Verhandlungen Ärztlicher Gesellschaften

Lipid Mobilization from Human Subcutaneous Adipose Tissue in Vitro

“…glyccridc-glyccrol radioactivity was determined, 1 nil of the medium added to 1 ml acetone and the protein precipitate ccntrifuged. Aliquots of the supernatant were applied on silicic acid thin-layer chroniatoplates (Kicselgel nach Stahl, Merck, Darmstadt, Grrniany) and run in chloroform: acetone: 5N ammonia (10 : 80 : 10) (20) until a suitablc running distance was obtaincd. Glycerol and glucose were run separately as standards.…”

“…Aftcr running for a suitable distance the plates were dricd a t about 100" C until all smell of ammonia ha'd disappeared. The area of the plate on which standapds had been applied was sprayed with developing agents (20) with the other part of the plate covered. Areas corrcsponding to glycerol (R, = 0.3-0.4) and g l i i c o .~ iR, = 0.1) were then scraped into countiy vials and countcd as described by Snyder and Stcphcns (19).…”