Vergleichende histochemische, fluorescenzmikroskopische und elektronenoptische Untersuchungen zur Fr�hdiagnose des Herzinfarktes der Ratte

Hecht, A; Korb, G; David, Heinz

doi:10.1007/bf00957745

Cited by 52 publications

(4 citation statements)

References 28 publications

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“…Reeves The most striking ultrastructural changes on exposure to anoxia in the absence of glucose were the marked morphologic changes in the mitochondria and the dilatation of the longitudinal tubules. It is notable that similar changes in mitochondria and longitudinal tubules have been observed in experimental myocardial ischemia (19)(20)(21)(22)(23). The mitochondria are the cellular sites of aerobic energy generation l)v oxidatixve phosphorylation.…”

Section: Discussionmentioning

confidence: 65%

Role of anaerobic metabolism in the preservation of functional capacity and structure of anoxic myocardium

Weissler¹,

Kruger²,

Baba³

et al. 1968

J. Clin. Invest.

155

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A B ST R A CT Employing an isolated perfused rat heart preparation, we investigated the contriblution of anaerobic metabolic energy to the lerformance, recoverability, and ultrastructure of the heart perfused at 320C in 5% albumin in Krebs-Ringer Bicarbonate solution. During exposure to anoxia for 30 min, inclusion in the perfusate of the anaerobic substrate, glucose, resulted in marked improvement in electrical and mechanical performance of the heart and in enhanced recovery during the subsequent period of reoxygeiiation. Lactate production was fivefold greater in the glucose-supported anoxic heart than in the anoxic heart without glucose. Electron microscope sections of the hearts exposed to anoxia in the absence of glucose anaerobically derived metabolic energy to the electrical and mechanical performance of the heart, to functional recoverability after exposure to anoxia, or to the prevention of intracellular structural alterations accompanying anoxic exposure.Induction of myocardial hypoxia by simple cessation of coronary perfusion, in vivo or in vitro, does not permit a steady supply of anaerobic substrate to the myocardium. Furthermore, the effects of accumulated end products of anaerobic metabolism are not controlled under these circumstances. Studies with perfused myocardial strips, which avoid these difficulties, do not yield information on spontaneous electrical activity or on electromechanical integration of cardiac function. With these considerations in mind the present studies on the effects of anoxia on the isolated perfused rat heart were initiated. This preparation permits study of electrical and mechanical performance of the isolated heart during relatively prolonged experiments and instantaneous addition or deletion of anaerobic substrate while perfusion is maintained at a constant rate. The studies were designed to determine the degree to which the presence of the anaerobic substrate, glucose, affects the performance, recoverability, and ultrastructure of the perfused anoxic heart. METHODSThe hearts used in these studies were removed from male albino (Wistar) rats, weighing from 180 to 235 g. The rats were fed ad lib. with standard Purina laboratory chow until they were sacrificed. The animals were decapitated, the chest opened within 30 sec with a subxiphoid incision, and the heart flooded immediately with ice-cold Ringer's solution. This maneuver produced rapidThe Journal of Clinical Investigation Volume 47 1968 403 cessation of the heart beat, after which the heart was dissected from the mediastinum with an attached aortic stump 5 mm long. The heart was then mounted on the perfusion cannula which consisted of a No. 17 TW needle with a rounded and smoothed end and notched 3 mm above the tip. The tip of the cannula was placed in the aortic root which was attached firmly via a silk 3-0 ligature applied to the notch in the cannula. The time from extirpation of the heart to in vitro perfusion was 3-5 min.The perfusion system, as illustrated in Fig. 1, was recirculating in type and consisted o...

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Section: Discussionmentioning

confidence: 65%

Role of anaerobic metabolism in the preservation of functional capacity and structure of anoxic myocardium

Weissler¹,

Kruger²,

Baba³

et al. 1968

J. Clin. Invest.

155

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“…Changes in myocardial ul trastructure after permanent occlusion of a coronary artery have been demonstrated in the rat [9,16,30], rabbit [14], dog [18,27,40,80], and cat [44,45]. These changes occur at similar times in all 4 species.…”

mentioning

confidence: 69%

Myocardial Ultrastructure in Acute and Chronic Hypoxia

Ferrans¹,

Roberts²

1971

Cardiology

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“…The decrease in affinity of A bands forLevanol fast cyanine 5RN in infarcts within 1-3 h after ligation of a coronary artery apparently parallels structural changes reported in electron microscopic studies. Within 30 min after ligation of a coronary artery myofilaments showed longitudinal cracking and separation (Caulfield & Klionsky, 1959) and granular disintegration (Hecht et al, 1961). Within 2-4 h after ligation myofibrils were no longer sharply outlined or individually distinct (Bryant et al, 1958).…”

Section: Cowelation Of Staining Patterns With Chemical and Electron-mmentioning

confidence: 99%

“…During the last decade electron microscopists have described alterations in cardiac muscle fibres within 1 h after onset of ischemia (Caulfield & Klionsky, 1959;Hecht et al, 1961;Bryant et al, 1958;Hoelscher et al, 1961). Yet conventional staining methods do not show changes in infarcts within 6 h after occlusion of a coronary artery (Kent & Diseker, 1955;Robbins, 1962;Morehead, 1965).…”

Section: Introductionmentioning

confidence: 99%

A combined PAS‐myofibril stain for demonstration of early lesions of striated muscle

Puchtler

Waldrop

Terry

et al. 1969

Journal of Microscopy

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To facilitate studies of early myocardial lesions a method was developed for simultaneous demonstration of saccharides and muscle proteins. Deparaffinized sections were treated consecutively with the PAS reaction, tannic acid, phosphomolybdic acid and Levanol fast cyanine 5RN. A and Z bands in normal muscle were stained blue. I bands were coloured yellow. Under various pathological conditions A and Z bands lost their affinity for Levanol fast cyanine 5RN, with and without appearance of PAS-positive material in the Z bands.Comparison of staining patterns with chemical, electron microscopic and X-ray diffraction data indicated that Levanol fast cyanine 5RN is bound by myosin and tropomyosin B. This affinity is lost when the protein structure is altered. The origin of the PAS-positive material appearing in the Z bands could not yet be determined.

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Vergleichende histochemische, fluorescenzmikroskopische und elektronenoptische Untersuchungen zur Fr�hdiagnose des Herzinfarktes der Ratte

Cited by 52 publications

References 28 publications

Role of anaerobic metabolism in the preservation of functional capacity and structure of anoxic myocardium

Role of anaerobic metabolism in the preservation of functional capacity and structure of anoxic myocardium

Myocardial Ultrastructure in Acute and Chronic Hypoxia

A combined PAS‐myofibril stain for demonstration of early lesions of striated muscle

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