazine (DTIC) dan DMF. Kombinasi terapi ini juga mengurangi kepadatan pembuluh getah bening pada tumor primer ketika dievaluasi dengan PCR real-time dan imunohistokimia.…”