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Böhm,; Schultz-Brauns,; Bíró, Oszkár; Kubig,; Lampe,; Wiele,; Dennig,; Natorp,; Fröhlich,; Helmreich,

doi:10.1007/bf01752052

Cited by 1 publication

(5 citation statements)

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“…NeuAc and Me4NeuAc were determined with the orcinol/Fe3+/HC1 reagent [14] and the periodic acid/ thiobarbiturate acid method [15] using microadaptations of the described procedures.…”

Section: Co Lo R Imr T R Ic a Nulysi~ Of Siulic A C Idsmentioning

confidence: 99%

“…sulfuric acidlmethanol (1 : 1, vlv), followed by heating (120 'C) for a few minutes. For cellulose, propan-1 -ol/butan-l-ol/O.l M HCI (2 : 1 : 1, v/vjv; solvent C) was used, and the sialic acid spots were stained with the orcinol/Fe"/HCl spray reagent [14].…”

Section: Thin-luyer Cliromutogsupliy O F Siulic Acidsmentioning

confidence: 99%

“…The residue was dissolved in tetrahydrofuran/water (3 : 2, v/v, 10 ml) and desalted at aminic acid (compound 111) eluted with a gradient (0 to 0.1 M) of pyridinium acetate, pH 6. Fractions were monitored for sialic acid [14] and tritium. Lyophilization of the fractions containing compound 111 gave a residue of 160 mg (88 %).…”

Section: Syn Tliesismentioning

confidence: 99%

“…Desialylation of fetuin was carried out essentially as described by Bernacki and Bosmann [16]. Fetuin (20 mg, containing 7.5 bound NeuAc) was treated with 0.025 sulfuric acid (2 ml) at 80 "C for 1 h. Desialylation was followed by a dual analysis of free sialic acid [15] and total sialic acid [14]. The solution was diluted with cold water (1 5 ml) and passed through a column of Dowex 1 x 4 (20 -50 mesh, formate form, 7 ml) at 4°C.…”

Section: Preparation Of' Asialojetuinmentioning

confidence: 99%

“…The mixture was analyzed by thin-layer chromatography on cellulose (solvent C) using reference sialic acids as tracers. Tracer spots were detected with the orcinoli Fe3 +/HCI spray reagent [14]. Chromatographic bands of the reaction products were cut laterally into 0.5-cm sections, the cellulose was scraped offer, transferred to scintillation vials and suspended in water (200 pl).…”

Section: Identification Of Sialic Acids Liberated By Virus Neuraminidusementioning

confidence: 99%

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Metabolism of 4‐O‐Methyl‐N‐acetylneuraminic Acid a Synthetic Sialic Acid

Beau

Schauer

1980

European Journal of Biochemistry

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1. 4-0-Methyl-N-acetylneuraminic acid shows a strong positive periodate-thiobarbiturate reaction. The mechanism of dye formation in this test for sialic acids is discussed in view of the studies already published.2. An efficient preparation of a tritium-labelled 4-0-methyl-N-acetylneuraminic acid, with high specific radioactivity, by an oxymercuration-demercuration procedure is presented.3. Sialyltransferase activities in microsomal fractions of equine liver using desialylated fetuin are studied. The enzyme activity, assayed in a radioactive procedure, shows an apparent K , value for CMP-N-acetylneuraminic acid of 0.7 mM, whereas this value is 3.4 mM for CMP-4-0-methyl-N-acetylneuraminic acid. Differences are also observed in the maximal velocity for the two substrates.4. The equine liver system can be used to prepare substantial amounts of fetuin containing radioactive N-acetylneuraminic acid or 4-0-methyl-N-acetylneuraminic acid. The isolated reaction products show similar sialic acid release by treatments with acid or fowl-plague virus neuraminidase. In contrast, 4-0-methyl-N-acetylneuraminic acid-fetuin displays a marked resistance to desialylation by Vibrio cholerae neuraminidase.5. Free 4-0-methyl-N-acetylneuraminic acid is completely resistant to the action of acylneuraminate pyruvate-lyase. It does not inhibit the enzymic cleavage reaction of N-acetylneuraminic acid.6. The influence of a substitution at C-4 of neuraminic acid on the enzymatic reaction mechanisms is discussed.The acylneuraminic acids (sialic acids), widely distributed in animal tissues, are important components of oligosaccharides, glycolipids and glycoproteins. The occurrence and nature of various Nacylated and O-acylated neuraminic acids is now well documented [l -31, and the pattern distribution is characteristic for the animal species. Sialic acids are implicated in many cellular processes [4], and the high number of the 0-acylated derivatives points to the importance of understanding the biological role of these substances. In a preliminary approach, a precise evaluation of the behaviour of the main enzymes involved in the metabolism of sialic acids is

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“…NeuAc and Me4NeuAc were determined with the orcinol/Fe3+/HC1 reagent [14] and the periodic acid/ thiobarbiturate acid method [15] using microadaptations of the described procedures.…”

Section: Co Lo R Imr T R Ic a Nulysi~ Of Siulic A C Idsmentioning

confidence: 99%

Section: Thin-luyer Cliromutogsupliy O F Siulic Acidsmentioning

confidence: 99%

Section: Syn Tliesismentioning

confidence: 99%

Section: Preparation Of' Asialojetuinmentioning

confidence: 99%

Section: Identification Of Sialic Acids Liberated By Virus Neuraminidusementioning

confidence: 99%

See 3 more Smart Citations