Verification of a New Pathogen Detection Method by PostSonication Next-Generation Sequencing with Quantitative Real-Time PCR for the Diagnosis of Orthopedic Biofilm Infection
Narumi Ueda,
Jun Inoue,
Kazuyuki Okuda
et al.
Abstract:Next-generation sequencing (NGS), are recommended for the unconfirmed diagnosis of periprosthetic joint infection (PJI), but initial assessment using quantitative methods is required to determine whether microorganisms are present, before embarking on sequencing approaches. This study aimed to validate a new diagnostic approach for orthopedic biofilm infections that combines quantitative methods as quantitative real-time polymerase chain reaction (qPCR) with NGS. This study sequenced 16 clinical samples from o… Show more
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