Vergleichende In-vitro-Studie zur Zytotoxizität klinisch eingesetzter Antiseptika

Hirsch, Tobias; Jacobsen, Frank; Rittig, A.; Goertz, Ole; Niederbichler, Andreas D.; Steinau, Hans-Ulrich; Seipp, Hans-Martin; Steinstraesser, Lars

doi:10.1007/s00105-009-1842-x

Cited by 41 publications

(24 citation statements)

References 15 publications

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“…MRSA or Enterococcus faecium [33] and it was shown that it does not induce adaptation in bacteria [34,35]. Yet, its application in wound treatment remains controversial as it may have potential systemic side effects and exert possible cytotoxic effects leading to impaired wound healing [36,37]. Here, loading of BNC with PI did not lead to adverse effects on the cells in vitro and contact tests revealed a strong antibacterial effect on S. aureus.…”

Section: Discussionmentioning

confidence: 92%

Antimicrobial functionalization of bacterial nanocellulose by loading with polihexanide and povidone-iodine

Wiegand

Moritz

Heßler

et al. 2015

J Mater Sci: Mater Med

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Bacterial nanocellulose (BNC) is chemically identical with plant cellulose but free of byproducts like lignin, pectin, and hemicelluloses, featuring a unique reticulate network of fine fibers. BNC sheets are mostly obtained by static cultivation. Now, a Horizontal Lift Reactor may provide a cost efficient method for mass production. This is of particular interest as BNC features several properties of an ideal wound dressing although it exhibits no bactericidal activity. Therefore, BNC was functionalized with the antiseptics povidone-iodine (PI) and polihexanide (PHMB). Drug loading and release, mechanical characteristics, biocompatibility, and antimicrobial efficacy were investigated. Antiseptics release was based on diffusion and swelling according to Ritger-Peppas equation. PI-loaded BNC demonstrated a delayed release compared to PHMB due to a high molar drug mass and structural changes induced by PI insertion into BNC that also increased the compressive strength of BNC samples. Biological assays demonstrated high biocompatibility of PI-loaded BNC in human keratinocytes but a distinctly lower antimicrobial activity against Staphylococcus aureus compared to PHMB-loaded BNC. Overall, BNC loaded with PHMB demonstrated a better therapeutic window. Moreover, compressive and tensile strength were not changed by incorporation of PHMB into BNC, and solidity during loading and release could be confirmed.

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Section: Discussionmentioning

confidence: 92%

Antimicrobial functionalization of bacterial nanocellulose by loading with polihexanide and povidone-iodine

Wiegand

Moritz

Heßler

et al. 2015

J Mater Sci: Mater Med

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“…Neurotoxicity has been reported for CHX [6][7][8][9]. Also PVP-I solution cannot be recommended for wound dressing of neural structures [10] and show reduced cell viability of fibroblasts in vitro [11] with probable delay in wound healing in the postoperative setting.…”

Section: Discussionmentioning

confidence: 95%

“…As a consequence, irrigation of penetrating wounds using Octenisept Ò resulted in aseptic tissue necrosis [19][20][21]. Besides, in vitro results indicate significantly reduced cell viability after octenidine treatment [11].…”

Section: Discussionmentioning

confidence: 97%

Eradication of MRSA skull base osteitis by combined treatment with antibiotics and sinonasal irrigation with sodium hypochlorite

Kuster

Krämer

Bremert

et al. 2015

Eur Arch Otorhinolaryngol

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“…Hence, PVP-iodine could be of interest for new applications in decontamination regimens or in the treatment of infected body cavities and joints [37]. However, its application in wound treatment remains controversial, as it may have potential systemic side effects and exert possible cytotoxic effects leading to impaired wound healing [39,40]. …”

Section: Discussionmentioning

confidence: 99%

Analysis of the Adaptation Capacity of <b><i>Staphylococcus aureus</i></b> to Commonly Used Antiseptics by Microplate Laser Nephelometry

Wiegand

Abel

Ruth

et al. 2012

Skin Pharmacol Physiol

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Background: Bacterial colonization and infection are important factors in compromised wound healing. Antiseptics have become an alternative for antimicrobial applications as antibiotic resistance is increasing; they have multiple targets with a broad spectrum of activity. Hence, the risk for developing resistance should be low. However, concerns have been raised that their growing use may result in bacteria that are less susceptible. Methods: The capacity of common antiseptics such as silver nitrate, polihexanide, octenidine, chlorhexidine and polyvinylpyrrolidone (PVP)-iodine to induce adaptation in a Staphylococcus aureus strain was analyzed in vitro using microplate laser nephelometry. S. aureus was repeatedly incubated with the respective half maximal inhibitory concentration (IC₅₀) over a time period of 100 days. The influence of the continued treatment was determined by in situ monitoring of changes in the dose-response curves and calculation of the current IC₅₀ values for the substances tested. Results: During the experiment, S. aureus quickly adapted to high concentrations of the antibiotic mupirocin during repeated treatment. Moreover, a significant increase of the IC₅₀ for silver nitrate was observed over time. On the other hand, no significant difference was observed for polihexanide or chlorhexidine. While the IC₅₀ for octenidine was also found to increase significantly, although the change was only marginal, reiterated incubation with PVP-iodine led to a decrease in the IC₅₀. Conclusion: Repeated treatment of S. aureus with polihexanide, chlorhexidine, octenidine and PVP-iodine did not trigger bacterial adaptation to these substances.

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Vergleichende In-vitro-Studie zur Zytotoxizität klinisch eingesetzter Antiseptika

Cited by 41 publications

References 15 publications

Antimicrobial functionalization of bacterial nanocellulose by loading with polihexanide and povidone-iodine

Antimicrobial functionalization of bacterial nanocellulose by loading with polihexanide and povidone-iodine

Eradication of MRSA skull base osteitis by combined treatment with antibiotics and sinonasal irrigation with sodium hypochlorite

Analysis of the Adaptation Capacity of <b><i>Staphylococcus aureus</i></b> to Commonly Used Antiseptics by Microplate Laser Nephelometry

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